国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2012年
7期
513-516
,共4页
秦艳艳%杜永成%胡晓芸%邢爱萍%赵瑛娟
秦豔豔%杜永成%鬍曉蕓%邢愛萍%趙瑛娟
진염염%두영성%호효예%형애평%조영연
烟雾暴露%重塑%过氧化物酶体增殖物激活受体γ
煙霧暴露%重塑%過氧化物酶體增殖物激活受體γ
연무폭로%중소%과양화물매체증식물격활수체γ
Cigarette smoke exposure%Remodeling%Peroxisome proliferator-activated receptor γ
目的 探讨烟雾暴露对大鼠肺血管过氧化物酶体增殖物激活受体γ(PPAR-γ)表达的影响及其在肺血管重塑中的作用.方法 雄性SD大鼠30只,随机分为对照组、烟雾暴露6周组、烟雾暴露12周组,建立大鼠烟雾暴露模型.HE染色观察各组大鼠肺组织形态学改变,测量肺血管管壁厚度与血管外径的比值(WT％)及管壁面积与血管总面积的比值(WA％);免疫组织化学染色检测肺血管PPAR-γ的表达量,并分析各指标的相关性.结果 ①烟雾暴露6周组WT％及WA％[(45.61±2.43)％及(59.69±12.59)％]与烟雾暴露12周组[(54.23±6.22)％及(76.26±8.36)％]均高于对照组[(32.89±7.65)％及(33.05±14.84)％],差异均有统计学意义(P值均＜0.05);烟雾暴露12周组WT％及WA％高于烟雾暴露6周组,差异均有统计学意义(P值均＜0.05);②烟雾暴露6周组与烟雾暴露12周组PPARγ蛋白表达[(0.48±0.08),(0.37±0.07)]均低于对照组(0.51±0.07),差异均有统计学意义(P值均＜0.05);烟雾暴露12周组PPAR-γ蛋白表达低于烟雾暴露6周组,差异有统计学意义(P＜0.05);③肺血管PPAR-γ与WT％及WA％呈负相关(r值分别为-0.715和-0.683,P值均＜0.05).结论 烟雾暴露可下调大鼠肺血管PPAR-γ的表达,参与肺血管重塑.
目的 探討煙霧暴露對大鼠肺血管過氧化物酶體增殖物激活受體γ(PPAR-γ)錶達的影響及其在肺血管重塑中的作用.方法 雄性SD大鼠30隻,隨機分為對照組、煙霧暴露6週組、煙霧暴露12週組,建立大鼠煙霧暴露模型.HE染色觀察各組大鼠肺組織形態學改變,測量肺血管管壁厚度與血管外徑的比值(WT％)及管壁麵積與血管總麵積的比值(WA％);免疫組織化學染色檢測肺血管PPAR-γ的錶達量,併分析各指標的相關性.結果 ①煙霧暴露6週組WT％及WA％[(45.61±2.43)％及(59.69±12.59)％]與煙霧暴露12週組[(54.23±6.22)％及(76.26±8.36)％]均高于對照組[(32.89±7.65)％及(33.05±14.84)％],差異均有統計學意義(P值均＜0.05);煙霧暴露12週組WT％及WA％高于煙霧暴露6週組,差異均有統計學意義(P值均＜0.05);②煙霧暴露6週組與煙霧暴露12週組PPARγ蛋白錶達[(0.48±0.08),(0.37±0.07)]均低于對照組(0.51±0.07),差異均有統計學意義(P值均＜0.05);煙霧暴露12週組PPAR-γ蛋白錶達低于煙霧暴露6週組,差異有統計學意義(P＜0.05);③肺血管PPAR-γ與WT％及WA％呈負相關(r值分彆為-0.715和-0.683,P值均＜0.05).結論 煙霧暴露可下調大鼠肺血管PPAR-γ的錶達,參與肺血管重塑.
목적 탐토연무폭로대대서폐혈관과양화물매체증식물격활수체γ(PPAR-γ)표체적영향급기재폐혈관중소중적작용.방법 웅성SD대서30지,수궤분위대조조、연무폭로6주조、연무폭로12주조,건립대서연무폭로모형.HE염색관찰각조대서폐조직형태학개변,측량폐혈관관벽후도여혈관외경적비치(WT％)급관벽면적여혈관총면적적비치(WA％);면역조직화학염색검측폐혈관PPAR-γ적표체량,병분석각지표적상관성.결과 ①연무폭로6주조WT％급WA％[(45.61±2.43)％급(59.69±12.59)％]여연무폭로12주조[(54.23±6.22)％급(76.26±8.36)％]균고우대조조[(32.89±7.65)％급(33.05±14.84)％],차이균유통계학의의(P치균＜0.05);연무폭로12주조WT％급WA％고우연무폭로6주조,차이균유통계학의의(P치균＜0.05);②연무폭로6주조여연무폭로12주조PPARγ단백표체[(0.48±0.08),(0.37±0.07)]균저우대조조(0.51±0.07),차이균유통계학의의(P치균＜0.05);연무폭로12주조PPAR-γ단백표체저우연무폭로6주조,차이유통계학의의(P＜0.05);③폐혈관PPAR-γ여WT％급WA％정부상관(r치분별위-0.715화-0.683,P치균＜0.05).결론 연무폭로가하조대서폐혈관PPAR-γ적표체,삼여폐혈관중소.
Objective To investigate the expression variation of peroxisome proliferator-activated receptor γ (PPAR-γ) in pulmonary vasculature in rats exposed to cigarette smoke and its role in pulmonary vascular remodeling.Methods 30 male SD rats were randomly divided into a control group,a smoke exposure for 6 weeks group,a smoke exposure for 12 weeks group,with 10 rats in each group.The ratio of pulmonary vascular wall thickness/vascular external diameter (WT％)and the ratio of pulmonary vascular wall area/total pulmonary vascular area (WA％)were measured by the image analysis system.The expression of PPAR-γ protein in pulmonary vascular of each group was detected by Immunohistochemistry.Results ①WT％ and WA％ increased significantly in the smoke exposure for 6 weeks group [(45.61 ± 2.43)％,(59.69±12.59)％] and smoke exposure for 12 weeks group [(54.23 ±6.22) ％,(76.26±8.36)％] compared to those of control group [(32.89±7.65)％,(33.05±14.84)％] (all P＜0.05). The WT％ and WA％ in the smoke exposure for 12 weeks group increased significantly compared to those of smoke exposure for 6 weeks group (all P ＜0.05).②The expression of PPAR-γ protein in pulmonary vascular decreased significantly in the smoke exposure for 6 weeks group (0.48±0.08)and the smoke exposure for 12 weeks group (0.37±0.07) compared to control group (0.51±0.07)(all P＜0.05).The expression of PPAR-γ protein decreased in the smoke exposure for 12 weeks group compared to the smoke exposure for 6 weeks group (P＜0.05).③The expression of PPAR-γ protein in pulmonary vascular was respectively negatively correlated with WT％ and WA％ ( r =-0.715 and -0.683)(all P＜ 0.05).Conclusions By down-regulating the expression of PPAR-γ protein in pulmonary vascular,cigarette smoke exposure induces the remodeling of pulmonary vascular.
