CAJ | 학술논문

目的:从神经功能恢复和髓鞘形态学变化的角度探讨针刺对受损脑髓鞘的保护作用.方法:89只成年雄性SD大鼠随机分为正常组(n=5)、假手术组(n=21)、模型组(n=21)、早期针刺组(n=21)、晚期针刺组(n=21).用线栓法制作大脑中动脉闭塞模型,分别于缺血后不同时间点进行针刺,应用神经功能缺损评分法、Pal-Weigert髓鞘染色法观察神经功能和缺血灶髓鞘形态学改变.结果:模型组在术后2.5 h神经功能缺损评分显著增高(P＜0.05),随着时间推移,神经功能缺损评分有所下降,内囊有明显的髓鞘脱失且恢复缓慢.与模型组相比,针刺组各个时间点大鼠神经功能缺损评分下降速度较快,3 d时下降显著(P＜0.05);髓鞘脱失程度显著减轻.与晚期针刺组比较,早期针刺组神经功能缺损评分下降速度较快(P＜0.05),且第1天时内囊髓鞘脱失似乎更轻.结论:早期针刺有利于脑缺血后髓鞘再生和神经功能恢复.
목적:종신경공능회복화수초형태학변화적각도탐토침자대수손뇌수초적보호작용.방법:89지성년웅성SD대서수궤분위정상조(n=5)、가수술조(n=21)、모형조(n=21)、조기침자조(n=21)、만기침자조(n=21).용선전법제작대뇌중동맥폐새모형,분별우결혈후불동시간점진행침자,응용신경공능결손평분법、Pal-Weigert수초염색법관찰신경공능화결혈조수초형태학개변.결과:모형조재술후2.5 h신경공능결손평분현저증고(P＜0.05),수착시간추이,신경공능결손평분유소하강,내낭유명현적수초탈실차회복완만.여모형조상비,침자조각개시간점대서신경공능결손평분하강속도교쾌,3 d시하강현저(P＜0.05);수초탈실정도현저감경.여만기침자조비교,조기침자조신경공능결손평분하강속도교쾌(P＜0.05),차제1천시내낭수초탈실사호경경.결론:조기침자유리우뇌결혈후수초재생화신경공능회복.
Objective:To investigate the protection of acupuncture on damaged myelin sheath from the poinr of view of neurological functional recovery and morphological changes of the myelin sheath.Methods:Eighty-nine adult male Sprague-Dawley rats were randomly allocated to normal (n=5),sham-operation (n=21),model (n=21),early acupuncture (n=21) and late acupancture (n=21) groups.A model of middle cerebral artery occlusion was induced by the intraluminal filament method.Acupuncture was performed at different time points after ischemia.Neurological function and morphological changes of the myelin sheath of ischemic focus were observed by using the methods of neurological deficit scores and Pal-Weigert's myelin staining.Results:Neurological deficit scores at 2.5 hours after the procedure in the model group increased significantly (P＜0.05).qhe scores decreased somewhat as the time elapsed.The internal capsule had obvious demyelination and recovered slowly.Neurological deficit scores in rats at each time point decreased faster in the acupuncture group compared with the model group,and they decreased significantly at day 3 (P＜0.05);the extent of demyelination was significantly alleviated.Neurological deficit scores decreased faster in the early acupuncture group compared with the late acupuncture group (P＜0.05);and demyelination in the early acupuncture group seemed milder at day one.Conclusions:Early acupuncture is beneficial to remyelination and neurological functional recovery after cerebral ischemia.