中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2008年
5期
294-297
,共4页
黎润光%邵景范%魏明发%洪振亚%谢柏臻%杨小进%张文%陈超%王子民%袁继炎
黎潤光%邵景範%魏明髮%洪振亞%謝柏臻%楊小進%張文%陳超%王子民%袁繼炎
려윤광%소경범%위명발%홍진아%사백진%양소진%장문%진초%왕자민%원계염
间质干细胞%牵张应力%钙通道
間質榦細胞%牽張應力%鈣通道
간질간세포%견장응력%개통도
Mesenchymal stem cells%Mechanical strain%Calcium channel
目的 观察体外小儿骨髓间充质干细胞(MSCs)对周期性牵张应力的早期应答反应.方法 分离并培养小儿MSCs,利用体外细胞牵张应力装置对第三～六代细胞加载短期力学信号,检测各组细胞内Oa2+以及早期反应基因蛋白(c-fos、c-jun和c-myc)的表达情况.结果 应力干预MSCs后,细胞内Ca3+浓度在受力1min后即增高,荧光强度是对照组的164.63%,3、5min升高更明显,干预10 min后Ca2+浓度升高的幅度有下降趋势;c-fos、c-jun和c-myc基因蛋白在未受力组细胞内不表达或微量表达,加载力学信号后,表达发生了明显的变化,随时间的延长,均表现为增强-持续-回复原来水平.结论 周期性牵张应力影响MSCs内Ca2+的浓度及早期反应基因蛋白(c-fos、c-jun和c-myc)的表达,推测c-fos、c-jun和c-myc参与了MSCs响应力学的信号传导途径,可能依赖Ca2+通道的活化来调控.
目的 觀察體外小兒骨髓間充質榦細胞(MSCs)對週期性牽張應力的早期應答反應.方法 分離併培養小兒MSCs,利用體外細胞牽張應力裝置對第三～六代細胞加載短期力學信號,檢測各組細胞內Oa2+以及早期反應基因蛋白(c-fos、c-jun和c-myc)的錶達情況.結果 應力榦預MSCs後,細胞內Ca3+濃度在受力1min後即增高,熒光彊度是對照組的164.63%,3、5min升高更明顯,榦預10 min後Ca2+濃度升高的幅度有下降趨勢;c-fos、c-jun和c-myc基因蛋白在未受力組細胞內不錶達或微量錶達,加載力學信號後,錶達髮生瞭明顯的變化,隨時間的延長,均錶現為增彊-持續-迴複原來水平.結論 週期性牽張應力影響MSCs內Ca2+的濃度及早期反應基因蛋白(c-fos、c-jun和c-myc)的錶達,推測c-fos、c-jun和c-myc參與瞭MSCs響應力學的信號傳導途徑,可能依賴Ca2+通道的活化來調控.
목적 관찰체외소인골수간충질간세포(MSCs)대주기성견장응력적조기응답반응.방법 분리병배양소인MSCs,이용체외세포견장응력장치대제삼～륙대세포가재단기역학신호,검측각조세포내Oa2+이급조기반응기인단백(c-fos、c-jun화c-myc)적표체정황.결과 응력간예MSCs후,세포내Ca3+농도재수력1min후즉증고,형광강도시대조조적164.63%,3、5min승고경명현,간예10 min후Ca2+농도승고적폭도유하강추세;c-fos、c-jun화c-myc기인단백재미수력조세포내불표체혹미량표체,가재역학신호후,표체발생료명현적변화,수시간적연장,균표현위증강-지속-회복원래수평.결론 주기성견장응력영향MSCs내Ca2+적농도급조기반응기인단백(c-fos、c-jun화c-myc)적표체,추측c-fos、c-jun화c-myc삼여료MSCs향응역학적신호전도도경,가능의뢰Ca2+통도적활화래조공.
Objective To study the early responses of bone marrow mesenehymal stem cells(MSCs)to mechanical strain in children.Methods The MSCs were isolated from children and cultured primarily.After 3 to 6 generations,the MSCs were stretched mechanically.Intracellular free [Ca2+]was measured with flow cytometry.The early-response genes/proteins(c-fos、c-jun、c-myc)were examined with RT-PCR、immunocytochemistry and Western Blot.Results Intracellular free[Ca2+]i was elevated after one minute of mechanical stretch. The fluorescence intensity was 164.63% that of the control group.The increase was more prominent at three and five minutes.It became less obvious at 10 minutes.The application of mechanical strain also increased intracellular c-fos、c-jun、c-myc mRNA/protein levels.Conclusions These data suggest mechanical strain could affect MSCs[Ca2+]i and earlyresponse genes/proteins levels of c-fos、c-jun、c-myc,implying that they are lnvolved in the early mechanical strain transduction pathways,possibly by activating calcium ion channel in MSCs.
