CAJ | 학술논문

目的探讨念珠菌黏附性与生物膜形成的关系。方法将8株念珠菌和1株酿酒酵母均分别在YPD液体培养基和琼脂培养基中培养，观察其絮凝沉淀和黏附现象，进一步在96孔微量培养皿中建立生物膜模型，测定生物膜的生长动力学变化。结果 9株菌株均具有絮凝沉淀的能力，经轻微振荡后白念珠菌、乳酒念珠菌、光滑念珠菌和热带念珠菌不易重新悬浮，而酿酒酵母容易重新悬浮。9株菌株均能黏附于YPD琼脂培养基的表面，白念珠菌、乳酒念珠菌、光滑念珠菌和热带念珠菌的黏附性强，而其他菌株的黏附性弱。常见的致病性念珠菌都能形成生物膜，且生物膜念珠菌活性随培养时间延长而增加。白念珠菌和乳酒念珠菌形成生物膜的能力较其他菌株强(P＜0.05)；热带念珠菌和光滑念珠菌形成生物膜的能力较克柔念珠菌、近平滑念珠菌和高里念珠菌强(P＜0.05)。不致病的酿酒酵母不能形成生物膜。结论念珠菌具有黏附的能力，且强弱不同。致病性念珠菌具有形成生物膜的能力，且强弱不同。念珠菌形成生物膜的能力与其黏附力呈正相关。
목적 탐토념주균점부성여생물막형성적관계。방법 장8주념주균화1주양주효모균분별재YPD액체배양기화경지배양기중배양，관찰기서응침정화점부현상，진일보재96공미량배양명중건립생물막모형，측정생물막적생장동역학변화。결과 9주균주균구유서응침정적능력，경경미진탕후백념주균、유주념주균、광활념주균화열대념주균불역중신현부，이양주효모용역중신현부。9주균주균능점부우YPD경지배양기적표면，백념주균、유주념주균、광활념주균화열대념주균적점부성강，이기타균주적점부성약。상견적치병성념주균도능형성생물막，차생물막념주균활성수배양시간연장이증가。백념주균화유주념주균형성생물막적능력교기타균주강(P＜0.05)；열대념주균화광활념주균형성생물막적능력교극유념주균、근평활념주균화고리념주균강(P＜0.05)。불치병적양주효모불능형성생물막。결론 념주균구유점부적능력，차강약불동。치병성념주균구유형성생물막적능력，차강약불동。념주균형성생물막적능력여기점부력정정상관。
Objective To assess the relationship between Candida adhesivity and biofilm formation. Methods Eight Candida strains belonging to 8 species and 1 Saccharomyces cerevisiae strain were cultured in yeast peptone dextrose (YPD) fluid and agar medium respectively. The flocculation and adhesion of Candida were observed. Candida biofilm models were developed in 96-well microculture plates. The kinetics of biofilm formation was measured. Results All the 9 fungal strains had flocculation capability and could adhere to the surface of the yeast peptone dextrose agar medium. After mild shaking of the fluid medium, it is difficult for C. albicans, C. kefyr, C. glabrata and C. tropicalis to resuspend, but easy for Saccharomyces cerevisiae. The adhesivity of C. albicans, C. kefyr, C. glabrata and C. tropicalis was stronger than that of the other Candida strains. Common pathogenic Candida strains could form biofilm, and the metabolic activity of Candida cells in the biofilm increased over time. A significant increment was observed in the ability of C. albicans and C. kefyr to form biofilm compared with the other species (all P ＜ 0.05), and in that of C. tropicalis and C. glabrata compared with C. krusei, C. parapsilosis and C. gulliermondii (all P ＜ 0.05). The nonpathogenic Saccharomyces cerevisiae could not form biofilm. Conclusions Candida has the ability to adhere and form biofilm,and the ability varies with Candida species. Moreover, the ability to form biofilm positively correlates with the adhesivity of Candida.