中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2009年
3期
239-242
,共4页
周济华%张海林%王静林%杨卫红%米竹青%张云智%章域震%宋先毅%胡秋凌%董应宽%普文华%胡海梅%高丽芬%袁庆虹%亚红祥%冯云
週濟華%張海林%王靜林%楊衛紅%米竹青%張雲智%章域震%宋先毅%鬍鞦凌%董應寬%普文華%鬍海梅%高麗芬%袁慶虹%亞紅祥%馮雲
주제화%장해림%왕정림%양위홍%미죽청%장운지%장역진%송선의%호추릉%동응관%보문화%호해매%고려분%원경홍%아홍상%풍운
汉坦病毒%汉城型%宿主动物%分子流行病学
漢坦病毒%漢城型%宿主動物%分子流行病學
한탄병독%한성형%숙주동물%분자류행병학
Hantavirus%Seoul type%Host animal%Molecular epidemiology
目的 掌握汉坦病毒(HV)在云南省啮齿动物中的流行情况及其遗传特征.方法 采用笼夜法捕鼠,鼠肺标本用直接免疫荧光法检测HV抗原,用RT-PCR法检测HV核酸及分型,对产物进行序列测定,用PHYLIP软件包进行系统发生分析.结果 2005-2006年在云南省楚雄州10个县(市)居民区共捕获鼠类4属8种1421只,其中褐家鼠1056只,构成比高达74.31%,其次为黄胸鼠(19.70%).检测鼠肺组织1029份,HV抗原阳性53份,总带病毒率为5.15%,带病毒鼠种为褐家鼠(5.08%,38/748))、黄胸鼠(5.97%,12/201)、大足鼠(7.69%,2/26)和斯氏家鼠(6.25%,1/16).HV汉城型(SEO)核酸检测阳性21份(褐家鼠15份,黄胸鼠4份,大足鼠2份).获得的12个病毒S片段核苷酸序列与GenBank中国SEO型病毒R22、L99和HLD65株同源性较高,为87.1%～99.7%;与汉滩型(HTN)76-118株同源性仅为64.4%～69.1%.系统进化分析显示,楚雄州12个病毒S片段核苷酸序列与SEO型亲缘关系较近,与HTN型及其他型HV相距较远,证实这12份病毒标本均为SEO型病毒,并可分S1和S3两个亚型.结论 首次证实楚雄州10个县(市)广泛存在以褐家鼠为主要传染源的HFRS家鼠型疫源地及SEO型病毒的流行.分析发现SEO型不同亚型病毒的分布有其地域特点和独立性.
目的 掌握漢坦病毒(HV)在雲南省齧齒動物中的流行情況及其遺傳特徵.方法 採用籠夜法捕鼠,鼠肺標本用直接免疫熒光法檢測HV抗原,用RT-PCR法檢測HV覈痠及分型,對產物進行序列測定,用PHYLIP軟件包進行繫統髮生分析.結果 2005-2006年在雲南省楚雄州10箇縣(市)居民區共捕穫鼠類4屬8種1421隻,其中褐傢鼠1056隻,構成比高達74.31%,其次為黃胸鼠(19.70%).檢測鼠肺組織1029份,HV抗原暘性53份,總帶病毒率為5.15%,帶病毒鼠種為褐傢鼠(5.08%,38/748))、黃胸鼠(5.97%,12/201)、大足鼠(7.69%,2/26)和斯氏傢鼠(6.25%,1/16).HV漢城型(SEO)覈痠檢測暘性21份(褐傢鼠15份,黃胸鼠4份,大足鼠2份).穫得的12箇病毒S片段覈苷痠序列與GenBank中國SEO型病毒R22、L99和HLD65株同源性較高,為87.1%～99.7%;與漢灘型(HTN)76-118株同源性僅為64.4%～69.1%.繫統進化分析顯示,楚雄州12箇病毒S片段覈苷痠序列與SEO型親緣關繫較近,與HTN型及其他型HV相距較遠,證實這12份病毒標本均為SEO型病毒,併可分S1和S3兩箇亞型.結論 首次證實楚雄州10箇縣(市)廣汎存在以褐傢鼠為主要傳染源的HFRS傢鼠型疫源地及SEO型病毒的流行.分析髮現SEO型不同亞型病毒的分佈有其地域特點和獨立性.
목적 장악한탄병독(HV)재운남성교치동물중적류행정황급기유전특정.방법 채용롱야법포서,서폐표본용직접면역형광법검측HV항원,용RT-PCR법검측HV핵산급분형,대산물진행서렬측정,용PHYLIP연건포진행계통발생분석.결과 2005-2006년재운남성초웅주10개현(시)거민구공포획서류4속8충1421지,기중갈가서1056지,구성비고체74.31%,기차위황흉서(19.70%).검측서폐조직1029빈,HV항원양성53빈,총대병독솔위5.15%,대병독서충위갈가서(5.08%,38/748))、황흉서(5.97%,12/201)、대족서(7.69%,2/26)화사씨가서(6.25%,1/16).HV한성형(SEO)핵산검측양성21빈(갈가서15빈,황흉서4빈,대족서2빈).획득적12개병독S편단핵감산서렬여GenBank중국SEO형병독R22、L99화HLD65주동원성교고,위87.1%～99.7%;여한탄형(HTN)76-118주동원성부위64.4%～69.1%.계통진화분석현시,초웅주12개병독S편단핵감산서렬여SEO형친연관계교근,여HTN형급기타형HV상거교원,증실저12빈병독표본균위SEO형병독,병가분S1화S3량개아형.결론 수차증실초웅주10개현(시)엄범존재이갈가서위주요전염원적HFRS가서형역원지급SEO형병독적류행.분석발현SEO형불동아형병독적분포유기지역특점화독립성.
Objective To determine the hosts of hantavirus (HV) and its molecular epidemiological characteristics, to provide evidence for prevention and control on hemorrhagic fever with renal syndrome (HFRS). Methods Rodents were captured by a special trap within the residential area. The antigens of HV in lung tissues were detected by direct immuno-fluorescence assay (DFA). Nucleotide sequences of HV were amplified by RT-PCR with HV genotype-specific primer. The amplified genes were then sequenced. Phylogenetic tree were built on nucleotide sequence with Clusta1X 1.83 software. Results 1421 rodents were captured and classified into 8 species of 4 Genera in the epidemic area within 10 counties of Chuxiong prefecture, Yunnan province, between 2005 and 2006. Out of the 1421 rodents, 1056 (74.31%) of them were Rattus norvegicas and 280 (19.70%) belonged to Rattus flavipectus. The antigens of HV were detected by DFA in lung tissues and the total positive rate of HV was 5.15% (53/ 1029). After applying the sequencing nucleotide method to the 53 positive specimens, data showed that 21 specimens were positive and all of them belonged to Seoul type ( 15 samples were from Rattus norvegicus, 4 samples Rattasflavipectas, 2 samples Rattus nitidas). The partial S segments from 12 specimens were sequenced which appeared homologic with R22, L99 and HLD65 from GenBank in relatively high level (87.1%-99.7%). When compared to 76-118 strain of Hantaan type, their homologic degree was only 64.4%-69.1%. Results from Phylogenetic analysis showed that 12 specimens belonged to Seoul type. As for their homology, they were significantly similar to Seoul type and could be tentatively divided into two subtypes S1 and S3. Conclusion It was confirmed that the Seoul type virus, as HFRS' s pathogenetic agent mainly carried by rats, prevailed widely in Chuxiong prefecture. Owing to the local ecological environment, we also noticed the characteristics of different HV subtypes among Seoul type.
