CAJ | 학술논문

目的探讨肿瘤坏死因子α(tumor necrosis factorα,TNF-α)基因启动子区-308G/A、-857C/T和-1031T/C位点,白细胞介素6(interleukin-6)基因启动子-174G/C和-572C/G多态性与精神分裂症发生的相关性.方法采用聚合酶链反应-限制性片段长度多态性分析方法检测346例精神分裂症患者、323名健康对照各个多态性位点的基因型,采用SPSS 13.0进行统计学分析.结果 TNF-α基因-857C/T位点的基因型及等位基因频率分布在精神分裂症组与正常对照组均存在统计学意义(P＜0.05),其中,-857T的等位基因的频率在精神分裂症组均显著高于对照组(x2=9.414,P=0.002,OR=1.511,95%CI:1.160～1.969).-857C/T位点不同基因型患者之间的阳性和阴性症状量表总分及阴性症状差异存在显著性,且TT基因型的分值显著高于CC基因型(P＜0.05).结论 TNF-α基因-857C/T位点可能与精神分裂症的发病存在关联,其中,-857T等位基因可能是易感基因,并且-857C/T位点与阳性和阴性症状量表评分中阴性症状存在关联.
목적 탐토종류배사인자α(tumor necrosis factorα,TNF-α)기인계동자구-308G/A、-857C/T화-1031T/C위점,백세포개소6(interleukin-6)기인계동자-174G/C화-572C/G다태성여정신분렬증발생적상관성.방법 채용취합매련반응-한제성편단장도다태성분석방법검측346례정신분렬증환자、323명건강대조각개다태성위점적기인형,채용SPSS 13.0진행통계학분석.결과 TNF-α기인-857C/T위점적기인형급등위기인빈솔분포재정신분렬증조여정상대조조균존재통계학의의(P＜0.05),기중,-857T적등위기인적빈솔재정신분렬증조균현저고우대조조(x2=9.414,P=0.002,OR=1.511,95%CI:1.160～1.969).-857C/T위점불동기인형환자지간적양성화음성증상량표총분급음성증상차이존재현저성,차TT기인형적분치현저고우CC기인형(P＜0.05).결론 TNF-α기인-857C/T위점가능여정신분렬증적발병존재관련,기중,-857T등위기인가능시역감기인,병차-857C/T위점여양성화음성증상량표평분중음성증상존재관련.
Objective To investigate the relationship between the -308G/A, -857C/T and -1031T/C of tumor necrosis factor α gene (TNF-α), -174G/C and -572C/G of interleukin-6 gene (IL-6)polymorphisms and schizophrenia. Methods Genomic DNA was isolated from the venous blood leukocytes of 346 unrelated patients with schizophrenia and 323 healthy unrelated individuals (control group). All of the polymorphisms were genotyped by PCR-restriction fragment length polymorphisms (PCR-RFLP).Genotype and allele frequencies were analyzed by SPSS13. 0 software. Results There were significant differences in both allele and genotype frequencies of -857C/T of TNF-α gene between the schizophrenia and control groups (P＜0. 05). The allele T of -857C/T in schizophrenia group was significantly higher than that in control group (x2 = 9.414, P= 0. 002, OR= 1.511, 95% CI: 1. 160-1. 969). In addition, there were significant differences in the positive and negative syndrome scale (PANSS) total score and negative symptoms between the patients with different -857C/T genotypes, and the negative symptom score of TT genotype was significantly higher than that of CC genotype (P＜0.05). Conclusion There is an association between -857C/T of TNF-α gene and schizophrenia, individuals with T allele of -857C/T are susceptible to schizophrenia, and there is an association between -857C/T and negative symptom score.