中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2009年
6期
533-536
,共4页
肌肽%大鼠%亚硒酸钠%白内障%免疫电泳,双向
肌肽%大鼠%亞硒痠鈉%白內障%免疫電泳,雙嚮
기태%대서%아서산납%백내장%면역전영,쌍향
Carnosine%Rats%Sodium selenite%Cataract%Immunoelectrophoresis,two-dimensional
目的 研究L-肌肽防治大鼠硒性白内障的作用.方法 实验研究.用亚硒酸钠制作大鼠白内障模型,分为3组,每组9只,分别用50 g/L、20 g/L的L-肌肽滴眼液及生理盐水滴眼3周,观察大鼠晶状体变化情况.将大鼠白内障晶状体体外培养,分别加入1.00、0.10及0.01 g/L的L-肌肽无血清营养液,培养1周,观察大鼠晶状体变化.再将培养的大鼠白内障晶状体进行双向电泳实验.采用方差分析对各组间的均数进行比较.结果 大鼠用药1周,50g/L、20g/L的L-肌肽组和对照组的晶状体病变度数分别为2.22±0.65、2.39±0.98及2.83±0.38;50 g/L的L-肌肽组病变轻于对照组,差异有统计学意义(P=0.013).用药2周和3周,3组间晶状体病变差异无统计学意义(P>0.05).大鼠自内障晶状体培养1周,1.00、0.10及0.01 g/L的L-肌肽组晶状体病变与对照组差异无统计学意义(P>0.05).双向电泳结果显示:药物组蛋白总数为182,对照组为161,药物组高相对分子质量蛋白数量减少,低相对分子质量蛋白数量增多.结论 50 g/L的L-肌肽可抑制大鼠白内障早期病变的发展;肌肽在体外可使大鼠白内障品状体蛋白发生变化,对白内障晶状体病变程度作用不明显.
目的 研究L-肌肽防治大鼠硒性白內障的作用.方法 實驗研究.用亞硒痠鈉製作大鼠白內障模型,分為3組,每組9隻,分彆用50 g/L、20 g/L的L-肌肽滴眼液及生理鹽水滴眼3週,觀察大鼠晶狀體變化情況.將大鼠白內障晶狀體體外培養,分彆加入1.00、0.10及0.01 g/L的L-肌肽無血清營養液,培養1週,觀察大鼠晶狀體變化.再將培養的大鼠白內障晶狀體進行雙嚮電泳實驗.採用方差分析對各組間的均數進行比較.結果 大鼠用藥1週,50g/L、20g/L的L-肌肽組和對照組的晶狀體病變度數分彆為2.22±0.65、2.39±0.98及2.83±0.38;50 g/L的L-肌肽組病變輕于對照組,差異有統計學意義(P=0.013).用藥2週和3週,3組間晶狀體病變差異無統計學意義(P>0.05).大鼠自內障晶狀體培養1週,1.00、0.10及0.01 g/L的L-肌肽組晶狀體病變與對照組差異無統計學意義(P>0.05).雙嚮電泳結果顯示:藥物組蛋白總數為182,對照組為161,藥物組高相對分子質量蛋白數量減少,低相對分子質量蛋白數量增多.結論 50 g/L的L-肌肽可抑製大鼠白內障早期病變的髮展;肌肽在體外可使大鼠白內障品狀體蛋白髮生變化,對白內障晶狀體病變程度作用不明顯.
목적 연구L-기태방치대서서성백내장적작용.방법 실험연구.용아서산납제작대서백내장모형,분위3조,매조9지,분별용50 g/L、20 g/L적L-기태적안액급생리염수적안3주,관찰대서정상체변화정황.장대서백내장정상체체외배양,분별가입1.00、0.10급0.01 g/L적L-기태무혈청영양액,배양1주,관찰대서정상체변화.재장배양적대서백내장정상체진행쌍향전영실험.채용방차분석대각조간적균수진행비교.결과 대서용약1주,50g/L、20g/L적L-기태조화대조조적정상체병변도수분별위2.22±0.65、2.39±0.98급2.83±0.38;50 g/L적L-기태조병변경우대조조,차이유통계학의의(P=0.013).용약2주화3주,3조간정상체병변차이무통계학의의(P>0.05).대서자내장정상체배양1주,1.00、0.10급0.01 g/L적L-기태조정상체병변여대조조차이무통계학의의(P>0.05).쌍향전영결과현시:약물조단백총수위182,대조조위161,약물조고상대분자질량단백수량감소,저상대분자질량단백수량증다.결론 50 g/L적L-기태가억제대서백내장조기병변적발전;기태재체외가사대서백내장품상체단백발생변화,대백내장정상체병변정도작용불명현.
Objective To explore the effect of L-canosine in preventing and treating rat cataract induced by sodium selenite. Methods This was an experimental study. Cataract was induced in the rats by sodium selenite. Rats were divided into 3 groups and each group has 3 ones. L-canosine eye drops (50 g/L or 20 g/L) and 0.9% normal saline were respectively instilled to the rat eye for 3 weeks and examined. L-canosine was added to the medium of cultured rat cataract lens at 1.00, 0.10 and 0.01 g/L for 1 week and then examined. Cataract lens were studied by using two-dimensional eletrophoresis. Results One week after instillation of L-canosine, the scores of rat cataract were 2.22±0.65, 2.39±0.98 and 2.83±0.38 in 50 g/L, 20 g/L L-canosine and control groups, respectively. The lesion in 50 g/l, L-canosine group was lighter than that of the control group. There was significant difference between these two groups (P=0.013). On the 2nd and 3rd weeks, there was no difference among these three groups. After 1 week for culturing cataract lens, there was no difference between these three groups. The results of two-dimensional eletrophoresis showed that the protein number was 182 and 161 in the L-carnosine and control groups, respectively. High molecular weight protein decreased and low molecular weight protein increased in L-carnosine group. Conclusions L-carnosine at 50 g/L could restrain the development of early stage rat cataract. L-camosine could modulate the rat lens protein in vitro, but could not affect lens cataract scores.
