CAJ | 학술논문

为从鱼油中分离高纯度二十碳五烯酸（EPA）和二十二碳六烯酸（DHA），根据分子间的作用力与分子结构的关系，以及高效液相色谱（HPLC）分离物质的原理，利用二乙烯苯交联的聚甲基丙烯酸甲酯（PMDVB）树脂微球作为HPLC固定相，选择适当的流动相，从鱼油中分离EPA，DHA，得到较好的分离效果。作者探索了微悬浮法合成PMDVB树脂的合成条件，并研究了交联度的大小和致孔剂组成对分离效果的影响。交联度为35%，并具有特定孔结构的PMDVB树脂微球作为HPLC固定相，具有较高的分离度。
위종어유중분리고순도이십탄오희산（EPA）화이십이탄륙희산（DHA），근거분자간적작용력여분자결구적관계，이급고효액상색보（HPLC）분리물질적원리，이용이을희분교련적취갑기병희산갑지（PMDVB）수지미구작위HPLC고정상，선택괄당적류동상，종어유중분리EPA，DHA，득도교호적분리효과。작자탐색료미현부법합성PMDVB수지적합성조건，병연구료교련도적대소화치공제조성대분리효과적영향。교련도위35%，병구유특정공결구적PMDVB수지미구작위HPLC고정상，구유교고적분리도。
Through analysis of the interaction between molecules, porouspolymethacrylatedivinybenzene (PMDVB) resin was applied as the high-performance liquid chromatography (HPLC) stationary phase for separating EPA and DHA from fish oil. Satisfactory results were obtained with proper selection of the liquid phase. The analysis was used to optimize the conditions for the microsuspens method for preparation of PMDVB and to study the effects of different rates of cross-linking and pore-induced reagents on separation results. A cross-linking rate of 35% with the special porous resin applied as the HPLC stationary phase produced a satisfactory separation rate.