西北植物学报
西北植物學報
서북식물학보
ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA
2006年
5期
871-877
,共7页
阳文龙%刘敬梅%刘强%公衍道%赵南明
暘文龍%劉敬梅%劉彊%公衍道%趙南明
양문룡%류경매%류강%공연도%조남명
非生物逆境胁迫%ABA%丝裂原活化蛋白激酶%高羊茅
非生物逆境脅迫%ABA%絲裂原活化蛋白激酶%高羊茅
비생물역경협박%ABA%사렬원활화단백격매%고양모
abiotic stress%ABA%mitogen-activated kinase%Festuca arundinacea Schreb
丝裂原活化蛋白激酶(MAPK)是生物体内信号转导的重要组分,与生长、发育和逆境胁迫反应密切相关.为了研究草坪草对非生物逆境胁迫反应的分子机理,利用同源基因克隆法从4 C低温诱导的草坪草高羊茅(Festuca arundinacea Schreb.)幼苗cDNA文库中分离得到一个MAPK的cDNA即FaMAPK1,FaMAPK1编码369个氨基酸残基的蛋白激酶,该蛋白激酶具有TEY的磷酸化基序.据推测的氨基酸序列的BLAST同源性分析表明,FaMAPK1蛋白与水稻OsMAPK4蛋白的一致性为91.1%.Northern杂交检测FaMAPK1基因对逆境胁迫反应的结果表明冷(4 C)处理对根中FaMAPK1基因的表达没有明显影响,但诱导叶中FaMAPK1上调表达.而且低温(4 C)、高盐(250 mmol/L NaCl)、干旱和100μmol/L ABA都诱导叶中FaMAPK1上调表达,表明FaMAPK1蛋白可能在高羊茅对非生物逆境胁迫的反应中起重要作用.
絲裂原活化蛋白激酶(MAPK)是生物體內信號轉導的重要組分,與生長、髮育和逆境脅迫反應密切相關.為瞭研究草坪草對非生物逆境脅迫反應的分子機理,利用同源基因剋隆法從4 C低溫誘導的草坪草高羊茅(Festuca arundinacea Schreb.)幼苗cDNA文庫中分離得到一箇MAPK的cDNA即FaMAPK1,FaMAPK1編碼369箇氨基痠殘基的蛋白激酶,該蛋白激酶具有TEY的燐痠化基序.據推測的氨基痠序列的BLAST同源性分析錶明,FaMAPK1蛋白與水稻OsMAPK4蛋白的一緻性為91.1%.Northern雜交檢測FaMAPK1基因對逆境脅迫反應的結果錶明冷(4 C)處理對根中FaMAPK1基因的錶達沒有明顯影響,但誘導葉中FaMAPK1上調錶達.而且低溫(4 C)、高鹽(250 mmol/L NaCl)、榦旱和100μmol/L ABA都誘導葉中FaMAPK1上調錶達,錶明FaMAPK1蛋白可能在高羊茅對非生物逆境脅迫的反應中起重要作用.
사렬원활화단백격매(MAPK)시생물체내신호전도적중요조분,여생장、발육화역경협박반응밀절상관.위료연구초평초대비생물역경협박반응적분자궤리,이용동원기인극륭법종4 C저온유도적초평초고양모(Festuca arundinacea Schreb.)유묘cDNA문고중분리득도일개MAPK적cDNA즉FaMAPK1,FaMAPK1편마369개안기산잔기적단백격매,해단백격매구유TEY적린산화기서.거추측적안기산서렬적BLAST동원성분석표명,FaMAPK1단백여수도OsMAPK4단백적일치성위91.1%.Northern잡교검측FaMAPK1기인대역경협박반응적결과표명랭(4 C)처리대근중FaMAPK1기인적표체몰유명현영향,단유도협중FaMAPK1상조표체.이차저온(4 C)、고염(250 mmol/L NaCl)、간한화100μmol/L ABA도유도협중FaMAPK1상조표체,표명FaMAPK1단백가능재고양모대비생물역경협박적반응중기중요작용.
Mitogen-activated kinase (MAPK) is the important component of the signal transmission in living things and closely related to growth,development and responses to adverse environments. In order to explore the molecular mechanism of turf-grass response (Festuca arundinacea Schreb. ) to abiotic stress,homologous gene cloning was adopted to isolate cDNA of a MAPK, FaMAPK1, from the turf-grass seedlings induced at 4 C. FaMAPK1 encoded a protein kinase with 369 amino acid residuals,which had phosphorylation activation motif of TEY. The amino acid sequencing by BLAST showed that the amino acid sequence of FaMAPK1 was 91.1% identical with that of OsMAPK4 in rice. The responses of FaMAPK1 to adverse environmental stress were tested by Northern blotting to show that chilling (at 4 C )did not exert influence on FaMAPK1 gene expression in the roots but FaMAPK1 gene expression was upregulated in the induced leaves. Furthermore, low temperature (at 4C), high salt concentration (250 mmol/L NaC1),drought, 100 μmol/L ABA could led to a up-regulated expression of FaMAPK1,and this indicated that FaMAPK1 probably played an important role in the responses of F. arundinacea to abiotic stresses.
