中国抗生素杂志
中國抗生素雜誌
중국항생소잡지
CHINESE JOURNAL OF ANTIBIOTICS
2010年
2期
96-99,155
,共5页
张鹏伟%储消和%郭美锦%储炬%庄英萍%张嗣良
張鵬偉%儲消和%郭美錦%儲炬%莊英萍%張嗣良
장붕위%저소화%곽미금%저거%장영평%장사량
盐霉素%响应面设计%培养条件优化
鹽黴素%響應麵設計%培養條件優化
염매소%향응면설계%배양조건우화
Salinomycin%Response surface methodology%Fermentation conditions optimization
利用SAS,Minitab和Design-Expert软件,采用Placket-Burman设计和中心组合设计对盐霉素的发酵条件进行优化.并运用响应面方法对盐霉素发酵条件进行进一步的分析,根据建立的多元二次方程确定其最佳水平,得到最佳的培养基:糊精4.5%,玉米浆0.6%,黄豆粉0.3%,氯化钾0.3%,硝酸铵0.3%,磷酸氧二钾0.04%,豆油10%,碳酸钙0.1%.培养条件为:接种量10%,pH 7.0,摇床转速为250r/min,培养周期10d.在此最优化条件下得到盐霉素发酵单位比优化前的产量提高了44%.
利用SAS,Minitab和Design-Expert軟件,採用Placket-Burman設計和中心組閤設計對鹽黴素的髮酵條件進行優化.併運用響應麵方法對鹽黴素髮酵條件進行進一步的分析,根據建立的多元二次方程確定其最佳水平,得到最佳的培養基:糊精4.5%,玉米漿0.6%,黃豆粉0.3%,氯化鉀0.3%,硝痠銨0.3%,燐痠氧二鉀0.04%,豆油10%,碳痠鈣0.1%.培養條件為:接種量10%,pH 7.0,搖床轉速為250r/min,培養週期10d.在此最優化條件下得到鹽黴素髮酵單位比優化前的產量提高瞭44%.
이용SAS,Minitab화Design-Expert연건,채용Placket-Burman설계화중심조합설계대염매소적발효조건진행우화.병운용향응면방법대염매소발효조건진행진일보적분석,근거건립적다원이차방정학정기최가수평,득도최가적배양기:호정4.5%,옥미장0.6%,황두분0.3%,록화갑0.3%,초산안0.3%,린산양이갑0.04%,두유10%,탄산개0.1%.배양조건위:접충량10%,pH 7.0,요상전속위250r/min,배양주기10d.재차최우화조건하득도염매소발효단위비우화전적산량제고료44%.
The fermentation condition of salinomycin prodction was optimized through Plackeet-Burman experimental design and central composite design(CCD)with the aid of SAS,Minitab and Design-Expert softwares.The levels of fermentation condition were further screened and identified by response surface methodolgy.From the resulting of multi-quadratic equation,the optimized levels of influencing factors could be estimated.The optimized medium was obtained as follows:dextrin 4.5%,corn steep liquor 0.6%,soybeen meal 0.3%,KCI 0.3%,NH4NO3 0.3%,KH_2PO_4 0.04%,oil 10%,CaCO_3 0.1%and initial pH 7.0.The optimized culture conditions were also established:shaker speed 250r/min,inoculum size 10%V/V,and cultivation duration for 10 days.The salinomycin production titer under this developed conditions in shaking flask culture was increased by 44%compared with that obtained at the unoptimized conditions,indicating the newly developed fermentation conditions for salinomycin production has a promising application in the industrial production.