CAJ | 학술논문

利用SAS,Minitab和Design-Expert软件,采用Placket-Burman设计和中心组合设计对盐霉素的发酵条件进行优化.并运用响应面方法对盐霉素发酵条件进行进一步的分析,根据建立的多元二次方程确定其最佳水平,得到最佳的培养基:糊精4.5%,玉米浆0.6%,黄豆粉0.3%,氯化钾0.3%,硝酸铵0.3%,磷酸氧二钾0.04%,豆油10%,碳酸钙0.1%.培养条件为:接种量10%,pH 7.0,摇床转速为250r/min,培养周期10d.在此最优化条件下得到盐霉素发酵单位比优化前的产量提高了44%.
이용SAS,Minitab화Design-Expert연건,채용Placket-Burman설계화중심조합설계대염매소적발효조건진행우화.병운용향응면방법대염매소발효조건진행진일보적분석,근거건립적다원이차방정학정기최가수평,득도최가적배양기:호정4.5%,옥미장0.6%,황두분0.3%,록화갑0.3%,초산안0.3%,린산양이갑0.04%,두유10%,탄산개0.1%.배양조건위:접충량10%,pH 7.0,요상전속위250r/min,배양주기10d.재차최우화조건하득도염매소발효단위비우화전적산량제고료44%.
The fermentation condition of salinomycin prodction was optimized through Plackeet-Burman experimental design and central composite design(CCD)with the aid of SAS,Minitab and Design-Expert softwares.The levels of fermentation condition were further screened and identified by response surface methodolgy.From the resulting of multi-quadratic equation,the optimized levels of influencing factors could be estimated.The optimized medium was obtained as follows:dextrin 4.5%,corn steep liquor 0.6%,soybeen meal 0.3%,KCI 0.3%,NH4NO3 0.3%,KH_2PO_4 0.04%,oil 10%,CaCO_3 0.1%and initial pH 7.0.The optimized culture conditions were also established:shaker speed 250r/min,inoculum size 10%V/V,and cultivation duration for 10 days.The salinomycin production titer under this developed conditions in shaking flask culture was increased by 44%compared with that obtained at the unoptimized conditions,indicating the newly developed fermentation conditions for salinomycin production has a promising application in the industrial production.