CAJ | 학술논문

目的观察缺氧诱导因子1α(HIF-1α)和碱性成纤维细胞生长因子(BFGF)在大肠腺癌组织中的表达,探讨其表达与大肠腺癌生物学行为关系.方法采用免疫组织化学染色检测手术切除的大肠腺癌组织(n=60)、癌旁组织(n=60)和正常大肠黏膜组织(n=20)中HIF-1α和BFGF表达.结果 HIF-1α和BFGF在大肠腺癌组织中表达阳性率[61.7%(37/60),65.0%(39/60)]较癌旁组织[10.0%(6/60),13.3%(8/60)]明显升高(P<0.05),正常肠黏膜组织中未检出HIF-1α和BFGF表达;HIF-1α和BFGF在大肠腺癌组织中表达与患者性别、年龄、肿瘤大小、肿瘤位置和分化程度无关(P>0.05),与肿瘤Dukes分期有关(P<0.05):HIF-1α和BFGF在Dukes A、B期(n=36)表达阳性率分别为47.2%和52.7%,在Dukes C、D期(n=24)分别为83.3%和83.3%(P<0.05);大肠腺癌组织中HIF-1α和BFGF表达具有相关性(r=0.4276,P<0.001).结论大肠腺癌组织中HIF-1α和BFGF表达水平上调,可能与大肠腺癌的预后有关;HIF-1α和BFGF有协同作用,共同促进大肠腺癌的发生.
목적 관찰결양유도인자1α(HIF-1α)화감성성섬유세포생장인자(BFGF)재대장선암조직중적표체,탐토기표체여대장선암생물학행위관계.방법 채용면역조직화학염색검측수술절제적대장선암조직(n=60)、암방조직(n=60)화정상대장점막조직(n=20)중HIF-1α화BFGF표체.결과 HIF-1α화BFGF재대장선암조직중표체양성솔[61.7%(37/60),65.0%(39/60)]교암방조직[10.0%(6/60),13.3%(8/60)]명현승고(P<0.05),정상장점막조직중미검출HIF-1α화BFGF표체;HIF-1α화BFGF재대장선암조직중표체여환자성별、년령、종류대소、종류위치화분화정도무관(P>0.05),여종류Dukes분기유관(P<0.05):HIF-1α화BFGF재Dukes A、B기(n=36)표체양성솔분별위47.2%화52.7%,재Dukes C、D기(n=24)분별위83.3%화83.3%(P<0.05);대장선암조직중HIF-1α화BFGF표체구유상관성(r=0.4276,P<0.001).결론 대장선암조직중HIF-1α화BFGF표체수평상조,가능여대장선암적예후유관;HIF-1α화BFGF유협동작용,공동촉진대장선암적발생.
Objective To observe expression of hypoxia inducible factor-1α(HIF-1α)and basic fibroblast growth factor(BFGF)in the tissues of colorectal adenoearcinoma and analyze the relationship between expression of the two factors and biological behavior of colorectal adenocarcinoma.Methods The samples of colorectal adenocarcinoma(n=60)and para-adenocarcinoma(n=60)were taken from surgical resection patients and normal colorectal tissues (n=20) from patients with irritable bowel syndrome.The expression of HIF-1α and BFGF were detected by immunohistochemical staining (SP method).Results Positive rates of HIF-1αand BFGF in colorectal adenocarcinoma tissues were 61.7% and 65.0%,and positive rates of HIF-1α and BFGF in para-adenocarcinoma tissues were 10.0%and 13.3%(P<0.05,respectively);HIF-1αand BFGF were not detected in normal intestinal mucosa.There was no significant correlation with HIF-1α and BFGF expression in colorectal adenocarcinoma tissues to the sexuality,age,tumor size,tumorposition and differentiation(P>0.05).A significant correlation between expression of the two factors and Dukes stage was observed (P<0.05).Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissuesof Dukes A and B stage were 47.2%and 52.7%,respectively.Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues of Dukes C and D stage were 83.3%and 83.3%.respectively.There was a positive correlation between expression of the two factors and carcinogenesis of colorectal adenocarcinoma(r=0.4276.P<0.001).Conclusion The results showed that the enhanced expression of HIF-1 α and BFGF incolorectal adenocarcinoma tissues may be associated with the prognosis of the patients with colorectal adenocarcinoma,and HIF-1α and BFGF may participate synergistically in the carcinogenesis of colorectal adenocarcinoma.