中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2010年
6期
692-695
,共4页
陈遐林%纪蓉%曹建平%朱巍%樊赛军%王建芳
陳遐林%紀蓉%曹建平%硃巍%樊賽軍%王建芳
진하림%기용%조건평%주외%번새군%왕건방
p53%细胞周期%HeLa细胞%SiHa细胞%二氢青蒿素
p53%細胞週期%HeLa細胞%SiHa細胞%二氫青蒿素
p53%세포주기%HeLa세포%SiHa세포%이경청호소
p53%Cell cycle%Human HeLa cells%Human SiHa cells%Dihydroartemisinin
目的 观察二氢青蒿素及X射线对肿瘤细胞周期的影响,并研究其具体作用机制.方法 选用已知p53突变的人宫颈癌HeLa细胞,并以p53功能正常的人宫颈癌SiHa细胞作为对照.采用流式细胞术分析X射线(6 Gy)、二氢青蒿素(20及100 μmol/L)对两种细胞的细胞周期的影响;应用蛋白印迹法(Western blot)检测细胞周期相关蛋白表达量的变化.结果 X射线照射明显导致HeLa细胞G2期阻滞,照射后G2期细胞比例由14.45%上升至73.58%,在二氢青蒿素联合照射作用后,HeLa细胞G2期细胞比例由单纯照射组的73.58%降至48.31%;而对照组SiHa细胞G2期变化不明显.在单纯照射组,随着细胞G2期阻滞的增加,HeLa细胞中Weel蛋白表达量增加,Cyclin B1蛋白表达量降低,而在二氢青蒿素联合照射作用后,细胞内Weel蛋白表达量较单纯照射组减少,Cyclin B1蛋白表达量较单纯照射组增高,与该药能去除电离辐射导致细胞G2期阻滞过程相一致.结论 对于p53突变的人宫颈癌HeLa细胞,二氢青蒿素能抑制辐射所引起的细胞G2期阻滞,其机理可能与细胞周期调控蛋白Wee1、Cyclin B1表达变化有关;对p53功能正常的SiHa细胞,辐射主要引起细胞G1期阻滞,故二氢青蒿素对其周期的影响作用不明显.
目的 觀察二氫青蒿素及X射線對腫瘤細胞週期的影響,併研究其具體作用機製.方法 選用已知p53突變的人宮頸癌HeLa細胞,併以p53功能正常的人宮頸癌SiHa細胞作為對照.採用流式細胞術分析X射線(6 Gy)、二氫青蒿素(20及100 μmol/L)對兩種細胞的細胞週期的影響;應用蛋白印跡法(Western blot)檢測細胞週期相關蛋白錶達量的變化.結果 X射線照射明顯導緻HeLa細胞G2期阻滯,照射後G2期細胞比例由14.45%上升至73.58%,在二氫青蒿素聯閤照射作用後,HeLa細胞G2期細胞比例由單純照射組的73.58%降至48.31%;而對照組SiHa細胞G2期變化不明顯.在單純照射組,隨著細胞G2期阻滯的增加,HeLa細胞中Weel蛋白錶達量增加,Cyclin B1蛋白錶達量降低,而在二氫青蒿素聯閤照射作用後,細胞內Weel蛋白錶達量較單純照射組減少,Cyclin B1蛋白錶達量較單純照射組增高,與該藥能去除電離輻射導緻細胞G2期阻滯過程相一緻.結論 對于p53突變的人宮頸癌HeLa細胞,二氫青蒿素能抑製輻射所引起的細胞G2期阻滯,其機理可能與細胞週期調控蛋白Wee1、Cyclin B1錶達變化有關;對p53功能正常的SiHa細胞,輻射主要引起細胞G1期阻滯,故二氫青蒿素對其週期的影響作用不明顯.
목적 관찰이경청호소급X사선대종류세포주기적영향,병연구기구체작용궤제.방법 선용이지p53돌변적인궁경암HeLa세포,병이p53공능정상적인궁경암SiHa세포작위대조.채용류식세포술분석X사선(6 Gy)、이경청호소(20급100 μmol/L)대량충세포적세포주기적영향;응용단백인적법(Western blot)검측세포주기상관단백표체량적변화.결과 X사선조사명현도치HeLa세포G2기조체,조사후G2기세포비례유14.45%상승지73.58%,재이경청호소연합조사작용후,HeLa세포G2기세포비례유단순조사조적73.58%강지48.31%;이대조조SiHa세포G2기변화불명현.재단순조사조,수착세포G2기조체적증가,HeLa세포중Weel단백표체량증가,Cyclin B1단백표체량강저,이재이경청호소연합조사작용후,세포내Weel단백표체량교단순조사조감소,Cyclin B1단백표체량교단순조사조증고,여해약능거제전리복사도치세포G2기조체과정상일치.결론 대우p53돌변적인궁경암HeLa세포,이경청호소능억제복사소인기적세포G2기조체,기궤리가능여세포주기조공단백Wee1、Cyclin B1표체변화유관;대p53공능정상적SiHa세포,복사주요인기세포G1기조체,고이경청호소대기주기적영향작용불명현.
Objective To observe the changes of cell cycle on cancer cells after dihydroartemisinin and X-ray irradiation. Methods Human HeLa cells of cervical cancer with p53 mutation was used and human SiHa cells of cervical cancer with wild p53 was used as control. Flow cytometry was used to detect the effect of dihydroartemisinin (20 and 100 μmol/L) and irradiation (6 Gy)on cell cycle. Western blot was used to measure the levels of cell cycle protein. Results G2 arrest was observed in irradiated HeLa cells, which the proportion of cells in G2 phase was increased from 14.45% to 73. 58% after 6 Gy X-ray irradiation, but it was abrogated by dihydroartemisinin from 73. 58% to 48.31%in HeLa cells, and it had no change on the SiHa cells. The elevated Weel protein and the lowered Cyclin B1 protein were observed with the G2 arrest severity. The expression of radiation-induced Weel protein was suppressed and the Cyclin B1 protein was increased after dihydroartemisinin treatment, which was in accordance with the abrogation of radiation-induced G2 delay. Conclusions The main effect of irradiation on cell cycle of p53 mutated HeLa cells is G2 arrest. Dihydroartemisinin could abrogate it, which is associated with the changes of Weel protein and Cyclin B1 protein. In Siha cells, the main effect of irradiation on cell cycle is G1 arrest, and dihydroartemisinin has no effect on it.
