CAJ | 학술논문

目的研究晕痣自发性消退早期及晚期皮损区炎细胞表型、数目及其分布特点,探讨晕痣自发性消退的免疫机制.方法应用免疫组化方法对晕痣皮损区、非皮损区及正常对照皮肤进行CD3、CD4、CD8、CD20、CD1a、CD56、CD68染色,用计算机图像分析系统(Image-pro PLUS 6.0)观察及定量分析.结果晕痣早期及晚期皮损区CD4、CD8、CD20、CD1a阳性细胞数均明显高于非皮损区及正常对照皮肤(P＜0.01),皮损区CD1a、CD68阳性细胞直径明显大于非皮损区及正常对照皮肤(P＜0.01).消退晚期CD8与CD4阳性细胞数目比值(2.05∶1)高于消退早期(1.82∶1),CD8阳性细胞在痣细胞巢内大量浸润.结论 CD4、CD8、CD20、CD1a、CD56、CD68阳性细胞均参与晕痣自发性消退,CD8阳性T细胞在晕痣消退中起主要作用.
목적 연구훈지자발성소퇴조기급만기피손구염세포표형、수목급기분포특점,탐토훈지자발성소퇴적면역궤제.방법 응용면역조화방법 대훈지피손구、비피손구급정상대조피부진행CD3、CD4、CD8、CD20、CD1a、CD56、CD68염색,용계산궤도상분석계통(Image-pro PLUS 6.0)관찰급정량분석.결과 훈지조기급만기피손구CD4、CD8、CD20、CD1a양성세포수균명현고우비피손구급정상대조피부(P＜0.01),피손구CD1a、CD68양성세포직경명현대우비피손구급정상대조피부(P＜0.01).소퇴만기CD8여CD4양성세포수목비치(2.05∶1)고우소퇴조기(1.82∶1),CD8양성세포재지세포소내대량침윤.결론 CD4、CD8、CD20、CD1a、CD56、CD68양성세포균삼여훈지자발성소퇴,CD8양성T세포재훈지소퇴중기주요작용.
Objective To investigate the phenotype,number and distribution of inflammatory cells in early and late stages of spontaneous regression of halo nevi,and to elucidate the immunological mechanisms for spontaneous regression of these nevi.Methods Halo nevi,their surrounding non-lesional skin,and normal control skin were examined by immunohistochemical staining with monoclonal antibodies to CD3,CD4,CD8,CD20,CD1a,CD56 and CD68.Staining results were observed and analyzed by the computer image analysis system,image-pro plus 6.0.Results The number of CD4+,CD8+,CD20+,CD1a+cells,along with the diameter of CD1a+and CD68+ cells was significantly increased in the lesions of early and late stage of spontaneous regression of halo nevi than in non-lesional skin and normal control skin(both P＜0.01).The ratio of CD8+/CD4+ cells in the lesions of late stage of spontaneous regression was also higher than that in the lesions of early stage (2.05∶1 VS 1.82∶1).A massive infiltrate of CD8+ cells was observed in the nests of nevus cells.Conclusions CD4,CD8,CD20,CD1a,CD56 and CD68 positive cells are all involved in the spontaneous regression of halo nevi,and CD8+ cells may play a predominant role in this process.