中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2011年
22期
7-9
,共3页
赵倩%周勇%王奕%高清歌%陈建杨%姜晓艳
趙倩%週勇%王奕%高清歌%陳建楊%薑曉豔
조천%주용%왕혁%고청가%진건양%강효염
格雷夫斯病%桥本病%自身免疫%连接蛋白类%逆转录聚合酶链反应
格雷伕斯病%橋本病%自身免疫%連接蛋白類%逆轉錄聚閤酶鏈反應
격뢰부사병%교본병%자신면역%련접단백류%역전록취합매련반응
Graves disease%Hashimoto disease%Autoimmunity%Connexins%Reverse transcriptase polymerase chain reaction
目的 研究自身免疫性甲状腺疾病(AITD)患者甲状腺组织中缝隙连接蛋白(Cx)43、Cx26基因表达的变化.方法 外科手术收集76例甲状腺组织,均经病理诊断.其中Graves病(CD)30例(GD组),桥本甲状腺炎(HT)30例(HT组),甲状腺腺瘤旁正常甲状腺组织16例(正常组).应用反转录(RT)-聚合酶链反应(PCR)技术,以GAPDH为内参照分析三组甲状腺组织中Cx43、Cx26mRNA表达的差异.结果 正常及AITD甲状腺组织中均表达Cx43及Cx26 mRNA;Cx43及Cx26mRNA在GD组甲状腺组织中的表达明显高于正常组(0.9307±0.0716比0.5938±0.0484,0.7371±0.0463比0.3431±0.0399)(P＜0.01),在HT组中的表达明显低于正常组(0.3581±0.0458比0.5938±0.0484,0.3150±0.0218比0.3431±0.0399)(P＜0.01或＜0.05).结论 Cx43、Cx26 mRNA在人甲状腺组织中有所表达,且其表达在AITD患者甲状腺组织中有明显变化,提示缝隙连接与AITD发生有关.
目的 研究自身免疫性甲狀腺疾病(AITD)患者甲狀腺組織中縫隙連接蛋白(Cx)43、Cx26基因錶達的變化.方法 外科手術收集76例甲狀腺組織,均經病理診斷.其中Graves病(CD)30例(GD組),橋本甲狀腺炎(HT)30例(HT組),甲狀腺腺瘤徬正常甲狀腺組織16例(正常組).應用反轉錄(RT)-聚閤酶鏈反應(PCR)技術,以GAPDH為內參照分析三組甲狀腺組織中Cx43、Cx26mRNA錶達的差異.結果 正常及AITD甲狀腺組織中均錶達Cx43及Cx26 mRNA;Cx43及Cx26mRNA在GD組甲狀腺組織中的錶達明顯高于正常組(0.9307±0.0716比0.5938±0.0484,0.7371±0.0463比0.3431±0.0399)(P＜0.01),在HT組中的錶達明顯低于正常組(0.3581±0.0458比0.5938±0.0484,0.3150±0.0218比0.3431±0.0399)(P＜0.01或＜0.05).結論 Cx43、Cx26 mRNA在人甲狀腺組織中有所錶達,且其錶達在AITD患者甲狀腺組織中有明顯變化,提示縫隙連接與AITD髮生有關.
목적 연구자신면역성갑상선질병(AITD)환자갑상선조직중봉극련접단백(Cx)43、Cx26기인표체적변화.방법 외과수술수집76례갑상선조직,균경병리진단.기중Graves병(CD)30례(GD조),교본갑상선염(HT)30례(HT조),갑상선선류방정상갑상선조직16례(정상조).응용반전록(RT)-취합매련반응(PCR)기술,이GAPDH위내삼조분석삼조갑상선조직중Cx43、Cx26mRNA표체적차이.결과 정상급AITD갑상선조직중균표체Cx43급Cx26 mRNA;Cx43급Cx26mRNA재GD조갑상선조직중적표체명현고우정상조(0.9307±0.0716비0.5938±0.0484,0.7371±0.0463비0.3431±0.0399)(P＜0.01),재HT조중적표체명현저우정상조(0.3581±0.0458비0.5938±0.0484,0.3150±0.0218비0.3431±0.0399)(P＜0.01혹＜0.05).결론 Cx43、Cx26 mRNA재인갑상선조직중유소표체,차기표체재AITD환자갑상선조직중유명현변화,제시봉극련접여AITD발생유관.
Objective To investigate the expression of connexin (Cx) 43, Cx26 gene in the thyroid tissue in patients with autoimmune thyroid diseases (AITD).Methods The thyroid tissue specimens of 76 cases were selected, including 30 cases of Graves disease (GD group), 30 cases of Hashimoto thyroiditis( HT group ), and 16 cases of normal tissues from thyroid adenoma (control group), which were all confirmed by pathologic diagnosis.The expression of Cx43 and Cx26 mRNA among three groups was determined by reverse transcriptase polymerase chain reaction (RT-PCR), and GAPDH,which was used as the internal control.Results The staining of Cx43 and Cx26 mRNA in all the cases from human thyroid tissue were strongly positive.The expression levels of Cx43,Cx26 mRNA in GD group were significantly higher than those in control group (0.9307 ± 0.0716 vs.0.5938 ± 0.0484 and 0.7371 ± 0.0463 vs.0.3431 ± 0.0399 ) (P ＜ 0.01 ).And the expression levels of Cx43,Cx26 mRNA in HT group were significantly lower than those in control group (0.3581 ±0.0458 vs.0.5938 ±0.0484 and 0.3150 ±0.0218 vs.0.3431 ±0.0399)(P＜0.01 or ＜0.05).Conclusion The Cx43 and Cx26 mRNA expression in the human thyroid tissue and a remarkable deferral expressions of Cx43 and Cx26 mRNA in three groups of AITD suggests that gap junction is associated with AITD.
