中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2009年
4期
398-400
,共3页
高怡%韩光%梁江%田凤洁%裴秋玲
高怡%韓光%樑江%田鳳潔%裴鞦玲
고이%한광%량강%전봉길%배추령
砷中毒%T淋巴细胞亚群%免疫
砷中毒%T淋巴細胞亞群%免疫
신중독%T림파세포아군%면역
Arsenic poisoning%T-lymphocyte subsets%Immunity
目的 探讨饮水型砷暴露人群外周血T淋巴细胞免疫功能的改变及意义.方法 2006年,在山西省朔州市朔城区砷中毒病区选择有饮水砷接触史,有慢性砷中毒症状的53例(男性26例、女性27例)患者作为病例组,以当地无慢性砷中毒症状者48例(男性23例,女性25例)作为对照组.采集外周血,采用碱性磷酸酶标记链霉卵白素法(SAP法)进行T淋巴细胞亚群CD3+、CD4+、CD8+细胞检测.结果 病例组外周血CD3+、CD4+和CD4+/CD8+[(41.89±11.58)%、(25.60±9.05)%、1.02±0.41]低于对照组[(68.38±7.23)%、(39.17±4.28)%、1.69±0.56,t=13.61、18.72、14.79,P均<0.05];病例组CD8+[(25.30±6.85)%]与对照组[(23.54±8.35)%]比较,差异无统计学意义(t=3.07,P>0.05).多因素线性逐步回归分析结果表明,CD4+、CD8+与性别有关(t=-3.05、-4.30,P均<0.05);病例组CD3+、CD4+、CD8+、CD4+/CD8+,男性分别为(40.65±10.06)%、(24.48±6.29)%、(24.52±8.16)%、0.98±0.25,与女性[(43.07±12.96)%、(26.77±3.12)%、(26.50±9.32)%、1.07±0.41]比较,差异均无统计学意义(t=-0.76、3.05、0.30、2.10,P均>0.05).结论 饮水型慢性砷中毒患者T淋巴细胞免疫功能受到明显抑制作用,检测患者外周血T细胞亚群表达对评估患者的细胞免疫功能并为提供慢性砷中毒早期诊断指标具有重要意义.
目的 探討飲水型砷暴露人群外週血T淋巴細胞免疫功能的改變及意義.方法 2006年,在山西省朔州市朔城區砷中毒病區選擇有飲水砷接觸史,有慢性砷中毒癥狀的53例(男性26例、女性27例)患者作為病例組,以噹地無慢性砷中毒癥狀者48例(男性23例,女性25例)作為對照組.採集外週血,採用堿性燐痠酶標記鏈黴卵白素法(SAP法)進行T淋巴細胞亞群CD3+、CD4+、CD8+細胞檢測.結果 病例組外週血CD3+、CD4+和CD4+/CD8+[(41.89±11.58)%、(25.60±9.05)%、1.02±0.41]低于對照組[(68.38±7.23)%、(39.17±4.28)%、1.69±0.56,t=13.61、18.72、14.79,P均<0.05];病例組CD8+[(25.30±6.85)%]與對照組[(23.54±8.35)%]比較,差異無統計學意義(t=3.07,P>0.05).多因素線性逐步迴歸分析結果錶明,CD4+、CD8+與性彆有關(t=-3.05、-4.30,P均<0.05);病例組CD3+、CD4+、CD8+、CD4+/CD8+,男性分彆為(40.65±10.06)%、(24.48±6.29)%、(24.52±8.16)%、0.98±0.25,與女性[(43.07±12.96)%、(26.77±3.12)%、(26.50±9.32)%、1.07±0.41]比較,差異均無統計學意義(t=-0.76、3.05、0.30、2.10,P均>0.05).結論 飲水型慢性砷中毒患者T淋巴細胞免疫功能受到明顯抑製作用,檢測患者外週血T細胞亞群錶達對評估患者的細胞免疫功能併為提供慢性砷中毒早期診斷指標具有重要意義.
목적 탐토음수형신폭로인군외주혈T림파세포면역공능적개변급의의.방법 2006년,재산서성삭주시삭성구신중독병구선택유음수신접촉사,유만성신중독증상적53례(남성26례、녀성27례)환자작위병례조,이당지무만성신중독증상자48례(남성23례,녀성25례)작위대조조.채집외주혈,채용감성린산매표기련매란백소법(SAP법)진행T림파세포아군CD3+、CD4+、CD8+세포검측.결과 병례조외주혈CD3+、CD4+화CD4+/CD8+[(41.89±11.58)%、(25.60±9.05)%、1.02±0.41]저우대조조[(68.38±7.23)%、(39.17±4.28)%、1.69±0.56,t=13.61、18.72、14.79,P균<0.05];병례조CD8+[(25.30±6.85)%]여대조조[(23.54±8.35)%]비교,차이무통계학의의(t=3.07,P>0.05).다인소선성축보회귀분석결과표명,CD4+、CD8+여성별유관(t=-3.05、-4.30,P균<0.05);병례조CD3+、CD4+、CD8+、CD4+/CD8+,남성분별위(40.65±10.06)%、(24.48±6.29)%、(24.52±8.16)%、0.98±0.25,여녀성[(43.07±12.96)%、(26.77±3.12)%、(26.50±9.32)%、1.07±0.41]비교,차이균무통계학의의(t=-0.76、3.05、0.30、2.10,P균>0.05).결론 음수형만성신중독환자T림파세포면역공능수도명현억제작용,검측환자외주혈T세포아군표체대평고환자적세포면역공능병위제공만성신중독조기진단지표구유중요의의.
Objective To study the change and the significance of T-lymphocyte immune function in peripheral blood in population living in arsenic-contaminated area. Methods Fifty-three cases of patients with arsenism symptoms were selected into experimental group, inhabitants who had no chronic arsenism symptoms into control group in the endemic area of Shuocheng District, Shuozhou City, Shanxi Province in 2006. Vein blood samples were taken and analyzed with SAP assay to measure the percentage of CD3+ ,CD4+ and CD8+ T-cells. Results It was found that the percentage of CD3+, CD4+, and CD4+/CD8+ [(41.89 ± 11.58)%, (25.60 ± 9.05)% and 1.02 ± 0.41] in the experimental group was lower than that in the control group [(68.38 ± 7.23)%, (39.17± 4.28)% ,1.69 ± 0.56, t = 13.61,18.72,14.79, all P < 0.05], while there was no statistical differences of CD8+ [(25.30 ± 6.85)%] compared to the control group[(23.54 ± 8.35)%,t = 3.07,P > 0.05]. The gender-related effect of arsenic on CD4+ and CD8+ was found by multiple linear step regression analysis(t = - 3.05, - 4.30, all P < 0.05). In case group, there were no statistical differences in CD3+, CD4+, CD8+ and CD4+/CD8+[(40.65±10.06)%, (24.48 ± 6.29)%, (24.52 ± 8.16)%,0.98 ± 0.25] between males and females [(43.07±12.96)%, (26.77±3.12)%, (26.50 ±9.32)%, 1.07 ±0.41, t = - 0.76,3.05,0.30,2.10, all P > 0.05]. Conclusions The immune function of T-lymphocytes of patients with chronic arsenism has been suppressed. It is of active significance to detect T-lymphocyte subpopulation in peripheral vein in patients with chronic arsenism aiming at estimating the function of cell immune and providing early diagnosis index.
