CAJ | 학술논문

目的探讨原癌基因人类表皮生长因子受体-2(HER-2)和拓扑异构酶Ⅱ(TOPO-Ⅱα)在卵巢上皮癌组织中的表达及其相关性.方法采用免疫组化SP方法检测经病理确诊的58例卵巢上皮癌、20例卵巢良性上皮肿瘤及10例正常卵巢组织标本中HER-2及TOPO-Ⅱα的表达情况,并结合临床病理特征进行分析.结果 HER-2在卵巢正常组织、卵巢良性上皮性肿瘤组织中的阳性表达率分别为10.0%(1/10)、15.0%(3/20),显著低于卵巢上皮癌组织[46.6%(27/58),x2值分别为4.705、6.225,P均＜0.05];卵巢上皮癌组织中TOPO-Ⅱα的阳性表达率显著高于卵巢正常组织和卵巢良性上皮性肿瘤组织[53.4%(31/58)、10.0%(1/10)、15.0%(3/20),x2值分别为6.463、8.941,P均＜0.05];而良性肿瘤组织和正常组织间比较差异均无统计学意义(P均＞0.05).HER-2和TOPO-Ⅱα的表达与卵巢上皮癌临床分期(x2值分别为5.295、6.665)、细胞组织学分化(x2值分别为5.376、8.981)有显著相关性(P均＜0.05),而与年龄、组织学类型无相关性(P均＞0.05);卵巢上皮癌中HER-2和TOPO-Ⅱα蛋白的表达呈正相关(rs=0.324,P＜0.05).结论 HER-2在卵巢上皮癌的发生发展中起重要作用,对临床诊断及预后评估具有一定的指导意义.
목적 탐토원암기인인류표피생장인자수체-2(HER-2)화탁복이구매Ⅱ(TOPO-Ⅱα)재란소상피암조직중적표체급기상관성.방법 채용면역조화SP방법검측경병리학진적58례란소상피암、20례란소량성상피종류급10례정상란소조직표본중HER-2급TOPO-Ⅱα적표체정황,병결합림상병리특정진행분석.결과 HER-2재란소정상조직、란소량성상피성종류조직중적양성표체솔분별위10.0%(1/10)、15.0%(3/20),현저저우란소상피암조직[46.6%(27/58),x2치분별위4.705、6.225,P균＜0.05];란소상피암조직중TOPO-Ⅱα적양성표체솔현저고우란소정상조직화란소량성상피성종류조직[53.4%(31/58)、10.0%(1/10)、15.0%(3/20),x2치분별위6.463、8.941,P균＜0.05];이량성종류조직화정상조직간비교차이균무통계학의의(P균＞0.05).HER-2화TOPO-Ⅱα적표체여란소상피암림상분기(x2치분별위5.295、6.665)、세포조직학분화(x2치분별위5.376、8.981)유현저상관성(P균＜0.05),이여년령、조직학류형무상관성(P균＞0.05);란소상피암중HER-2화TOPO-Ⅱα단백적표체정정상관(rs=0.324,P＜0.05).결론 HER-2재란소상피암적발생발전중기중요작용,대림상진단급예후평고구유일정적지도의의.
Objective To observe the expression of HER-2 and TOPO-Ⅱα in ovarian epithelial cancer,analyze the correlation between their expression and provide theoretical basis for clinical diagnosis,prognosis and treatment. Methods Expression levels of HER-2 and TOPO- Ⅱα in 10 normal ovarian tissues,20 benign tumors and 58 cases of ovarian epithelial cancers were detected by immunohistochemical method, and their correlations with pathological features were analyzed. Results The positive expression rate of HER-2 in normal ovarian and benign tumor tissues were significantly lower than ovarian epithelial cancers respectively ( 10. 0% , 15.0% VS 46. 6% ;P ＜ 0. 05 ). The positive expression rate of TOPO- Ⅱα in ovarian epithelial cancers was significantly higher than normal and benign epithelial ovarian tumor tissue (53.4% vs 10. 0%, and 15.0%,Ps ＜ 0. 05 ), but we did not find significant difference in the comparison between normal and benign epithelial ovarian tumor tissue ( Ps ＞ 0. 05 ). The expression of HER-2 and TOPO- Ⅱα were significantly correlated with clinical stages, histological differentiation of tumor cells (Ps ＜ 0. 05 ) ,but there were no correlations between the age or histological type. In ovarian cancer tissues, a positive correlation between the expression of HER-2 and TOPO- Ⅱα was observed ( r = 0. 324, P ＜ 0. 05 ) . Conclusion The overexpression of HER-2 and TOPO- Ⅱαplay an important role in ovarian carcinogenesis and development. The expression of HER-2 is positively correlated with TOPO- Ⅱα in ovarian epithelial cancers. Coexpression of the two moleculars may be involved in the development and progression of ovarian epithelial cancer, which should be further studied.