中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2008年
4期
367-371
,共5页
王昌铭%向静%彭昌鼎%王景周%吉国泰
王昌銘%嚮靜%彭昌鼎%王景週%吉國泰
왕창명%향정%팽창정%왕경주%길국태
脐血单个核细胞%血管性痴呆%Pulsinellis四血管阻断法%颈内动脉%脑源性神经营养因子%神经生长因子
臍血單箇覈細胞%血管性癡呆%Pulsinellis四血管阻斷法%頸內動脈%腦源性神經營養因子%神經生長因子
제혈단개핵세포%혈관성치태%Pulsinellis사혈관조단법%경내동맥%뇌원성신경영양인자%신경생장인자
Human cord blood mononuclear cells%Vascular dementia%Pulsinellis 4-vessel occlusion%Intracarotid artery%Brain-derived neurotrophic factor%Nerve growth factor
目的 观察颈内动脉输注脐血单个核细胞(HCMNCs)对血管性痴呆(VD)大鼠脑组织脑源性神经营养因子(BDNF)和神经生长因子(NGF)表达及大鼠学习记忆能力的影响. 方法 改良Pulsinellis四血管阻断法建立VD大鼠模型;大鼠受用随机数字表法分为对照组、模型组和治疗组;每组又分为2,4,8周三个时相点,每时相点12只.体外分离HCMNCs,术后24h颈内动脉输注3x106个BrdU标记细胞于治疗组;利用穿梭箱系统和ELISA法检测注射HCMNCs后2,4,8周VD大鼠学习记忆能力以及脑组织BDNF和NGF含量的变化. 结果 模型组大鼠主动回避反应比率显著低于对照组(P＜0.01),治疗组较模型组显著提高(P＜0.01).术后2周模型组大鼠脑BDNF、NGF含量较对照组显著增高(P＜0.01),4周时达到高峰(P＜0.01),8周时则明显下降,与2,4周时相比有显著性差异(P＜0.05);治疗组大鼠脑BDNF、NGF含量较模型组显著升高(P＜0.01),4周时最高(P＜0.05,P＜0.01),8周时略有下降,但仍维持在较高水平,与2,4周时相比无统计学意义(P＞0.05).结论 颈内动脉输注HCMNCs可显著改善VD大鼠学习记忆能力,推测原因为通过增加VD大鼠脑组织BDNF和NGF发挥作用.
目的 觀察頸內動脈輸註臍血單箇覈細胞(HCMNCs)對血管性癡呆(VD)大鼠腦組織腦源性神經營養因子(BDNF)和神經生長因子(NGF)錶達及大鼠學習記憶能力的影響. 方法 改良Pulsinellis四血管阻斷法建立VD大鼠模型;大鼠受用隨機數字錶法分為對照組、模型組和治療組;每組又分為2,4,8週三箇時相點,每時相點12隻.體外分離HCMNCs,術後24h頸內動脈輸註3x106箇BrdU標記細胞于治療組;利用穿梭箱繫統和ELISA法檢測註射HCMNCs後2,4,8週VD大鼠學習記憶能力以及腦組織BDNF和NGF含量的變化. 結果 模型組大鼠主動迴避反應比率顯著低于對照組(P＜0.01),治療組較模型組顯著提高(P＜0.01).術後2週模型組大鼠腦BDNF、NGF含量較對照組顯著增高(P＜0.01),4週時達到高峰(P＜0.01),8週時則明顯下降,與2,4週時相比有顯著性差異(P＜0.05);治療組大鼠腦BDNF、NGF含量較模型組顯著升高(P＜0.01),4週時最高(P＜0.05,P＜0.01),8週時略有下降,但仍維持在較高水平,與2,4週時相比無統計學意義(P＞0.05).結論 頸內動脈輸註HCMNCs可顯著改善VD大鼠學習記憶能力,推測原因為通過增加VD大鼠腦組織BDNF和NGF髮揮作用.
목적 관찰경내동맥수주제혈단개핵세포(HCMNCs)대혈관성치태(VD)대서뇌조직뇌원성신경영양인자(BDNF)화신경생장인자(NGF)표체급대서학습기억능력적영향. 방법 개량Pulsinellis사혈관조단법건립VD대서모형;대서수용수궤수자표법분위대조조、모형조화치료조;매조우분위2,4,8주삼개시상점,매시상점12지.체외분리HCMNCs,술후24h경내동맥수주3x106개BrdU표기세포우치료조;이용천사상계통화ELISA법검측주사HCMNCs후2,4,8주VD대서학습기억능력이급뇌조직BDNF화NGF함량적변화. 결과 모형조대서주동회피반응비솔현저저우대조조(P＜0.01),치료조교모형조현저제고(P＜0.01).술후2주모형조대서뇌BDNF、NGF함량교대조조현저증고(P＜0.01),4주시체도고봉(P＜0.01),8주시칙명현하강,여2,4주시상비유현저성차이(P＜0.05);치료조대서뇌BDNF、NGF함량교모형조현저승고(P＜0.01),4주시최고(P＜0.05,P＜0.01),8주시략유하강,단잉유지재교고수평,여2,4주시상비무통계학의의(P＞0.05).결론 경내동맥수주HCMNCs가현저개선VD대서학습기억능력,추측원인위통과증가VD대서뇌조직BDNF화NGF발휘작용.
Objective To explore the effects of intracarotid administration of human cord blood mononuclear cells (HCMNCs) on the expressions of brain-derived neurotrophic factor (BDNF)and nerve growth factor (NGF) and the learning-memory abilities in the vascular dementia (VD) rats.Methods The VD rat model was established by modified Pulsinellis 4-vessel occlusion (4 VO). The rats were randomly divided into a control group, a model group and a treatment group. The three groups were observed at three time points: 2 w, 4 w and 8 w, twelve rats in each time point. HCMNCs were isolated from human umbilical cord blood in vitro. The treatment group received intracarotid infusion of 3×106 HCMNCs which were prelabeled with BrdU in vitro. The learning-memory abilities and the contents of BDNF and NGF were observed at 2, 4 and 8 weeks by computerized shuttle-training case and enzyme-linked immunosorbent assay (ELISA). Results Active avoidance response (AAR) ratio in the model group was significantly decreased compared with the control (P＜0.01), and the treatment group was significantly higher than the model group (P＜0.01). The contents of BDNF and NGF in the model group was significantly higher than those in the control, increased to the highest level at 4 weeks, and then decreased at 8 weeks, but compared with that at 2, 4 weeks, significant difference was found (P＜0.05).Com pared with the model group, the contents of BDNF and NGF in the treatment group were significantly increased after infusion with HCMNCs, increased to the highest level at 4 weeks (P＜0.05,P＜0.01), and then decreased a little at 8 weeks but maintained the high level that was not significantly different from that at 2, 4 weeks (P＞0.05). Conclusions Intracarotid administration of HCMNCs significantly improves the learning-memory abilities, possibly through increasing the contents of BDNF and NGF in the VD rats.
