CAJ | 학술논문

目的观察hUHRF1基因对人肿瘤SKOV-3细胞增殖和凋亡的影响.方法小干扰RNA转染肿瘤SKOV-3细胞；实时荧光定量-聚合酶链反应(RT-qPCR)和Western blot法分别检测转染前后hUHRF1 mRNA及蛋白的表达水平；细胞计数试剂盒(CCK-8)试验检测细胞增殖；流式细胞术分析细胞凋亡.结果 hUHRF1 mRNA在转染组表达显著降低(P＜0.01)；转染组、阴性组和空白组蛋白相对表达量分别为0.72±0.42、1.66±0.27和1.62±0.29.干扰后,细胞增殖减少(P＜0.05)；各组别细胞凋亡率分别为(42.320±4.174)％、(17.740±1.786)％和(15.440±1.233)％结论小干扰RNA沉默技术可以显著抑制肿瘤SKOV-3细胞hUHRF1的表达,有效抑制细胞增殖,并显著诱导细胞凋亡.
목적 관찰hUHRF1기인대인종류SKOV-3세포증식화조망적영향.방법 소간우RNA전염종류SKOV-3세포；실시형광정량-취합매련반응(RT-qPCR)화Western blot법분별검측전염전후hUHRF1 mRNA급단백적표체수평；세포계수시제합(CCK-8)시험검측세포증식；류식세포술분석세포조망.결과 hUHRF1 mRNA재전염조표체현저강저(P＜0.01)；전염조、음성조화공백조단백상대표체량분별위0.72±0.42、1.66±0.27화1.62±0.29.간우후,세포증식감소(P＜0.05)；각조별세포조망솔분별위(42.320±4.174)％、(17.740±1.786)％화(15.440±1.233)％결론소간우RNA침묵기술가이현저억제종류SKOV-3세포hUHRF1적표체,유효억제세포증식,병현저유도세포조망.
Objective To discuss the effects of hUHRF1 gene on proliferation and apoptosis effects of human ovarian cancer cell line SKOV-3.Methods Small interfering RNA (siRNA) for hUHRF1 gene sequence was transfected into SKOV-3 cells,Real-time quantitative polymerase chain reaction ( RTqPCR) and Western blotting assays were used to detect the mRNA and protein expression levels of hUHRF1 respectively before and after siRNA transfection.Cell counting Kit-8 (CCK-8) assay was used to investigate proliferation.Flow cytometry was used to measure apoptosis.Results mRNA expression of hUHRF1 was significantly decreased in SKOV-3 cells after the transfection of siRNA ( P ＜ 0.01 ).Relative expression of hUHRF1 protein in transfection group,negative control group and blank group was 0.72 ± 0.42,1.66 ± 0.27 and 1.62 ± 0.29 respectively.The proliferation of SKOV-3 cells was inhibited after transfection.Apoptosis rate of SKOV-3 cells in transfectiou group,negative control group and blank group was (42.320 ±4.174)％,( 17.740 ± 1.786)％ and ( 15.440 ± 1.233)％,respectively.Conclusion Using the siRNA gene silencing techniques,the expression of hUHRF1 is significantly inhibited after gene silencing in SKOV-3 cells, which can effectively inhibited SKOV-3 cell proliferation and iuduced apoptosis.