针灸推拿医学(英文版)
針灸推拿醫學(英文版)
침구추나의학(영문판)
JOURNAL OF ACUPUNCTURE AND TUINA SCIENCE
2008年
1期
4-7
,共4页
刘慧荣%谭琳蓥%吴焕淦%朱毅%赵粹英%崔云华%江彬%王晓梅
劉慧榮%譚琳鎣%吳煥淦%硃毅%趙粹英%崔雲華%江彬%王曉梅
류혜영%담림형%오환감%주의%조수영%최운화%강빈%왕효매
针灸疗法%结肠炎%溃疡性%成纤维细胞%胶原%大鼠
針灸療法%結腸炎%潰瘍性%成纖維細胞%膠原%大鼠
침구요법%결장염%궤양성%성섬유세포%효원%대서
Acupuncture-Moxibustion Therapy%Colitis,Ulcerative%Fibroblasts%Collagen%Rats
目的:从对溃疡性结肠炎大鼠结肠成纤维细胞(Colonic Fibroblast,CFB)合成分泌胶原的影响的角度,探讨艾灸防治溃疡性结肠炎肠纤维化的作用机制.方法:采用免疫法加局部刺激制备溃疡性结肠炎肠纤维化大鼠模型,随机将大鼠分为正常组、模型组、隔药灸组、温和灸组和西药组.隔药灸组、温和灸组选取天枢、气海穴分别进行隔药灸、温和灸治疗,西药组柳氮磺胺吡啶溶液灌胃治疗.分离并培养结肠成纤维细胞,ELISA法观察艾灸对溃疡性结肠炎肠纤维化大鼠结肠成纤维细胞合成分泌Ⅰ、Ⅲ、Ⅳ型胶原的影响.结果:UC纤维化大鼠CFB培养上清液能显著激发正常大鼠CFB大量分泌Ⅰ、Ⅲ、Ⅳ型胶原,隔药灸组、温和灸组上清液则显著抑制正常大鼠CFB Ⅰ、Ⅲ、Ⅳ型胶原的分泌,西药组上清液对Ⅰ、Ⅲ型胶原分泌有一定抑制作用.结论:艾灸能够调节溃疡性结肠炎肠纤维化大鼠结肠成纤维细胞合成分泌Ⅰ、Ⅲ、Ⅳ型胶原.
目的:從對潰瘍性結腸炎大鼠結腸成纖維細胞(Colonic Fibroblast,CFB)閤成分泌膠原的影響的角度,探討艾灸防治潰瘍性結腸炎腸纖維化的作用機製.方法:採用免疫法加跼部刺激製備潰瘍性結腸炎腸纖維化大鼠模型,隨機將大鼠分為正常組、模型組、隔藥灸組、溫和灸組和西藥組.隔藥灸組、溫和灸組選取天樞、氣海穴分彆進行隔藥灸、溫和灸治療,西藥組柳氮磺胺吡啶溶液灌胃治療.分離併培養結腸成纖維細胞,ELISA法觀察艾灸對潰瘍性結腸炎腸纖維化大鼠結腸成纖維細胞閤成分泌Ⅰ、Ⅲ、Ⅳ型膠原的影響.結果:UC纖維化大鼠CFB培養上清液能顯著激髮正常大鼠CFB大量分泌Ⅰ、Ⅲ、Ⅳ型膠原,隔藥灸組、溫和灸組上清液則顯著抑製正常大鼠CFB Ⅰ、Ⅲ、Ⅳ型膠原的分泌,西藥組上清液對Ⅰ、Ⅲ型膠原分泌有一定抑製作用.結論:艾灸能夠調節潰瘍性結腸炎腸纖維化大鼠結腸成纖維細胞閤成分泌Ⅰ、Ⅲ、Ⅳ型膠原.
목적:종대궤양성결장염대서결장성섬유세포(Colonic Fibroblast,CFB)합성분비효원적영향적각도,탐토애구방치궤양성결장염장섬유화적작용궤제.방법:채용면역법가국부자격제비궤양성결장염장섬유화대서모형,수궤장대서분위정상조、모형조、격약구조、온화구조화서약조.격약구조、온화구조선취천추、기해혈분별진행격약구、온화구치료,서약조류담광알필정용액관위치료.분리병배양결장성섬유세포,ELISA법관찰애구대궤양성결장염장섬유화대서결장성섬유세포합성분비Ⅰ、Ⅲ、Ⅳ형효원적영향.결과:UC섬유화대서CFB배양상청액능현저격발정상대서CFB대량분비Ⅰ、Ⅲ、Ⅳ형효원,격약구조、온화구조상청액칙현저억제정상대서CFB Ⅰ、Ⅲ、Ⅳ형효원적분비,서약조상청액대Ⅰ、Ⅲ형효원분비유일정억제작용.결론:애구능구조절궤양성결장염장섬유화대서결장성섬유세포합성분비Ⅰ、Ⅲ、Ⅳ형효원.
Objective: To investigate the mechanisms of moxibustion in the treatment of the colonic fibrosis in ulcerative colitis (UC) by observing the colonic fibroblast (CFB) synthesizing and secreting collagen in ulcerative colitis fibrosis rats. Methods: A rat model of ulcerative colitis fibrosis was established by immunological methods using human colonic mucosa as antigen adding local stimulation. The rats were randomly divided into normal group, model group, herb-partition moxibustion group, mild-warm moxibustion group and western medicine group (SASP group). Herb-partitioned moxibustion group and mild-warm moxibustion group treated by herb-partitioned moxibustion and mild-warm moxibustion respectively on Qihai (CV 6) and Tianshu (ST 25, bilateral) points. SASP group fed with salicylazosulfapyridine. Colonic fibroblasts from all the rats were isolated and cultured and the effects of moxibustion on the colonic fibroblast synthesizing and secreting type I, III, and IV collagen were observed. Results: The supernatant of cultured CFB from UC rats could stimulate the CFB of normal rats to secrete type I, III, and IV collagens. The supernatant from rats treated by herb-partitioned moxibustion and mild-warm moxibustion inhibited the secretion of type I , III, and IV collagens of CFB in normal rats. And the western medicine group also had some inhibiting effects on the type I and HI collagens. Conclusion: Moxibustion can regulate the functions of CFB synthesizing and secreting type I, III, and IV collagens in ulcerative colitis fibrosis rats.