CAJ | 학술논문

研究了不同Cu~(2+)处理浓度(0、 0.002、 0.004、 0.006、 0.008 mmol·L~(-1))对菱叶片叶绿素含量和叶绿素荧光参数、膜脂过氧化和抗氧化系统等的影响,并用透射电镜观察了其叶细胞超微结构的变化.试验结果表明:随着Cu~(2+)浓度的增加:(1)叶绿素含量、Fv/Fo和Fv/Fm显著下降,Fo显著上升;(2)活性氧(AOS)产生速率、丙二醛(MDA)含量、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性逐渐升高;超氧化物歧化酶(SOD)、游离脯氨酸(Pro)和可溶性糖含量显著下降;抗坏血酸(AsA)、还原型谷胱甘肽(GSH)和谷胱甘肽还原酶(GR)含量呈先升后降趋势,且始终高于对照;(3)电镜观察发现,Cu~(2+)对叶片细胞器的超微结构特别是叶绿体、线粒体和细胞核造成严重损伤.以上结果表明,本试验过量Cu~(2+)的胁迫破坏了菱正常生理生化活动,并造成功能紊乱,最终导致菱的死亡.
연구료불동Cu~(2+)처리농도(0、 0.002、 0.004、 0.006、 0.008 mmol·L~(-1))대릉협편협록소함량화협록소형광삼수、막지과양화화항양화계통등적영향,병용투사전경관찰료기협세포초미결구적변화.시험결과표명:수착Cu~(2+)농도적증가:(1)협록소함량、Fv/Fo화Fv/Fm현저하강,Fo현저상승;(2)활성양(AOS)산생속솔、병이철(MDA)함량、과양화물매(POD)、과양화경매(CAT)화항배혈산과양화물매(APX)활성축점승고;초양화물기화매(SOD)、유리포안산(Pro)화가용성당함량현저하강;항배혈산(AsA)、환원형곡광감태(GSH)화곡광감태환원매(GR)함량정선승후강추세,차시종고우대조;(3)전경관찰발현,Cu~(2+)대협편세포기적초미결구특별시협록체、선립체화세포핵조성엄중손상.이상결과표명,본시험과량Cu~(2+)적협박파배료릉정상생리생화활동,병조성공능문란,최종도치릉적사망.
The toxic effects of various concentrations of Cu~(2+) (0, 0.002, 0.004, 0.006, 0.008 mmol·L~(-1)) were studied on chlorophyll content,chlorophyll fluorescence parameters,lipid peroxidation,antioxidant system and ultrastructure changes in leafs of Trapa bispinosa Roxb.The results showed that with the rise of Cu~(2+) concentration in culture medium,(1)the chlorophyll contents and chlorophyll fluorescence parameters (Fv/Fo,Fv/Fm) decreased significantly,while Fo increased gradually.(2)generating rate of active oxygen(AOS),malondialdehyde(MDA) content and the activities of peroxidase (POD),catalase(CAT) as well as ascorbate peroxidase (APX) all increased gradually.Superoxide dismutase(SOD) activity,free proline(Pro) and soluble sugar contents increased markedly.While the contents of ascorbic acid(ASA),glutathione(GSH) and glutathione reductase (GR) activity enhanced progressively and followed by a decline at the higher doses.(3)Transmission electron microscopy observation indicates that the higher Cu~(2+) concentration,the more serious damages to the leaf cells,especially chloroplast,mitochondria and nucleus.Thus,the detriment effect of Cu~(2+) of Trapa bispinosa Roxb.was resulted from disorder of physiological,biochemical metabolism and disorder of physiological metabolism.