CAJ | 학술논문

采用随机扩增多态DNA(RAPD)技术对健康和自咬水貂群体进行检测,并在此基础上进行了RAPD向序列特异性扩增(SCAR)标记的转化.从100个RAPD随机引物中筛选出5个重复性好的引物.通过对引物(A1)的扩增能够在两群体中找到差异标记(HA-400)和共有标记(SA-500),对其进行克隆、测序,并根据测序结果设计两对SCAR引物(SHA-400 和SSA-500).SHA-400和SSA-500在健康和自咬群体中均有扩增.其中,SHA-400在两个群体扩增频率分别为82.5%和22.5%,差异极显著(P<0.001);SSA-500在两群体的扩增频率为87.5% 和97.5%,差异不显著(P>0.05).结果表明:HA-400可初步作为区分健康和患病水貂群体的分子遗传标记.
채용수궤확증다태DNA(RAPD)기술대건강화자교수초군체진행검측,병재차기출상진행료RAPD향서렬특이성확증(SCAR)표기적전화.종100개RAPD수궤인물중사선출5개중복성호적인물.통과대인물(A1)적확증능구재량군체중조도차이표기(HA-400)화공유표기(SA-500),대기진행극륭、측서,병근거측서결과설계량대SCAR인물(SHA-400 화SSA-500).SHA-400화SSA-500재건강화자교군체중균유확증.기중,SHA-400재량개군체확증빈솔분별위82.5%화22.5%,차이겁현저(P<0.001);SSA-500재량군체적확증빈솔위87.5% 화97.5%,차이불현저(P>0.05).결과표명:HA-400가초보작위구분건강화환병수초군체적분자유전표기.
RAPD was used to analyze genetic constitution of the healthy and self-biting mink groups, and was converted into SCAR marker. Five polymorphic primers were screened from 100 random primers by RAPD. Specific and non-specific amplified DNA fragments, HA-400 and SA-500, were found, cloned and sequenced. Based on these sequences, two specific primers of SCAR were designed (SHA-400 and SSA-500). Then PCR amplification was carried out for the healthy and self-biting minks. Results showed that almost all of mink were amplified by SHA-400 and SSA-500. The frequency of SHA-400 in the two groups was 82.5% and 22.5% respectively, and χ~2 test of polymorphic fragments in the two groups showed significantly difference (P<0.001). The frequency of SSA-500 in the two groups was 87.5% and 97.5% respectively, and no significant difference was observed between the two groups (P>0.05). It is indicated that the polymorphic fragment (HA-400) can be used as a positive marker candidate.