CAJ | 학술논문

萜类化合物是重要的昆虫拒食剂类型.研究采用叶碟浸液法测试松节油、羟基香茅醛、薄荷醇及其衍生合成的多个系列的萜类化合物对蔬菜害虫萝卜蚜(Lipaphis erysimi)的拒食活性.结果显示,当质量浓度为10mg/mL时,24h后有22种化合物的拒食率高于75%,其中诺卜醇、内型异莰烷基甲醇乙酸酯、4-(1-甲基乙烯基)-1-环己烯-1-乙醇丙酸酯、诺卜甲基醚、8-羟基别二氢葛缕醇、薄荷醇、8-羟基别二氢葛缕醇甲酸酯、8-羟基别二氢葛缕醇乙酸酯、8-羟基别二氢葛缕醇丙酸酯、羟基香茅醛乙基醚、蒎酮酸甲酯等11种化合物的拒食率达到100%.当浓度为1mg/mL时,这些化合物的拒食活性出现了较大的差别,其中2个化合物即内型异莰烷基甲醇丙酸酯和内型1-异莰烷基-3-己醇乙酸酯的拒食活性最高,且持效性较好.系列等比浓度测试结果显示,内型1-异莰烷基-3-己醇乙酸酯的剂量-活性回归方程的坡度值在12,24,36,48,72h为0.47～1.72,拒食中浓度(AFC_50)为4.0～12.1mg/mL.8号化合物(内型异莰烷基甲醇丙酸酯)的坡度值为1.08～2.91, AFC_(50)为2.6～7.0mg/mL,表现出良好的正向剂量活性效应和持效性,是一种具有应用前景的蚜虫拒食剂.
첩류화합물시중요적곤충거식제류형.연구채용협설침액법측시송절유、간기향모철、박하순급기연생합성적다개계렬적첩류화합물대소채해충라복아(Lipaphis erysimi)적거식활성.결과현시,당질량농도위10mg/mL시,24h후유22충화합물적거식솔고우75%,기중낙복순、내형이감완기갑순을산지、4-(1-갑기을희기)-1-배기희-1-을순병산지、낙복갑기미、8-간기별이경갈루순、박하순、8-간기별이경갈루순갑산지、8-간기별이경갈루순을산지、8-간기별이경갈루순병산지、간기향모철을기미、파동산갑지등11충화합물적거식솔체도100%.당농도위1mg/mL시,저사화합물적거식활성출현료교대적차별,기중2개화합물즉내형이감완기갑순병산지화내형1-이감완기-3-기순을산지적거식활성최고,차지효성교호.계렬등비농도측시결과현시,내형1-이감완기-3-기순을산지적제량-활성회귀방정적파도치재12,24,36,48,72h위0.47～1.72,거식중농도(AFC_50)위4.0～12.1mg/mL.8호화합물(내형이감완기갑순병산지)적파도치위1.08～2.91, AFC_(50)위2.6～7.0mg/mL,표현출량호적정향제량활성효응화지효성,시일충구유응용전경적아충거식제.
Terpenoid compounds are important chemicals in insect antifeedants. In this study,the antefeedant activities of turpentine oil,hydroxycitronellal,menthol and their derivatives synthesized in a project against turnip aphid,Lipaphis erysimi (Kaltenbach),were tested using the method of dipped leaf discs.22 compounds showed antifeedant activities more than 75% 24 h after the treatment at the concentration of 10 mg/mL,and eleven of which,i. e. nopol,endo-isocamphanyl-methyl acetate,4-(1-methylethylene)-1-cyclohexenyl -1-ethol propionate,nopyl methyl ether,8-hydroxylcarveol,menthol,8-hydroxylcarveol formate,8-hydroxylcarveol acetate,8-hydroxylcarveol propionate,hydroxycitronellal ethyl ether,methyl pinonate,held the antifeedant rates as much as 100%.15 compounds were further tested with the concentration of 1 mg/mL,and their activities were differentiated much with the antefeedant rates from -36% to 79% 24 h later.Compounds 8 and 10,namely endo-isocamphanyl-methyl propionate and endo-1-isocamphanyl-3-hexyl acetate,comprehensively held the best action and residual efficacy.The results of probit analysis showed that the slopes of dosage-action linear regression of compound 8 varied from 1.08 to 2.91; AFC_(50)s were from 2.6 mg/mL to 7.0 mg/mL at the observation time points of 12 h,24 h,36 h,48 h and 72 h after treatment.The slopes of compound 10 varied from 0.47 to 1.72; AFC_(50) s were from 4.6 mg/mL to 12.2 mg/mL. It is suggested that endo-isocamphanyl-methyl propionate would be a promising antifeedant in aphis control.