台湾海峡
檯灣海峽
태만해협
JOURNAL OF OCEANOGRAPHY IN TAIWAN STRAIT
2010年
1期
128-134
,共7页
海洋生物学%尼罗红%海洋%油脂真菌%脂肪酸%方法
海洋生物學%尼囉紅%海洋%油脂真菌%脂肪痠%方法
해양생물학%니라홍%해양%유지진균%지방산%방법
marine biology%Nile red%ocean oleaginous%microorganisms%fatty acid%method
选取分离自大洋、深海的28株酵母和143株霉菌,采用在培养基中添加尼罗红比较表观荧光强度的方法来筛选出酵母2株和霉菌7株,但菌体呈红色的酵母(17株)及菌丝为黑色的霉菌(28株)不适用于此法;通过单位OD600荧光强度的高低筛选出1株菌落为微红色的酵母,产油性能好于用第一种方法挑选出的2株酵母,且菌体颜色对结果并无影响,但只适合筛选酵母.通过上述2种方法筛选出高油脂菌株,大多来源于深海.
選取分離自大洋、深海的28株酵母和143株黴菌,採用在培養基中添加尼囉紅比較錶觀熒光彊度的方法來篩選齣酵母2株和黴菌7株,但菌體呈紅色的酵母(17株)及菌絲為黑色的黴菌(28株)不適用于此法;通過單位OD600熒光彊度的高低篩選齣1株菌落為微紅色的酵母,產油性能好于用第一種方法挑選齣的2株酵母,且菌體顏色對結果併無影響,但隻適閤篩選酵母.通過上述2種方法篩選齣高油脂菌株,大多來源于深海.
선취분리자대양、심해적28주효모화143주매균,채용재배양기중첨가니라홍비교표관형광강도적방법래사선출효모2주화매균7주,단균체정홍색적효모(17주)급균사위흑색적매균(28주)불괄용우차법;통과단위OD600형광강도적고저사선출1주균락위미홍색적효모,산유성능호우용제일충방법도선출적2주효모,차균체안색대결과병무영향,단지괄합사선효모.통과상술2충방법사선출고유지균주,대다래원우심해.
Two methods for rapid screening intracellular lipid content in oceanic oleaginous microorganisms (28 yeasts and 143 moulds) were established using a fluorescent probe Nile red in this paper. The first method screened oceanic oleaginous microorganisms using Nile red, established by the Nile red directly included in the medium and then compared the fluorescence intensity under ultraviolet. 2 white yeasts out of 28 yeasts and 7 non-black moulds out of 143 moulds were prominent in producing intracellular fatty acid. However, this method was not suitable for red yeasts(17) and black moulds(28). The second method, by comparing the fluorescence intensity per OD600, one reddish yeast was selected with much higher fluorescence intensity. However, this method could not apply to moulds due to their inter-twisted mycelia. With above methods most of screened microorganisms capable of producing high content intracellular fatty acid are from deep sea.
