CAJ | 학술논문

目的探讨核基质附着区结合蛋白(MARBP)与皮肤鳞状细胞癌(SCC)的相关性,筛选相关性较高的MARBP.方法以取材自2例SCC患者正常皮肤组织和HaCaT细胞作为对照,应用RT-PCR方法,检测部分已知与肿瘤相关的MARBP(CUX1、Lamin B1、Nucleolin、hnRNP U、SAFB2、SATB1、YBX1)mRNA在12例SCC肿瘤组织和瘤旁组织、人皮肤鳞状细胞癌细胞系Colo-16细胞、人表皮癌A431细胞的表达水平.结果所有被检测的MARBP mRNA在2例正常皮肤组织均未见明显表达,在12例SCC肿瘤组织和瘤旁组织、Colo-16、A431细胞系中均有不同程度和不同比例的表达,在HaCaT细胞系中,除SATB1外,其余MARBP mRNA均有表达.SCC肿瘤组织和瘤旁组织MARBP mRNA表达水平的比较显示CUX1、Nucleolin和YBX1差异有统计学意义;Lamin B1、hnRNP U、SAFB2和SATB1差异无统计学意义.结论 CUX1、Nucleolin和YBX1等MARBP mRNA可能参与SCC的发生、发展.
목적 탐토핵기질부착구결합단백(MARBP)여피부린상세포암(SCC)적상관성,사선상관성교고적MARBP.방법 이취재자2례SCC환자정상피부조직화HaCaT세포작위대조,응용RT-PCR방법,검측부분이지여종류상관적MARBP(CUX1、Lamin B1、Nucleolin、hnRNP U、SAFB2、SATB1、YBX1)mRNA재12례SCC종류조직화류방조직、인피부린상세포암세포계Colo-16세포、인표피암A431세포적표체수평.결과 소유피검측적MARBP mRNA재2례정상피부조직균미견명현표체,재12례SCC종류조직화류방조직、Colo-16、A431세포계중균유불동정도화불동비례적표체,재HaCaT세포계중,제SATB1외,기여MARBP mRNA균유표체.SCC종류조직화류방조직MARBP mRNA표체수평적비교현시CUX1、Nucleolin화YBX1차이유통계학의의;Lamin B1、hnRNP U、SAFB2화SATB1차이무통계학의의.결론 CUX1、Nucleolin화YBX1등MARBP mRNA가능삼여SCC적발생、발전.
Objective To estimate the relationship between matrix attachment region-binding proteins (MARBPs) and skin squamous cell carcinoma (SCC), and to screen for highly-associated MARBPs. Methods Skin specimens were resected from the tumor and tumor-adjacent normal tissue of 12 patients with SCC. Normal skin tissue from the abdomen of 2 of the 12 patients served as the control. Reverse-transcription PCR was performed to detect the mRNA expressions of seven MARBPs, including CUX1, Lamin Bl, Nucleolin, hnRNP U,SAFB2, SATB1 and YBX1, in these specimens and in a human keratinocyte cell line HaCaT and two cell lines Colo-16 and A431 derived from human SCC. Results No evident expression was observed for the seven MARBPs in the normal control specimens, while the MARBPs were expressed in the tumor tissue and tumoradjacent tissue specimens, Colo-16 cells and A431 cells at different degrees and frequencies. In HaCaT cells,all the 7 MARBPs except SATB1 were expressed. Statistical analysis showed a significant difference in the expression of CUX1, Nucleolin and YBX1, but not in that of Lamin Bl, hnRNP U, SAFB2 or SATB1 between the tumor and tumor-adjacent normal tissues. Conclusion Some MARBPs such as CUX1, Nucleolin and YBX1 may participate in the development of skin squamous cell carcinoma.