CAJ | 학술논문

目的观察裸鼠经犬钩虫第三期钩蚴(AcL3)免疫后,其皮肤和肺内AcL3的形态变化及宿主的组织细胞反应,评价其作为疫苗筛选动物模型的可能性. 方法取BALB/c-nu/nu 小鼠,每两周由皮下免疫接种活的AcL3 500条,共3次,并丁末次免疫后1周由皮肤攻击感染AcL3 500条.用未免疫的感染AcL3 裸鼠作对照,攻击感染后不同时间取感染部位皮肤和肺脏,观察宿卡皮肤和肺内AcL3的组织病理学变化. 结果攻击感染后6、24、72 h及7 d,皮肤内的绝人部分虫体切面形态和组织结构与感染对照裸鼠皮肤内的相似,仪0.5%～2.2%的虫体切而示有变性、死亡,偶见有死虫肉芽肿,而肺组织内的AcL3及其周围的炎细胞反应对照组相比较,无明显差异. 结论用活的AcL3免疫裸鼠后,仅见对攻击感染的AcL3 有弱的免疫效应,裸鼠不适宜作为钩虫疫苗筛选的动物模型.
목적 관찰라서경견구충제삼기구유(AcL3)면역후,기피부화폐내AcL3적형태변화급숙주적조직세포반응,평개기작위역묘사선동물모형적가능성. 방법취BALB/c-nu/nu 소서,매량주유피하면역접충활적AcL3 500조,공3차,병정말차면역후1주유피부공격감염AcL3 500조.용미면역적감염AcL3 라서작대조,공격감염후불동시간취감염부위피부화폐장,관찰숙잡피부화폐내AcL3적조직병이학변화. 결과공격감염후6、24、72 h급7 d,피부내적절인부분충체절면형태화조직결구여감염대조라서피부내적상사,의0.5%～2.2%적충체절이시유변성、사망,우견유사충육아종,이폐조직내적AcL3급기주위적염세포반응대조조상비교,무명현차이. 결론용활적AcL3면역라서후,부견대공격감염적AcL3 유약적면역효응,라서불괄의작위구충역묘사선적동물모형.
Objective To observe the inflammatory responses and morphology changes in the lung and skin of nude mice immunized and challenged with third stage hookworm larvae of Ancylostoma caninum (AcL3),and then,to investigate the feasibility of nude mice as vaccine screening animal model.Methods Nude mice BALB/c-nu/nu were immunized subcutaneously with three doses of 500 AcL3 at 2-week intervals,and then challenged percutaneously with 500 AcL3.Lungs and skins were excised from post-challenged nude mice after 6,24,72 h and 7 d,and then examined by light microscopy.Non-immunized nude mice served as negative controls.Results In both non-immunized mice and majority of immunized mice,the AcL3 exhibited no structural damage and infiltrating inflammatory cells were absent at the surrounding tissues.There were no changes in the architecture of skin and lung tissues.However.about 0.5%-2.2% AcL3 in the skin of immunized mice exhibited euticular swelling,damage and even death,and the surrounding tissue was infihrated by polymorphonuclear inflammatory cells.From 24 h to 72 h post-challenge,granulomata were observed surrounding the dead AcL3.ConchLsion Weak post-vaccination host immune response against challenged AcL3 was seen in nude mice,indicating that it was unsuitable to be used as vaccine screening animal model.