中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2010年
5期
540-543
,共4页
孟凡旭%何淑梅%董娟聪%郭海卓%金顺子
孟凡旭%何淑梅%董娟聰%郭海卓%金順子
맹범욱%하숙매%동연총%곽해탁%금순자
电离辐射%调节性T细胞%叉头状转录因子%免疫效应
電離輻射%調節性T細胞%扠頭狀轉錄因子%免疫效應
전리복사%조절성T세포%차두상전록인자%면역효응
Ionizing radiation%Regulatory T-Lymphocytes%Forkhead box protein-3 (Foxp3)%Immune response
目的 观察不同剂量X射线照射后不同时间小鼠脾细胞中调节性T细胞和叉头状转录因子(Foxp3)的表达变化,探讨X射线对调节性T细胞以及相关因子Foxp3的影响.方法 112只雄性ICR小鼠,随机平均分为低剂量(0.075 Gy)组和高剂量(2 Gy)组,用X射线深部治疗机分别进行0.075和2 Gy全身照射,于照射后0、4、8、16、24、48和72 h处死,取脾脏,分别应用流式细胞术和RT-PCR法检测调节性T细胞和Foxp3的表达水平.结果 0.075和2 Gy X射线全身照射后,小鼠脾细胞CD4+CD25+Treg细胞百分比均在照射后8 h达高峰,随后一直保持较高水平,高于照射前水平(2 Gy,在8、16、48、72 h时,t=4.65、4.28、3.71、2.88,P＜0.05;0.075 Gy,在8、16、24、72 h时,t=8.73、10.55、4.21、4.65,P＜0.05).Foxp3在mRNA水平,0.075 Gy照射后变化不明显,而2 Gy照射后于24 h形成峰值.Foxp3在蛋白质水平,0.075 Gy照射后并未有显著变化;而2 Gy照射后于16 h形成峰值,随后略有下降,但仍保持较高水平.结论 调节性T细胞及相关分子Foxp3的不同变化可能成为高、低剂量X射线诱导不同免疫效应的原因之一.
目的 觀察不同劑量X射線照射後不同時間小鼠脾細胞中調節性T細胞和扠頭狀轉錄因子(Foxp3)的錶達變化,探討X射線對調節性T細胞以及相關因子Foxp3的影響.方法 112隻雄性ICR小鼠,隨機平均分為低劑量(0.075 Gy)組和高劑量(2 Gy)組,用X射線深部治療機分彆進行0.075和2 Gy全身照射,于照射後0、4、8、16、24、48和72 h處死,取脾髒,分彆應用流式細胞術和RT-PCR法檢測調節性T細胞和Foxp3的錶達水平.結果 0.075和2 Gy X射線全身照射後,小鼠脾細胞CD4+CD25+Treg細胞百分比均在照射後8 h達高峰,隨後一直保持較高水平,高于照射前水平(2 Gy,在8、16、48、72 h時,t=4.65、4.28、3.71、2.88,P＜0.05;0.075 Gy,在8、16、24、72 h時,t=8.73、10.55、4.21、4.65,P＜0.05).Foxp3在mRNA水平,0.075 Gy照射後變化不明顯,而2 Gy照射後于24 h形成峰值.Foxp3在蛋白質水平,0.075 Gy照射後併未有顯著變化;而2 Gy照射後于16 h形成峰值,隨後略有下降,但仍保持較高水平.結論 調節性T細胞及相關分子Foxp3的不同變化可能成為高、低劑量X射線誘導不同免疫效應的原因之一.
목적 관찰불동제량X사선조사후불동시간소서비세포중조절성T세포화차두상전록인자(Foxp3)적표체변화,탐토X사선대조절성T세포이급상관인자Foxp3적영향.방법 112지웅성ICR소서,수궤평균분위저제량(0.075 Gy)조화고제량(2 Gy)조,용X사선심부치료궤분별진행0.075화2 Gy전신조사,우조사후0、4、8、16、24、48화72 h처사,취비장,분별응용류식세포술화RT-PCR법검측조절성T세포화Foxp3적표체수평.결과 0.075화2 Gy X사선전신조사후,소서비세포CD4+CD25+Treg세포백분비균재조사후8 h체고봉,수후일직보지교고수평,고우조사전수평(2 Gy,재8、16、48、72 h시,t=4.65、4.28、3.71、2.88,P＜0.05;0.075 Gy,재8、16、24、72 h시,t=8.73、10.55、4.21、4.65,P＜0.05).Foxp3재mRNA수평,0.075 Gy조사후변화불명현,이2 Gy조사후우24 h형성봉치.Foxp3재단백질수평,0.075 Gy조사후병미유현저변화;이2 Gy조사후우16 h형성봉치,수후략유하강,단잉보지교고수평.결론 조절성T세포급상관분자Foxp3적불동변화가능성위고、저제량X사선유도불동면역효응적원인지일.
Objective To observe the changes in expressions of spleen regulatory T cells (Tregs)and the related factor forkhead box protein-3 (Foxp3) after irradiation with different doses of X-ray in mice at different times,and to elaborate the effects of X-rays on regulatory T cells and Foxp3.Methods 112male ICR mice were randomly divided into 2 groups and irradiated by X-rays at the doses of 0.075 and 2 Gy,respectively.The mice were killed at 0,4,8,16,24,48,and 72 h post-irradiation and the spleens removed.Flow cytometry was used to detect the percentage of CD4 + CD25 + Treg and protein expression of (Foxp3),and RT-PCR was used to exmiamine the mRNA expression of Fox3.Results Compared with those before irradiation,the CD4 + CD25 + Treg positive rates began to increase and peaked at 8 h post-irradiation with 0.075 Gy at 8,16,24,72 h(t = 8.73,10.55,4.21,4.65 ,P ＜ 0.05) and 2 Gy at 8,16,48,72 h(t = 4.65,4.28,3.71,2.88,P ＜ 0.05),and then slightly decreased,but still remained at high levels.The mRNA protein levels of Fox3 did not change significantly after exposure to the dose of 0.075 Gy,but began to significantly increase at 8 h after exposure to the dose of 2 Gy.However,the Foxp3 protein level began to increase 4 h post-irradiation,peaked at 16 h,and then slightly decreased,but still ramained at high levels (t =2.59,3.37,3.70,3.20,P＜0.05).Conclusions The changes in expressions of Tregs and Foxp3 after high- and low-dose X-ray irradiation may be used to explain the differences in immune effects induced by ionizing radiation at different doses.
