CAJ | 학술논문

目的探讨Sonic hedgehog(SHH)在肝细胞癌(HCC)和癌周肝组织中的表达及其临床意义.方法检测145例HCC和癌周肝组织SHH的表达,了解其表达与患者根治性切除术后预后的关系.检测4种肝癌细胞株以及正常肝细胞株SHH mRNA的含量.结果 SHH主要表达于肝细胞和肝癌细胞的胞质,肝癌与癌周肝组织表达差异无统计学意义(P＞0.05).癌和癌周高表达的患者总体生存率显著高于低表达患者(P＜0.05).肝癌细胞株SHH表达量显著高于正常肝细胞株(P＜0.01).结论 SHH在HCC和癌周肝组织中高表达,其表达可用于区分患者预后,SHH可能参与HCC的发生和发展.
목적 탐토Sonic hedgehog(SHH)재간세포암(HCC)화암주간조직중적표체급기림상의의.방법 검측145례HCC화암주간조직SHH적표체,료해기표체여환자근치성절제술후예후적관계.검측4충간암세포주이급정상간세포주SHH mRNA적함량.결과 SHH주요표체우간세포화간암세포적포질,간암여암주간조직표체차이무통계학의의(P＞0.05).암화암주고표체적환자총체생존솔현저고우저표체환자(P＜0.05).간암세포주SHH표체량현저고우정상간세포주(P＜0.01).결론 SHH재HCC화암주간조직중고표체,기표체가용우구분환자예후,SHH가능삼여HCC적발생화발전.
Objective To investigate the expression of Sonic hedgehog (SHH) in paired tumor and peritumoral liver tissues of hepatocellular carcinoma (HCC) and its clinical significance.Methods The expression of SHH was assessed by immunohistochemistry in tissue microarrays containing paired tumor and peritumoral liver tissues from 145 patients who received hepatectomy for histologically proven HCC.Prognostic value of SHH expression was evaluated.The SHH mRNA expression of four HCC cell lines (HCCLM3,MHCC97H,MHCC97L,HepG2) and a normal liver cell line (L-02) was detected by realtime reverse transcription-polymerase chain reaction (RT-PCR).Results SHH was mainly expressed in the cytoplasm of tumor cells or hepatocytes in peritumoral or normal liver tissues.There was no statistically significant difference in the SHH density betwen intratumoral and peritumoral liver tissues ( P ＞ 0.05 ).The median overall survival (OS) of HCC patients with high intratumoral SHH expression was longer than low SHH expression patients (P ＜0.05).High peritumoral SHH expression was also associated with good survival (P ＜0.05).SHH was expressed positively in both HCC and normal cell lines,and the levels of mRNA was higher in HCC cell lines than in L-02 ( P ＜ 0.01 ).Conclusion SHH is expressed higher in both intratumoral or peritumoral liver tissues,and the expression of SHH is significantly correlated with OS of patients with HCC.SHH may become a biomarker of HCC patients after curative resection.