中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2011年
6期
474-476
,共3页
叶军%陈亚宝%徐洪涛%成建春%张立新%林梅%黄俊星
葉軍%陳亞寶%徐洪濤%成建春%張立新%林梅%黃俊星
협군%진아보%서홍도%성건춘%장립신%림매%황준성
肝炎病毒,乙型%T淋巴细胞%受体,白细胞介素2%受体,白细胞介素7%流式细胞术
肝炎病毒,乙型%T淋巴細胞%受體,白細胞介素2%受體,白細胞介素7%流式細胞術
간염병독,을형%T림파세포%수체,백세포개소2%수체,백세포개소7%류식세포술
Hepatitis B virus%T-lymphocyes%Receptors,interleukin-2%Receptors,interleukin-7%Flow cytometry
目的 检测乙型肝炎病毒( HBV)感染者外周血CD4+T细胞表面CD25 - CD127 -的表达情况及临床意义.方法 用流式细胞术比较分析53例慢性乙肝患者、53例HBV携带者和26例正常对照人群CD4+T细胞表面CD25 - CD127 -的表达情况,并对20例用干扰素治疗的HBV-DNA阳性慢性乙肝患者随访.结果 ①与正常对照组比较,慢性乙肝患者、HBV携带者CD4+ CD25 -CD127 -T细胞均显著升高,两者比较有统计学差异(Q =4.559,P＜0.05；Q=6.230,P＜0.05)；②HBV- DNA阳性患者(n=77) CD4+ CD25 - CD127 -T细胞显著低于HBV- DNA阴性患者(n=29),两者比较有统计学意义(t =2.290,P=0.024)；③与治疗前比,慢性乙肝患者干扰素治疗12周后CD4+CD25- CD127 -T细胞显著降低,两者比较差异有统计学意义(t =2.469,P=0.024).结论 乙型肝炎病毒感染者外周血CD4+ CD25 - CD127 -T细胞与病毒的感染和清除相关,外源性干扰素可降低CD4+ CD25 - CD127 -T细胞.
目的 檢測乙型肝炎病毒( HBV)感染者外週血CD4+T細胞錶麵CD25 - CD127 -的錶達情況及臨床意義.方法 用流式細胞術比較分析53例慢性乙肝患者、53例HBV攜帶者和26例正常對照人群CD4+T細胞錶麵CD25 - CD127 -的錶達情況,併對20例用榦擾素治療的HBV-DNA暘性慢性乙肝患者隨訪.結果 ①與正常對照組比較,慢性乙肝患者、HBV攜帶者CD4+ CD25 -CD127 -T細胞均顯著升高,兩者比較有統計學差異(Q =4.559,P＜0.05；Q=6.230,P＜0.05)；②HBV- DNA暘性患者(n=77) CD4+ CD25 - CD127 -T細胞顯著低于HBV- DNA陰性患者(n=29),兩者比較有統計學意義(t =2.290,P=0.024)；③與治療前比,慢性乙肝患者榦擾素治療12週後CD4+CD25- CD127 -T細胞顯著降低,兩者比較差異有統計學意義(t =2.469,P=0.024).結論 乙型肝炎病毒感染者外週血CD4+ CD25 - CD127 -T細胞與病毒的感染和清除相關,外源性榦擾素可降低CD4+ CD25 - CD127 -T細胞.
목적 검측을형간염병독( HBV)감염자외주혈CD4+T세포표면CD25 - CD127 -적표체정황급림상의의.방법 용류식세포술비교분석53례만성을간환자、53례HBV휴대자화26례정상대조인군CD4+T세포표면CD25 - CD127 -적표체정황,병대20례용간우소치료적HBV-DNA양성만성을간환자수방.결과 ①여정상대조조비교,만성을간환자、HBV휴대자CD4+ CD25 -CD127 -T세포균현저승고,량자비교유통계학차이(Q =4.559,P＜0.05；Q=6.230,P＜0.05)；②HBV- DNA양성환자(n=77) CD4+ CD25 - CD127 -T세포현저저우HBV- DNA음성환자(n=29),량자비교유통계학의의(t =2.290,P=0.024)；③여치료전비,만성을간환자간우소치료12주후CD4+CD25- CD127 -T세포현저강저,량자비교차이유통계학의의(t =2.469,P=0.024).결론 을형간염병독감염자외주혈CD4+ CD25 - CD127 -T세포여병독적감염화청제상관,외원성간우소가강저CD4+ CD25 - CD127 -T세포.
Objective To investigate the clinical significance and detection of the expression of CD25 - CD127 - on CD4 + T cells in peripheral blood in patients with hepatitis B.Methods The expression of CD25- CD127- on CD4 + T cells were measured by using flow cytometry in 53 patients with chronic hepatitis B,53 carrier with hepatitis B virus and 26 healthy blood donors,and follow up 20 patients with HBV-DNA positive treated with interferon.Results ① Compared with healthy controls,the expression of CD25 - CD127 - on CD4 + T cells in patients and carrier with hepatitis B virus were lower( Q =4.559,P ＜0.05 ; Q =6.230,P ＜ 0.05 ).②The expression of CD25 - CD127 - on CD4 + T cells in patients with HBV-DNA positive( n =77 ) was lower than that of negative ( n =29 ) ( t =2.290,P =0.024 ).③Compared with the prior treatment,the expression of CD25 - CD127 - on CD4 + T cells in patients with B hepatitis were lower after interferon treated with 12 weeks(t =2.469,P =0.024).Conclusion It suggested that the CD25-CD127- expression on CD4 + T cells correlated with viral infections and cleared,exogenous interferon could decrease CD25 - CD127 - expression on CD4 + T cells.
