中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2011年
5期
437-442
,共6页
陈倩%曹新%胡正%施圣云%张其华
陳倩%曹新%鬍正%施聖雲%張其華
진천%조신%호정%시골운%장기화
呼吸道感染%博卡病毒属%细小病毒科感染%聚合酶链反应%儿童
呼吸道感染%博卡病毒屬%細小病毒科感染%聚閤酶鏈反應%兒童
호흡도감염%박잡병독속%세소병독과감염%취합매련반응%인동
Respiratory tract infections%Bocavirus%Parvoviridae infections%Polymerase chain reaction%Child
目的 了解南京地区急性呼吸道感染患儿HBoV的检出情况,并探讨HBoV与临床特征的关系.方法 收集2009年7月至2010年6月南京医科大学附属南京儿童医院呼吸科收治的397例急性呼吸道感染住院患儿为病例组,对照组为50例无呼吸道感染症状的儿童.应用实时荧光定量PCR法检测鼻咽分泌物标本HBoV.对HBoV阳性标本,应用实时荧光定量PCR法检测MP和CT,直接免疫荧光法检测RSV、ADV、IVA、IVB、PIV-1、PIV-2、PIV-3和hMPV.随机选取5份HBoV阳性标本扩增HBoV NP-1片段后进行核苷酸序列测定,结果与GenBank中已知序列进行比对,绘制系统进化树.结合HBoV阳性患儿临床资料,分析HBoV的流行病学特点、临床表现和最终临床诊断.结果 实时荧光定量PCR法检出HBoV DNA阳性率为8.3%(33/397),其中有57.6%(19/33)混合其他病原体感染.与HBoV混合感染的前3位病原体依次为MP(27.3%,9/33)、RSV(24.2%,8/33)和PIV.3(12.1%,4/33).7～36个月龄感染HBoV患儿有25例,占HBoV DNA阳性患儿的75.8%(25/33).5份标本HBoV NP-1基因序列均一致,与st1、st2和WHL-1等序列同源性为99%～100%.结论 HBoV是南京地区急性呼吸道感染患儿的病原之一.HBoV NP-1基因高度保守,在不同地区和不同时期的流行株间变异较小,可作为实时荧光定量PCR等方法的检测标记.
目的 瞭解南京地區急性呼吸道感染患兒HBoV的檢齣情況,併探討HBoV與臨床特徵的關繫.方法 收集2009年7月至2010年6月南京醫科大學附屬南京兒童醫院呼吸科收治的397例急性呼吸道感染住院患兒為病例組,對照組為50例無呼吸道感染癥狀的兒童.應用實時熒光定量PCR法檢測鼻嚥分泌物標本HBoV.對HBoV暘性標本,應用實時熒光定量PCR法檢測MP和CT,直接免疫熒光法檢測RSV、ADV、IVA、IVB、PIV-1、PIV-2、PIV-3和hMPV.隨機選取5份HBoV暘性標本擴增HBoV NP-1片段後進行覈苷痠序列測定,結果與GenBank中已知序列進行比對,繪製繫統進化樹.結閤HBoV暘性患兒臨床資料,分析HBoV的流行病學特點、臨床錶現和最終臨床診斷.結果 實時熒光定量PCR法檢齣HBoV DNA暘性率為8.3%(33/397),其中有57.6%(19/33)混閤其他病原體感染.與HBoV混閤感染的前3位病原體依次為MP(27.3%,9/33)、RSV(24.2%,8/33)和PIV.3(12.1%,4/33).7～36箇月齡感染HBoV患兒有25例,佔HBoV DNA暘性患兒的75.8%(25/33).5份標本HBoV NP-1基因序列均一緻,與st1、st2和WHL-1等序列同源性為99%～100%.結論 HBoV是南京地區急性呼吸道感染患兒的病原之一.HBoV NP-1基因高度保守,在不同地區和不同時期的流行株間變異較小,可作為實時熒光定量PCR等方法的檢測標記.
목적 료해남경지구급성호흡도감염환인HBoV적검출정황,병탐토HBoV여림상특정적관계.방법 수집2009년7월지2010년6월남경의과대학부속남경인동의원호흡과수치적397례급성호흡도감염주원환인위병례조,대조조위50례무호흡도감염증상적인동.응용실시형광정량PCR법검측비인분비물표본HBoV.대HBoV양성표본,응용실시형광정량PCR법검측MP화CT,직접면역형광법검측RSV、ADV、IVA、IVB、PIV-1、PIV-2、PIV-3화hMPV.수궤선취5빈HBoV양성표본확증HBoV NP-1편단후진행핵감산서렬측정,결과여GenBank중이지서렬진행비대,회제계통진화수.결합HBoV양성환인림상자료,분석HBoV적류행병학특점、림상표현화최종림상진단.결과 실시형광정량PCR법검출HBoV DNA양성솔위8.3%(33/397),기중유57.6%(19/33)혼합기타병원체감염.여HBoV혼합감염적전3위병원체의차위MP(27.3%,9/33)、RSV(24.2%,8/33)화PIV.3(12.1%,4/33).7～36개월령감염HBoV환인유25례,점HBoV DNA양성환인적75.8%(25/33).5빈표본HBoV NP-1기인서렬균일치,여st1、st2화WHL-1등서렬동원성위99%～100%.결론 HBoV시남경지구급성호흡도감염환인적병원지일.HBoV NP-1기인고도보수,재불동지구화불동시기적류행주간변이교소,가작위실시형광정량PCR등방법적검측표기.
Objective To investigate the possible existence of HBoV in children with acute respiratory infections in Nanjing area and explore its relationship with clinical characteristics.Methods A total of 397 nasopharyngeal secretion samples were collected from children with acute respiratory infection,admitted from July 2009 to June 2010 in Nanjing Children'S Hospital affiliated to Nanjing Medical University,and 50 cases of children without symptoms of respiratory infection were recruited as control group,whose nasopharyngeal secretion samples were also collected.HBoV was determined by real-time fluorescence quantitative PCR.MP and CT were detected by real-time fluorescence quantitative PCR in those HBoV-positive samples.RSV,ADV,IVA,IVB,PIV-1,PIV-2,PIV-3 and hMPV were detected by direct antigen-specific immunofluorescence assays.HBoV NP-1 fragments were amplified and sequenced in 5 HBoV positive samples randomly selected.The results were compared with the known GenBank sequence,and thereby the phylogenetic tree was established.The epidemiological characteristics,clinical presentation and the final clinical diagnosis of HBoV were analyzed according to the clinical data of the HBoV-positive patients.Results Thirty-three HBoV-positive cases were detected by real-time fluorescence quantitative PCR method with a positivity rate of 8. 3% ( 33/397 ). Among the 33 HBoV-positive cases, 19 cases (57.6%) were multiple infections with HBoV and other pathogens, the top three of which were MP (27.3% ,9/33 ),RSV (24.2% , 8/33 ) and PIV-3 ( 12. 1% ,4/33 ). Affected children aged from 7 to 36 months old accounted for 75.8% of the total ( 25/33 ). The measured HBoV NP-1 gene sequences of 5 specimens were consistent,indicating a high homology (99% to 100% ) with the stl, st2 and WHL-1. Conclusions HBoV is one of the pathogens of children's acute respiratory infections in Nanjing. HBoV NP-1 gene is highly conserved,with little variation in different seasons and in different regions and therefore can be used as a marker for real-time fluorescence quantitative PCR and other methods.
