中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2010年
1期
38-41
,共4页
杨瑞芳%陈丽娟%李建勇%李春溟%许家仁%吴雨洁%陆化
楊瑞芳%陳麗娟%李建勇%李春溟%許傢仁%吳雨潔%陸化
양서방%진려연%리건용%리춘명%허가인%오우길%륙화
多发性骨髓瘤%骨髓微环境%miR-21%miR-30b
多髮性骨髓瘤%骨髓微環境%miR-21%miR-30b
다발성골수류%골수미배경%miR-21%miR-30b
Multiple myeloma%Microenvironment%miR-21%miR-30b
目的 研究骨髓微环境对多发性骨髓瘤(MM)细胞mieroRNA(miR)-21和miR-30b的调变作用.方法培养破骨细胞,采用MACS法分选纯化CD138*骨髓瘤细胞.采用实时定量PCR的方法检测MM患者瘤细胞和细胞株以及与破骨细胞共培养的MM患者瘤细胞miR-21和miR-30b的表达量.结果 MM患者的外周血单个核细胞经破骨细胞培养基培养后10～14 d形成破骨细胞,瘤细胞与破骨细胞共培养组和未与破骨细胞共培养组相比较,细胞活力明显增高,miR-21的表达量增高了1.3～5.9倍,miR-30b表达量降低27.5%～76.9%.在正常浆细胞、MM患者瘤细胞和MM细胞株,miR-21表达水平分别为1.9±0.8、6.5±4.9和35.1±36.2,而miR-30表达分别为13.6±1.8、7.2±6.3和4.5±1.9.结论骨髓微环境可调控miR-21和miR-30b的表达,miR-21起癌基因作用,而miR-30b起抑痛基因作用.
目的 研究骨髓微環境對多髮性骨髓瘤(MM)細胞mieroRNA(miR)-21和miR-30b的調變作用.方法培養破骨細胞,採用MACS法分選純化CD138*骨髓瘤細胞.採用實時定量PCR的方法檢測MM患者瘤細胞和細胞株以及與破骨細胞共培養的MM患者瘤細胞miR-21和miR-30b的錶達量.結果 MM患者的外週血單箇覈細胞經破骨細胞培養基培養後10～14 d形成破骨細胞,瘤細胞與破骨細胞共培養組和未與破骨細胞共培養組相比較,細胞活力明顯增高,miR-21的錶達量增高瞭1.3～5.9倍,miR-30b錶達量降低27.5%～76.9%.在正常漿細胞、MM患者瘤細胞和MM細胞株,miR-21錶達水平分彆為1.9±0.8、6.5±4.9和35.1±36.2,而miR-30錶達分彆為13.6±1.8、7.2±6.3和4.5±1.9.結論骨髓微環境可調控miR-21和miR-30b的錶達,miR-21起癌基因作用,而miR-30b起抑痛基因作用.
목적 연구골수미배경대다발성골수류(MM)세포mieroRNA(miR)-21화miR-30b적조변작용.방법배양파골세포,채용MACS법분선순화CD138*골수류세포.채용실시정량PCR적방법검측MM환자류세포화세포주이급여파골세포공배양적MM환자류세포miR-21화miR-30b적표체량.결과 MM환자적외주혈단개핵세포경파골세포배양기배양후10～14 d형성파골세포,류세포여파골세포공배양조화미여파골세포공배양조상비교,세포활력명현증고,miR-21적표체량증고료1.3～5.9배,miR-30b표체량강저27.5%～76.9%.재정상장세포、MM환자류세포화MM세포주,miR-21표체수평분별위1.9±0.8、6.5±4.9화35.1±36.2,이miR-30표체분별위13.6±1.8、7.2±6.3화4.5±1.9.결론골수미배경가조공miR-21화miR-30b적표체,miR-21기암기인작용,이miR-30b기억통기인작용.
Objective To investigate the expression of miR-21 and miR-30b in multiple myeloma (MM).Methods Peripheral blood mononuclear cells from patients with MM were cultured at 2.5×10~6cells/ml in α-MEM supplemented with 10% of fetal bovine serum,antibiotics,RANKL(50 ng/ml),and macrophage colony-stimulating factor(25 ng/ml)for 10 to 14 days to obtain osteoclasts with bone-resorbing activity.Primary myeloma cells were purified from 12 MM patients.Of them,8 samples were cocultured with osteoclasts and 4 as noncocultured control.The expression of miR-21 and miR-30b was detected by real-time PCR.Results The viability of MM cells recovered from cocuhures was higher than those of noncocultured control.After cocultured with osteoclasts,primary myeloma cells from eight patients exhibited a 2.3-6.9 fold increase in miR-21 expression and 1.38-4.32-fold decrease in miR-30b expression compared with controls.In highly purified plasma cells from 3 healthy subjects,12 MM patients and 11 MM cell lines,the expression of miR-21 Was 1.9±0.8,6.5±4.9 and 35.1±36.2,respectively;the expression of miR-30b was 13.6±1.8,7.2±6.3 and 4.5±1.9,respectively.Conclusions miR-21 acts as an oncogene and miR-30b a tumor suppressor gene in MM.
