CAJ | 학술논문

以分布于甘肃临泽平川乡的两种芦苇生态型--水生芦苇(水芦)和重度盐化草甸芦苇(盐芦)叶片为材料,研究了其抗氧化系统的特征.结果表明,与水芦相比,盐芦中未出现活性氧和MDA(丙二醛)的积累,抗氧化酶SOD(超氧化物歧化酶)、CAT(过氧化氢酶)、POD(过氧化物酶)和APX(抗坏血酸过氧化物酶)的活性显著升高.总抗坏血酸和类胡萝卜素含量在两种生态型芦苇中没有差异,但还原型抗坏血酸和总谷胱甘肽含量在盐芦中显著升高.而且,盐芦的LOX(脂氧合酶)活性比水芦低.这些结果表明,盐芦中有效的抗氧化防御系统对抵抗盐渍胁迫起着重要的作用.此外,盐芦中高活性的Ca2+- 和 Mg2+-ATPase对细胞中过多离子的转运以及避免离子毒害起着重要的作用.
이분포우감숙림택평천향적량충호위생태형--수생호위(수호)화중도염화초전호위(염호)협편위재료,연구료기항양화계통적특정.결과표명,여수호상비,염호중미출현활성양화MDA(병이철)적적루,항양화매SOD(초양화물기화매)、CAT(과양화경매)、POD(과양화물매)화APX(항배혈산과양화물매)적활성현저승고.총항배혈산화류호라복소함량재량충생태형호위중몰유차이,단환원형항배혈산화총곡광감태함량재염호중현저승고.이차,염호적LOX(지양합매)활성비수호저.저사결과표명,염호중유효적항양화방어계통대저항염지협박기착중요적작용.차외,염호중고활성적Ca2+- 화 Mg2+-ATPase대세포중과다리자적전운이급피면리자독해기착중요적작용.
Using plants possessing stable genetic tolerance/resistance under natural environmental conditions to study the mechanism of plants' adaptation to environmental stress are very significant. However, relevant work is very limited. In this work, two ecotypes of reed (Phragmites communis Trin.), referred to as "swamp reed (SR)" and "heavy salt meadow reed (HSMR)" according to their habitats, growing in Pingchuan Town, Linze County, Gansu Province (China) were investigated for the antioxidant system in their leaves. The results showed that compared with the SR, there were no accumulations of reactive oxygen species and MDA (malondialdehyde) in the HSMR. The activities of SOD (superoxide dismutase), CAT (catalase), POD (peroxidase) and APX (ascorbate peroxidase) were significantly higher in the HSMR than those in the SR. There were no differences in the contents of total ascorbic acid and carotenoids between the two ecotypes, while the contents of reduced ascorbic acid and total glutathione were higher in the HSMR than that in the SR. Moreover, the LOX (lipoxygenase) in the HSMR showed a decreased activity compared with the SR. These results suggested that more efficient antioxidant systems in the HSMR contribute to its tolerance / resistance to the saline stress. In addition, higher activities of membrane-bound Ca2+- and Mg2+-ATPase in the HSMR might contribute to transporting excessive ions out of cells, therefore avoiding ion toxicity when this reed ecotype grew in saline habitat.