CAJ | 학술논문

背景:星形细胞对神经元有提供营养、支持及调节突触活性作用,但它对神经元发育的影响还尚不清楚.目的:探讨体外培养的Sprague Dawley大鼠大脑皮质星形细胞对PC12神经元突起生长发育的作用.设计:完全随机设计,对照实验研究.方法:以培养的星形细胞与PC12神经元按不同细胞数目比例(50:1～1:1)共同培养,并用其制备的条件培养液培养PG12细胞.主要观察指标:用快速灵敏的MTT'比色法测定PC12神经元的细胞活力,用光学相差显微镜观察PC12细胞形态学变化.结果:①星形细胞条件培养液可增强PG12细胞活力(MTT测定的A值由0.255±0.012提高到0.510±0.036,P＜0.001),却不能促使PC12神经元突起的生出.②当将星形细胞与PC12细胞按30:1～1:1的比例共同培养时,既可提高PC12细胞折光性和光晕又可促使其突起的生长;但按50:1～40:1的比例共同培养时,只观察到提高PC12细胞折光性和光晕,而无促使其突起生长发育的作用.结论:PC12神经元细胞活力的提高与星形细胞分泌到条件培养液中的可溶性因子有关,而PC12神经元突起生长发育可能是和与星形细胞的直接接触以及二者的细胞数目比有关.
배경:성형세포대신경원유제공영양、지지급조절돌촉활성작용,단타대신경원발육적영향환상불청초.목적:탐토체외배양적Sprague Dawley대서대뇌피질성형세포대PC12신경원돌기생장발육적작용.설계:완전수궤설계,대조실험연구.방법:이배양적성형세포여PC12신경원안불동세포수목비례(50:1～1:1)공동배양,병용기제비적조건배양액배양PG12세포.주요관찰지표:용쾌속령민적MTT'비색법측정PC12신경원적세포활력,용광학상차현미경관찰PC12세포형태학변화.결과:①성형세포조건배양액가증강PG12세포활력(MTT측정적A치유0.255±0.012제고도0.510±0.036,P＜0.001),각불능촉사PC12신경원돌기적생출.②당장성형세포여PC12세포안30:1～1:1적비례공동배양시,기가제고PC12세포절광성화광훈우가촉사기돌기적생장;단안50:1～40:1적비례공동배양시,지관찰도제고PC12세포절광성화광훈,이무촉사기돌기생장발육적작용.결론:PC12신경원세포활력적제고여성형세포분비도조건배양액중적가용성인자유관,이PC12신경원돌기생장발육가능시화여성형세포적직접접촉이급이자적세포수목비유관.
BACKGROUND: Although astrocytes are kown to provide structural andtrophic support to neurons and modulate synaptic activity, their role is farfrom being completely understood.OBJECTIVE: To investigate effects of cultured astrocytes fromSprague-Dawley rat cerebral cortex on the neurite development of PC12 cellsderived from rat pheochromocytoma.DESIGN: Completely randomized controlled trial.METHODS: PC12 cells were co-cultured with astrocyte according to dif-ferent astrocytes/neurons ratio(50: 1 -1: 1), or cultured with serum-freeconditioned medium of astrocytes (ACM).MAIN OUTCOME MEASURES: The vitality of PC12 cells was measuredby sensitive MTT method and their morphologic features were observed byOlympus light microscope.RFSULTS: When PC12 cells were cultured with ACM, compared with thecontrol group, the vitality of PC12 cells was increased significantly(0. 255 ± 0. 012 vs 0. 510 ± 0. 036, P ＜ 0. 001 ) , but the neurite growthwas not obvious in the experimental group. When PC12 cells wereco-cultured with astrocyte in the ratio of 30:1 - 1: 1, not only was thevitality of PC12 cells enhanced, but also the neurite-outgrowth of PC12was observed. However, when PC12 cells were co-cultured with astrocyte inproportion of 50:10 -40: 1, the vitality of PC12 cells was also enhanced,but the neurite-outgrowth of PC12 was not found.CONCLUSION: This study suggested enhancement of PC12 cell-vitality wasmediated by soluble factors produced by astrocytes, while activity of theneurite-promoting was associated with cell-cell contact and with the ratio oftwo cells.