国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2008年
5期
409-411
,共3页
宋克征%徐朝阳%王峰%冯家飞
宋剋徵%徐朝暘%王峰%馮傢飛
송극정%서조양%왕봉%풍가비
果糖胺%方法%糖尿病%临床实验室技术
果糖胺%方法%糖尿病%臨床實驗室技術
과당알%방법%당뇨병%림상실험실기술
Fructosamine%Methods%Diabetes mellitus%Clinical laboratory techniques
目的 对NBT法测定血清果糖胺进行改良.方法 针对该法存在的主要问题:校正液的基质效应,还原性物质对测定的干扰,脂浊及NBT的析出等导致体系混浊和不稳定提出了对策,并取得了满意的效果.结果 通过对校正液生产工艺的改进,克服了基质效应,用2点速率法并将读数点尽量后延,消除了各种干扰.利用优选的非离子和阴离子表面活性剂,解决了体系的混浊和不稳定.经方法学评价,批内CV 0.9%,日间CV 2.9%,线性范围在0～6 mmol/L,参考值范围为1.58~2.14 mmol/L,试剂稳定1年.经临床核对6个批次共507例次的空腹血糖与果糖胺,结果完全符合.又观察了初诊确诊的糖尿病患者10例,核对了其果糖胺与空腹血糖和糖化血红蛋白的测定值,结果也完全符合.结论 经改进的NBT法操作简便、试剂稳定、符合临床,适用于血清果糖胺的常规测定.
目的 對NBT法測定血清果糖胺進行改良.方法 針對該法存在的主要問題:校正液的基質效應,還原性物質對測定的榦擾,脂濁及NBT的析齣等導緻體繫混濁和不穩定提齣瞭對策,併取得瞭滿意的效果.結果 通過對校正液生產工藝的改進,剋服瞭基質效應,用2點速率法併將讀數點儘量後延,消除瞭各種榦擾.利用優選的非離子和陰離子錶麵活性劑,解決瞭體繫的混濁和不穩定.經方法學評價,批內CV 0.9%,日間CV 2.9%,線性範圍在0～6 mmol/L,參攷值範圍為1.58~2.14 mmol/L,試劑穩定1年.經臨床覈對6箇批次共507例次的空腹血糖與果糖胺,結果完全符閤.又觀察瞭初診確診的糖尿病患者10例,覈對瞭其果糖胺與空腹血糖和糖化血紅蛋白的測定值,結果也完全符閤.結論 經改進的NBT法操作簡便、試劑穩定、符閤臨床,適用于血清果糖胺的常規測定.
목적 대NBT법측정혈청과당알진행개량.방법 침대해법존재적주요문제:교정액적기질효응,환원성물질대측정적간우,지탁급NBT적석출등도치체계혼탁화불은정제출료대책,병취득료만의적효과.결과 통과대교정액생산공예적개진,극복료기질효응,용2점속솔법병장독수점진량후연,소제료각충간우.이용우선적비리자화음리자표면활성제,해결료체계적혼탁화불은정.경방법학평개,비내CV 0.9%,일간CV 2.9%,선성범위재0～6 mmol/L,삼고치범위위1.58~2.14 mmol/L,시제은정1년.경림상핵대6개비차공507례차적공복혈당여과당알,결과완전부합.우관찰료초진학진적당뇨병환자10례,핵대료기과당알여공복혈당화당화혈홍단백적측정치,결과야완전부합.결론 경개진적NBT법조작간편、시제은정、부합림상,괄용우혈청과당알적상규측정.
Objective To modify nitroblue tetrazolium (NBT) reductive method for serum fructosamine (FMN) assay.Methods NBT assay has such principle problems as follows: matrix effects of calibration solution;interference from materials in samples;system clouding and unstablity due to lipid turbidity and NBT salting out in the system. Aiming at these problems,the corresponding strategy was introduced,which acquired a satisfied effect.Results The matrix effects of the calibrators have been overcame. And all other interferences were eliminated by using two point rate method and retardation of the window time. Turbidity of the system was omitted by using optimized anionic and nonionic detergents combined with salts of strong acid. Methodology evaluation: intra- and inter-assay precision CV were 0.9% and 2.9% respectively. Linearity range: 0-6 mmol/L;Assay rang: 1.58-2.14 mmol/L. The stability duration of the reagents was one year. In the clinical research trial,the level of FMN in 507 cases among 6 lots in two hospitals completely accorded with that of their respective fasting glucose. And at the same time,10 initially diagnosed diabetes mellitus (DM) patients were observed. Their FMN also accorded with respective fasting glucose,and so did HbA1c.Conclusion The modified novel method is simple and stable,suitable for clinical routine measurement of serum FMN.
