CAJ | 학술논문

目的观察人参皂甙Rb1针剂(Ginsenoside Rb1,GRb1)对腹腔感染大鼠肠黏膜细胞热休克蛋白-70(HSP-70)的影响,探讨GRbl对肠黏膜屏障的保护作用机制.方法 30只SD雄性大鼠随机分为正常组、对照组及实验组,各10只.对照组及实验组建立腹腔感染模型.实验组于造模前7d予GRb1针剂(20mg/kg),其他组予等量生理盐水,每天腹腔注射1次.各组造模后42 h,管饲已标记的大肠杆菌.48 h处死后,取小肠作病理学分析及免疫组织化学检测小肠黏膜HSP.70的表达;取胰腺、肾脏、脾脏、肝脏组织,检测大鼠移位标记菌变化.结果与对照组相比,GRb1能明显提高腹腔感染大鼠小肠黏膜的HSP-70表达,并减轻小肠黏膜组织破坏程度,减少胰腺、肾脏、脾脏、肝脏等周围器官的细菌移位.结论 GRb1能提高肠黏膜细胞HSP-70的表达,减轻其肠黏膜损伤,减少细菌移位,具有一定的肠黏膜屏障保护作用.
목적 관찰인삼조대Rb1침제(Ginsenoside Rb1,GRb1)대복강감염대서장점막세포열휴극단백-70(HSP-70)적영향,탐토GRbl대장점막병장적보호작용궤제.방법 30지SD웅성대서수궤분위정상조、대조조급실험조,각10지.대조조급실험조건립복강감염모형.실험조우조모전7d여GRb1침제(20mg/kg),기타조여등량생리염수,매천복강주사1차.각조조모후42 h,관사이표기적대장간균.48 h처사후,취소장작병이학분석급면역조직화학검측소장점막HSP.70적표체;취이선、신장、비장、간장조직,검측대서이위표기균변화.결과 여대조조상비,GRb1능명현제고복강감염대서소장점막적HSP-70표체,병감경소장점막조직파배정도,감소이선、신장、비장、간장등주위기관적세균이위.결론 GRb1능제고장점막세포HSP-70적표체,감경기장점막손상,감소세균이위,구유일정적장점막병장보호작용.
Objective Exploration of enhancing intestinal mucosa cells HSP70 expression can protect intest-inal mucosal barrier. Methods 30 SD male rats were randomly divided into the normal group, the control group and the treatment group. The latter 2 groups were supposed to be establishment of abdominal testis model. In the seven days before the start of the day by intraperitoneal injection once a treatment group to ginsenoside Rb1 injec-tion, the other groups were given equal saline. After 42 hours of the establishment of abdominal testis model, ma-rked Bacterium coli were fed. After 48 hours, the rats were killed. Keep the small intestine for pathologic analysis and immunohistochemistry mucosa of heat shock protein 70 expression. Keep the pancreas, kidney, spleen, liver tissue to detect bacterial translocation. Results Compared to the control group, Ginsenoside Rbl can significan-tly increase intra-abdominal infection of the small intestinal expression of heat shock protein 70, and reduce the or-ganizational structure of the small intestinal damage and bacterial translocation of pancreas, kidney, spleen, liver. Conclusions Increasing the expression of HSP70, of the intestinal cells' can reduce the organizational structure of the small intestinal damage and bacterial translocation. It can protect the intestinal mucosal barrier.