分析化学
分析化學
분석화학
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2009年
11期
1611-1616
,共6页
环境激素%食品包装材料%非水毛细管电泳%化学发光
環境激素%食品包裝材料%非水毛細管電泳%化學髮光
배경격소%식품포장재료%비수모세관전영%화학발광
Environmental estrogens%food packaging material%nonaqueous capillary electrophoresis%chemiluminescence
建立食用包装材料中的双酚A、壬基酚等多种环境激素的非水毛细管电泳-化学发光分析方法.食品包装材料样品浸出物中的双酚A、烷基酚等环境激素经衍生剂DIB-Cl衍生后,经过非水毛细管电泳分离后,分别与过氧草酸酯化学发光反应体系作用,光信号经过光电倍增管接收放大后被检测.以雌二醇(17β-E2)为内标,以相对迁移时间定性,相对发光强度比定量,内标校准曲线法测定样品浸出物中待测物的含量.对影响非水毛细管电泳分离如溶剂组成和比例、电解质浓度、温度、乙酸浓度、电泳电压等条件进行了优化.同时对化学发光体系也进行了优化.4-叔丁基酚、双酚A、4-叔辛基酚、4-壬基酚和四溴双酚 A分别在0.0095～3.0 mg/L,0.0087～3.0 mg/L,0.0085～3.0 mg/L,0.011～3.0 mg/L和0.009～3.0 mg/L范围内线性良好,r＞0.9947.相对迁移时间和相对峰高的RSD分别为0.88%～2.96%和 6.54%～8.57%.加标回收率为86.7%～98.8%.对5种常见的食品包装材料样品进行了测定,所建立的方法简便、快速、灵敏,适合于食品包装材料中酚类环境激素的检测.
建立食用包裝材料中的雙酚A、壬基酚等多種環境激素的非水毛細管電泳-化學髮光分析方法.食品包裝材料樣品浸齣物中的雙酚A、烷基酚等環境激素經衍生劑DIB-Cl衍生後,經過非水毛細管電泳分離後,分彆與過氧草痠酯化學髮光反應體繫作用,光信號經過光電倍增管接收放大後被檢測.以雌二醇(17β-E2)為內標,以相對遷移時間定性,相對髮光彊度比定量,內標校準麯線法測定樣品浸齣物中待測物的含量.對影響非水毛細管電泳分離如溶劑組成和比例、電解質濃度、溫度、乙痠濃度、電泳電壓等條件進行瞭優化.同時對化學髮光體繫也進行瞭優化.4-叔丁基酚、雙酚A、4-叔辛基酚、4-壬基酚和四溴雙酚 A分彆在0.0095～3.0 mg/L,0.0087～3.0 mg/L,0.0085～3.0 mg/L,0.011～3.0 mg/L和0.009～3.0 mg/L範圍內線性良好,r＞0.9947.相對遷移時間和相對峰高的RSD分彆為0.88%～2.96%和 6.54%～8.57%.加標迴收率為86.7%～98.8%.對5種常見的食品包裝材料樣品進行瞭測定,所建立的方法簡便、快速、靈敏,適閤于食品包裝材料中酚類環境激素的檢測.
건립식용포장재료중적쌍분A、임기분등다충배경격소적비수모세관전영-화학발광분석방법.식품포장재료양품침출물중적쌍분A、완기분등배경격소경연생제DIB-Cl연생후,경과비수모세관전영분리후,분별여과양초산지화학발광반응체계작용,광신호경과광전배증관접수방대후피검측.이자이순(17β-E2)위내표,이상대천이시간정성,상대발광강도비정량,내표교준곡선법측정양품침출물중대측물적함량.대영향비수모세관전영분리여용제조성화비례、전해질농도、온도、을산농도、전영전압등조건진행료우화.동시대화학발광체계야진행료우화.4-숙정기분、쌍분A、4-숙신기분、4-임기분화사추쌍분 A분별재0.0095～3.0 mg/L,0.0087～3.0 mg/L,0.0085～3.0 mg/L,0.011～3.0 mg/L화0.009～3.0 mg/L범위내선성량호,r＞0.9947.상대천이시간화상대봉고적RSD분별위0.88%～2.96%화 6.54%～8.57%.가표회수솔위86.7%～98.8%.대5충상견적식품포장재료양품진행료측정,소건립적방법간편、쾌속、령민,괄합우식품포장재료중분류배경격소적검측.
Nonaqueous capillary electrophoresis-chemiluminescence (NACE) method was developed for the determination of five phenolic environmental estrogens including bisphenol A (BPA),4-nonylphenol(4-NP) ,4-tert-octylphenol(4-tOP),4-tert-butylphenol(4-tBP) and tetrabromobisphenol A(TBBPA) in food packaging material. Target compounds in soaking solution of food packaging material samples were separated by NACE-CL after derivatization with 4-(4,5-diphenyl-lH-imidazol-2-yl) benzoyl chloride ( DIB-Cl) ,reacted with per-oxyoxalate chemiluminescence system,and then detected through magnifying light signal with photomultiplier. With 17β-E_2 as internal standard,qualitative and quantitative analysis of the target compounds were performed by relative migration time and the ratio of relative chemiluminescence intensity,respectively. Several influence factors on separation with NACE,such as the composition and proportion of organic solvent,the concentration of electrolyte,temperature surrounding of the capillary,the concentration of acetic acid and work voltage,were investigated. And the peroxyoxalate chemiluminescence system was optimized. Under these conditions,4-tBP,BPA,4-OP,4-NP and TBBPA were all separated and showed good linearities in the ranges of 0.0095-3.0 mg/L,0.0087-3.0 mg/L,0.0085 -3.0 mg/L,0.011 -3.0 mg/L,0.009-3.0 mg/L,respectively,with correlation coefficients over 0. 9947. The RSDs of relative migration time and relative chemiluminescence intensity ratio were 0.9% -3.0% and 6.5% - 8.6%. Recoveries for the spiked samples ranged from 86.7% to 98. 8%. Five common food packaging material samples were analyzed. The proposed method is simple and sensitive for the quantitative determination of phenolic environmental estrogens in food packaging material samples.
