CAJ | 학술논문

研究双水相萃取橙皮苷的成相体系及影响萃取率的因素.通过试验研究选择丙酮与(NH_4)_2SO_4-H_2O为双水相体系,研究盐用量、pH值及粗提物的用晕对萃取率的影响.结果表明:在90%内酮与(NH_4)_2SO_4溶液体积各为10ml的情况下,(NH_4)_2SO_4用量4.0g、样品0.35g、pH4的条件下,萃取效率最佳.经萃取,橘皮中的橙皮苷主要进入有机相中,萃取率为98.22%.通过薄层分析,在365nm紫外灯下,上相溶液与标准品的斑点Rf值接近,下相溶液检测不到橙皮苷.
연구쌍수상췌취등피감적성상체계급영향췌취솔적인소.통과시험연구선택병동여(NH_4)_2SO_4-H_2O위쌍수상체계,연구염용량、pH치급조제물적용훈대췌취솔적영향.결과표명:재90%내동여(NH_4)_2SO_4용액체적각위10ml적정황하,(NH_4)_2SO_4용량4.0g、양품0.35g、pH4적조건하,췌취효솔최가.경췌취,귤피중적등피감주요진입유궤상중,췌취솔위98.22%.통과박층분석,재365nm자외등하,상상용액여표준품적반점Rf치접근,하상용액검측불도등피감.
An aqueous two-phase system consisting of acetone, water and ammonium sulfate was developed for the purification of crude hesperidin extracted from orange peel using aqueous sodium carbonate solution. Effects of amount of ammonium sulfate addition, amount of crude hesperidin and pH on hesperidin extraction were studied. Results showed that an optimum extraction rate of hesperidin as high as 98.22% was obtained when the aqueous two-phase system consisting of 10 ml of 90% aqueous acetone and 10 ml of water containing 4.0 g of ammonium sulfate at pH 4 was used for treating 0.35 g of crude hesperidin. Thin layer chromatographic (TLC) analysis indicated that hesperidin was mainly partitioned into acetone phase with a Rf value close to that of hesperidin standard.