CAJ | 학술논문

目的了解噬菌体在不同采样液中耐冲击能力的强弱,筛选用于液体冲击式采样器采集噬菌体气溶胶的最佳采样液和耐冲击性强的噬菌体.方法使用全玻璃液体冲击采样器(AGI-10)对噬菌体进行耐冲击实验,采样液选用蒸馏水(DW)、磷酸盐缓冲液(PBS)和SM液,每种采样液又分为加橄榄油组(50 μl)和不加橄榄油组(0 μl),在以7 L/min的气流冲击30 min后测定采样液中噬菌体滴度和终末采样液体积,采用校正存活率评价噬菌体耐冲击性.结果同一种噬菌体在不同采样液中的耐冲击性不同,以SM液作为采样液时,噬菌体存活率较高,SM液中是否加入橄榄油对噬菌体的耐冲击性没有影响.噬菌体SM701、SM702、PhiX174、EcP1和F2在SM液中经7 L/min的气流冲击60 min后,校正存活率分别约为79%、77%、86%、50%和71%.结论噬菌体在不同采样介质中的耐冲击性不同,其中以SM液保护效果最佳,噬菌体SM701、SM702和PhiX174的耐冲击性较强.
목적 료해서균체재불동채양액중내충격능력적강약,사선용우액체충격식채양기채집서균체기용효적최가채양액화내충격성강적서균체.방법 사용전파리액체충격채양기(AGI-10)대서균체진행내충격실험,채양액선용증류수(DW)、린산염완충액(PBS)화SM액,매충채양액우분위가감람유조(50 μl)화불가감람유조(0 μl),재이7 L/min적기류충격30 min후측정채양액중서균체적도화종말채양액체적,채용교정존활솔평개서균체내충격성.결과 동일충서균체재불동채양액중적내충격성불동,이SM액작위채양액시,서균체존활솔교고,SM액중시부가입감람유대서균체적내충격성몰유영향.서균체SM701、SM702、PhiX174、EcP1화F2재SM액중경7 L/min적기류충격60 min후,교정존활솔분별약위79%、77%、86%、50%화71%.결론 서균체재불동채양개질중적내충격성불동,기중이SM액보호효과최가,서균체SM701、SM702화PhiX174적내충격성교강.
Objective To investigate the endurance or resistance of different bacteriophages to bubbling stress in different sampling solutions,to select the optimum sampling solution from three different ones and to select relatively stress-resistant bacteriophages from five different ones.Methods AGI-10(all glass impinger)was used as the representative for all the impingers that would bubble during operation to fulfill the bubbling experiment.Three different sampling solutions used,such as distilled water(DW),phosphatic buffer solution(PBS),and suspension medium(SM),were divided into two groups by adding olive oil(50 μl) or otherwise(0 μl).The impingers were operated 30 min at a flow rate of 7.0 L/min.The titers of bacteriophages and the volume of final sampling solutions were determined before the corrected survival probability was used to evaluate the stress resistance of several different bacteriophages.Results It was found that the survival probability of the same bacteriophage bubbling with different sampling solutions was different except for bacteriophage F2.The use of SM as the collection fluid was related to a high survival probability which remained unchanged between 50 μl and 0 μl olive oil.The corrected survival probability was 79%,77%,86%,50% and 71% for phage SM701,SM702,PhiX174,EcP1 and F2 respectively after 60 minutes of impingement at a flow rate of 7.0 L/min.Conclusion The endurance or resistance of different kinds of bacteriophages in the same sampling solution is different.SM might be an optimum sampling solution for phages.Bacteriophage SM701,SM702 and PhiX174 are more resistant to bubbling stress than EcP1 and F2.