华中科技大学学报（医学版）
華中科技大學學報（醫學版）
화중과기대학학보（의학판）
ACTA UNIVERSITATIS MEDICINAE TONGJI
2009年
6期
793-795
,共3页
张永红%于冬冬%何乐亚%魏欣%冷彦%陶德定%胡俊波%龚建平
張永紅%于鼕鼕%何樂亞%魏訢%冷彥%陶德定%鬍俊波%龔建平
장영홍%우동동%하악아%위흔%랭언%도덕정%호준파%공건평
异时相表达%细胞分裂周期%外周血淋巴细胞
異時相錶達%細胞分裂週期%外週血淋巴細胞
이시상표체%세포분렬주기%외주혈림파세포
unscheduled expression%cell division cycle%peripheral blood lymphocyte
目的 研究通常表达于G2/M期的Cyclin B1在G_1期异时相表达的作用机制及意义.方法 用丝裂原刺激因子(PHA)刺激人外周血淋巴细胞使之进入细胞周期,分别在刺激后0、36、48、60 h收集细胞,并分成两部分,一部分用流式Cyclins/DNA多参数技术分析,一部分用Post-sorting Western blot分析.结果 处于G_0期的淋巴细胞经PHA刺激进入细胞周期后,可以观察到Cyclin B1在G_1期有异时相表达,相应的CDK1亦有异时相表达.结论 异时相Cyclin B1/CDK1可能有助于正常在体细胞进入细胞分裂周期.
目的 研究通常錶達于G2/M期的Cyclin B1在G_1期異時相錶達的作用機製及意義.方法 用絲裂原刺激因子(PHA)刺激人外週血淋巴細胞使之進入細胞週期,分彆在刺激後0、36、48、60 h收集細胞,併分成兩部分,一部分用流式Cyclins/DNA多參數技術分析,一部分用Post-sorting Western blot分析.結果 處于G_0期的淋巴細胞經PHA刺激進入細胞週期後,可以觀察到Cyclin B1在G_1期有異時相錶達,相應的CDK1亦有異時相錶達.結論 異時相Cyclin B1/CDK1可能有助于正常在體細胞進入細胞分裂週期.
목적 연구통상표체우G2/M기적Cyclin B1재G_1기이시상표체적작용궤제급의의.방법 용사렬원자격인자(PHA)자격인외주혈림파세포사지진입세포주기,분별재자격후0、36、48、60 h수집세포,병분성량부분,일부분용류식Cyclins/DNA다삼수기술분석,일부분용Post-sorting Western blot분석.결과 처우G_0기적림파세포경PHA자격진입세포주기후,가이관찰도Cyclin B1재G_1기유이시상표체,상응적CDK1역유이시상표체.결론 이시상Cyclin B1/CDK1가능유조우정상재체세포진입세포분렬주기.
Objective To study the mechanism of unscheduled Cyclin B1 expression at G_1 phase which is usually at G_2/M phase.Methods Human peripheral blood lymphocytes(PBL) from healthy volunteers were firstly activated by PHA and then went into cell cycle.The cells were collected at 0,36,48 and 60 h after activation and divided into two parts:one for Cyclins/DNA muhiparameter assay,and another for Post-sorting Western blot.Results After activation by PHA,Cyclin B1 and CDK1 of lymphocytes were expressed at G_1 phase.Conclusion Unscheduled Cyclin B1/CDKl probably contributes to lymphocytes in vivo into cell cycle.
