CAJ | 학술논문

利用四乙氧基硅烷(TEOS)原位水解法将SiO_2掺杂于海藻酸(ALG)凝胶中,通过双交联制备出新型ALG-SiO_2杂化凝胶以固定化洋葱伯克霍尔德菌脂肪酶.结果表明,固定化酶的最优条件:质量分数为2.0%的ALG、0.2 mol/L CaCl2、V(ALG)/V(TEOS)为5、加酶量为1 g ALG 加100 mg酶粉、固定化60 min、采用直径为0.8 mm的针头滴定、真空冷冻干燥.在此条件下,酶蛋白的包埋率可达100%,酶活回收率可达91%.固定化酶的最适 pH 为 8.0,最适作用温度为50 ℃,重复使用8次后,酶活性仍能保持80%以上.ALG-SiO_2杂化凝胶的场扫描电镜(FESEM)观察发现凝胶的整体构造仍然是海藻酸凝胶骨架;与ALG凝胶平滑的内部相比较,杂化凝胶仍具有完整的网络结构,但内部更为粗糙,结构更为致密.
이용사을양기규완(TEOS)원위수해법장SiO_2참잡우해조산(ALG)응효중,통과쌍교련제비출신형ALG-SiO_2잡화응효이고정화양총백극곽이덕균지방매.결과표명,고정화매적최우조건:질량분수위2.0%적ALG、0.2 mol/L CaCl2、V(ALG)/V(TEOS)위5、가매량위1 g ALG 가100 mg매분、고정화60 min、채용직경위0.8 mm적침두적정、진공냉동간조.재차조건하,매단백적포매솔가체100%,매활회수솔가체91%.고정화매적최괄 pH 위 8.0,최괄작용온도위50 ℃,중복사용8차후,매활성잉능보지80%이상.ALG-SiO_2잡화응효적장소묘전경(FESEM)관찰발현응효적정체구조잉연시해조산응효골가;여ALG응효평활적내부상비교,잡화응효잉구유완정적망락결구,단내부경위조조,결구경위치밀.
Lipase from Burkholderia cepacia was entrapped in alginate-silica (ALG-SiO_2) hybrid gel prepared by hydrolysis of tetraethoxy silicone (TEOS) into alginate gel by double-cross-linking method. The optimal conditions were as follows: the concentration of sodium alginate and CaCl2 enzyme load was 100 mg/g carrier, immobilization time was 60 min, using 0.8 mm diameter needle for titration, and vacuum freeze-dried. Under the optimal conditions, the immobilization efficiency reached 100%, and the activity recovery was 91%. ALG-SiO_2 hybrid gel observed by field scanning electron microscope showed that the overall gel structure remained alginate gel skeleton, many small particles distributed inside the ALG-SiO_2 hybrid gel. ALG-SiO_2 hybrid gel interface appeared coarser and the overall structure becoming more compact as compared with ALG gel.