遗传
遺傳
유전
HEREDITAS(BEIJING)
2010年
2期
153-162
,共10页
蒋文枰%李家乐%郑润玲%汪桂玲
蔣文枰%李傢樂%鄭潤玲%汪桂玲
장문평%리가악%정윤령%왕계령
褶纹冠蚌%线粒体基因组%序列分析%ATP8基因%系统进化
褶紋冠蚌%線粒體基因組%序列分析%ATP8基因%繫統進化
습문관방%선립체기인조%서렬분석%ATP8기인%계통진화
Cristaria plicata%mitochondrial genome%sequence analysis%ATP8 gene%phylogeny
采用LA-PCR(Long amplification polymerase chain reaction)扩增方法首次获得褶纹冠蚌(Cristaria plicata)线粒体基因组全序列.分析表明:序列全长15712 bp,包括13个蛋白质基因、22个tRNA基因、2个rRNA基因和26个长度为2～328 bp的非编码区.A、T、C、G碱基组成分别为36.54%、27.22%、23.22%、13.02%.大部分基因在L链编码,其中ND3-ND5、ND4L、COⅠ～COⅢ、ATP6、ATP8、tRNA~(Asp)和tRNA~(His)在H链编码.基因排列与同科的射线佩饰真珠蚌(Lampsilis ornam)一致,与三角帆蚌(Hyriopsis cumingii)在COⅡ和12S rRNA之间存在差异.13个蛋白质基因具有I(AUU,AUC)、V(GUG)、M(AUA、AUG)3种起始密码子,除ND2终止密码子为不完整的T,其余基因均为典型的UAA或UAG.22个tRNA中,除tRNA~(Thr)、tRNA~(Lys)、tRNA~(Ser)(UCN)、tRNA~(Asp)、tRNA~(Arg)、tRNA~(Tyr)和tRNA~(Met)之外,其他15个tRNA都具有典型三叶草结构.与其他淡水双壳贝类一样,褶纹冠蚌具有ATP8基因,该基因可能与细胞质的渗透压平衡有关.
採用LA-PCR(Long amplification polymerase chain reaction)擴增方法首次穫得褶紋冠蚌(Cristaria plicata)線粒體基因組全序列.分析錶明:序列全長15712 bp,包括13箇蛋白質基因、22箇tRNA基因、2箇rRNA基因和26箇長度為2～328 bp的非編碼區.A、T、C、G堿基組成分彆為36.54%、27.22%、23.22%、13.02%.大部分基因在L鏈編碼,其中ND3-ND5、ND4L、COⅠ～COⅢ、ATP6、ATP8、tRNA~(Asp)和tRNA~(His)在H鏈編碼.基因排列與同科的射線珮飾真珠蚌(Lampsilis ornam)一緻,與三角帆蚌(Hyriopsis cumingii)在COⅡ和12S rRNA之間存在差異.13箇蛋白質基因具有I(AUU,AUC)、V(GUG)、M(AUA、AUG)3種起始密碼子,除ND2終止密碼子為不完整的T,其餘基因均為典型的UAA或UAG.22箇tRNA中,除tRNA~(Thr)、tRNA~(Lys)、tRNA~(Ser)(UCN)、tRNA~(Asp)、tRNA~(Arg)、tRNA~(Tyr)和tRNA~(Met)之外,其他15箇tRNA都具有典型三葉草結構.與其他淡水雙殼貝類一樣,褶紋冠蚌具有ATP8基因,該基因可能與細胞質的滲透壓平衡有關.
채용LA-PCR(Long amplification polymerase chain reaction)확증방법수차획득습문관방(Cristaria plicata)선립체기인조전서렬.분석표명:서렬전장15712 bp,포괄13개단백질기인、22개tRNA기인、2개rRNA기인화26개장도위2～328 bp적비편마구.A、T、C、G감기조성분별위36.54%、27.22%、23.22%、13.02%.대부분기인재L련편마,기중ND3-ND5、ND4L、COⅠ～COⅢ、ATP6、ATP8、tRNA~(Asp)화tRNA~(His)재H련편마.기인배렬여동과적사선패식진주방(Lampsilis ornam)일치,여삼각범방(Hyriopsis cumingii)재COⅡ화12S rRNA지간존재차이.13개단백질기인구유I(AUU,AUC)、V(GUG)、M(AUA、AUG)3충기시밀마자,제ND2종지밀마자위불완정적T,기여기인균위전형적UAA혹UAG.22개tRNA중,제tRNA~(Thr)、tRNA~(Lys)、tRNA~(Ser)(UCN)、tRNA~(Asp)、tRNA~(Arg)、tRNA~(Tyr)화tRNA~(Met)지외,기타15개tRNA도구유전형삼협초결구.여기타담수쌍각패류일양,습문관방구유ATP8기인,해기인가능여세포질적삼투압평형유관.
The complete mitochondrial genome of Cristaria plicata was obtained using long amplification polymerase chain reaction(LA-PCR).Analysis showed that the total length of sequence Was 15712 bp,including 13 protein-coding genes,2 ribosomal RNA genes,22 transfer RNA genes,and 26 non-coding regions ranged from 2 bp to 328 bp in size.The nucleotide composition of A,T, C,G were 36.54% 27.22% 23.22%and 13.02%,respectively.Most genes were encoded on the L strand,While ND3-ND5,ND4L,COⅠ-COⅢ,ATP6,ATP8,tRNA~(Asp),and tRNA~(His) were encoded on the H strand.The arrangement of genes in C. plicata was identical to that of Lampsilis ornata,but Was different from that of Hyriopsis cumingii between COⅡ and 12S rRNA in the same family.Thirteen protein genes contained 3 initiation codons,i.e.,I(AUU,AUC),V(GUG),and M(AUA,AUG),all of which had complete stop codons(UAA or UAG),except for the stop codon in ND2 that had an incomplete T.Fifteen tRNAs had typical cloverleaf structure,except for tRNA~(Thr),tRNA~(Lys),tRNA~(Ser)(UCN),tRNA~(Asp),tRNA~(Arg),tRNA~(Tyr),and tRNA~(Met).Like other freshwater bivalvia,C. plicata contained ATP8 gene,which might be related to the balance of osmotic pressure in the cytoplasm.
