CAJ | 학술논문

[目的]为探究钙离子、茉莉酸（Jasmonic acid,JA）对菠菜CBF转录因子表达的影响。[方法]以菠菜（Spinacia oleraceaL.）为材料,对其进行低温（4℃）或JA处理或用钙离子载体A23187预处理菠菜幼苗,利用Northern印迹技术检测各处理时CBF的表达情况。[结果]低温和JA均可诱导菠菜中CBF基因的表达。[结论]推测低温可能首先导致细胞中JA含量升高,JA通过一定的机制诱导细胞质基质中游离钙离子浓度（[Ca2＋]cyt）升高,然后Ca2＋可能通过CaM（Calmodulin）或CaM相关蛋白传递低温信号,从而诱导CBF的表达。
[목적]위탐구개리자、말리산（Jasmonic acid,JA）대파채CBF전록인자표체적영향。[방법]이파채（Spinacia oleraceaL.）위재료,대기진행저온（4℃）혹JA처리혹용개리자재체A23187예처리파채유묘,이용Northern인적기술검측각처리시CBF적표체정황。[결과]저온화JA균가유도파채중CBF기인적표체。[결론]추측저온가능수선도치세포중JA함량승고,JA통과일정적궤제유도세포질기질중유리개리자농도（[Ca2＋]cyt）승고,연후Ca2＋가능통과CaM（Calmodulin）혹CaM상관단백전체저온신호,종이유도CBF적표체。
[Objective] This experiment aimed to evaluate the effects of calcium and Jasmonic acid（JA） on the expression of CBF in spinach. [Methods] The seedlings of spinach were treated with low temperature （4 ℃）, JA or A23187, then used for detecting the expression of CBF by northern blotting. [Results] The results showed that the CBF expression was regulated by low temperature and JA positively. [Conclusions] Low temperature may increase the JA content of the cell firstly, then JA induced the increase of cytosolic calcium concentration （[Ca2＋]cyt）, and the JA induced Ca2＋ transmitted the low temperature signal through CaM or CaM related proteins, regulating the CBF expression.