CAJ | 학술논문

目的建立一种标准化的兔线栓法大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)模型,并探讨振幅整合脑电图(amplitude-integrated electroencephalogram,aEEG)对脑缺血判断的价值.方法 34只雄性新西兰兔随机分为MCAO组(n=29)和假手术组(n=5).线栓法制作MCAO模型,并行脑功能监测.根据2,3,5-三苯基四氮唑染色将MCAO组进一步分为皮质+基底节梗死组、基底节梗死组、蛛网膜下腔出血组和无病灶组,比较各亚组模型制作前后的生理指标、体重、栓线头端直径和插入长度以及aEEG之间的差异.结果兔线栓法MCAO模型制作成功率为62.07％(18/29),其中累及皮质和基底节者占37.93％(11/29),仅累及基底节者占24.38％(7/29),并发蛛网膜下腔出血者占17.24％(5/29),无梗死灶者占20.69％(6/29).各亚组之间模型制作前后体温、心率、平均动脉压以及动脉血pH、O2分压和CO2分压均无显著性差异.无病灶组体重为(2.36±0.10)kg,显著低于皮质+基底节梗死组的(2.55±0.09) kg(P=0.001)和基底节梗死组的(2.50±0.12)kg(P =0.017).皮质+基底节梗死组栓线置入深度为(5.59 ±0.24)cm,显著小于蛛网膜下腔出血组的(6.00 ±0.50)cm(P =0.036),但显著大于无病灶组的(5.20 ±0.50) cm(P =0.033).模型制作后各组aEEG之间存在显著差异(F=14.059,P=0.000),皮质+基底节梗死组和基底节梗死组造模后aEEG分别较模型制作前下降50.02％(=9.573,P＜0.001)和14.20％(=2.908,P=0.027).结论应用线栓法可成功建立标准化兔M CAO模型,aEEG显著降低提示MCAO模型制作成功,病灶累及皮质.
목적 건립일충표준화적토선전법대뇌중동맥전새(middle cerebral artery occlusion,MCAO)모형,병탐토진폭정합뇌전도(amplitude-integrated electroencephalogram,aEEG)대뇌결혈판단적개치.방법 34지웅성신서란토수궤분위MCAO조(n=29)화가수술조(n=5).선전법제작MCAO모형,병행뇌공능감측.근거2,3,5-삼분기사담서염색장MCAO조진일보분위피질+기저절경사조、기저절경사조、주망막하강출혈조화무병조조,비교각아조모형제작전후적생리지표、체중、전선두단직경화삽입장도이급aEEG지간적차이.결과 토선전법MCAO모형제작성공솔위62.07％(18/29),기중루급피질화기저절자점37.93％(11/29),부루급기저절자점24.38％(7/29),병발주망막하강출혈자점17.24％(5/29),무경사조자점20.69％(6/29).각아조지간모형제작전후체온、심솔、평균동맥압이급동맥혈pH、O2분압화CO2분압균무현저성차이.무병조조체중위(2.36±0.10)kg,현저저우피질+기저절경사조적(2.55±0.09) kg(P=0.001)화기저절경사조적(2.50±0.12)kg(P =0.017).피질+기저절경사조전선치입심도위(5.59 ±0.24)cm,현저소우주망막하강출혈조적(6.00 ±0.50)cm(P =0.036),단현저대우무병조조적(5.20 ±0.50) cm(P =0.033).모형제작후각조aEEG지간존재현저차이(F=14.059,P=0.000),피질+기저절경사조화기저절경사조조모후aEEG분별교모형제작전하강50.02％(=9.573,P＜0.001)화14.20％(=2.908,P=0.027).결론 응용선전법가성공건립표준화토M CAO모형,aEEG현저강저제시MCAO모형제작성공,병조루급피질.
Objective To establish a standardized middle cerebral artery occlusion (MCAO) model with suture method in rabbits and to investigate the value of the assessment for cerebral ischemia with amplitudeintegrated electroencephalogram (aEEG).Methods A total of 34 male New Zealand white rabbits were randomly assigned to either an MCAO group (n =29) or a sham operation goup (n =5).A model of MCAO was induced with intraluminal suture method and the cerebral function monitor was performed.According to 2,3,5-triphenyltetrazolium chloride staining,the MCAO group was further divided into cortex + basal ganglia infarction,basal ganglia infarction,subarachnoid hemorrhage,and non-lesion subgroups.The differences among the physiological indicators,weight,thread end diameter,and insertion length were compared before and after modeling in all subgroups.Results The success rate of MCAO modeling with suture method in rabbits was 62.07％ (18/29),in which 37.93％ (11/29) involved in the cortex and basal ganglia,24.38％ (7/29) only involved in the basal ganglia,17.24％ (5/29) complicated by subarachnoid hemorrhage,and 20.69％ (6/29) had no infarction.There were no significant differences in the body temperature,heart rate,mean arterial pressure and arterial blood pH,oxygen partial pressure,and CO2 partial pressure among all the subgroups before and after modeling.The weight in the non-lesion subgroup was 2.36 ± 0.10 kg,it was significantly lower than 2.55 ± 0.09 kg in the cortex + basal ganglia infarction subgroup (P =0.001) and 2.50 ± 0.12 kg in the basal ganglia infarction subgroup (P =0.017).The length of suture placement in the cortex+basal ganglia infarction subgroup was 5.59 ± 0.24 cm,and it was significantly less than 6.00 ± 0.50 cm in the subarachnoid hemorrhage subgroup (P =0.036).However,it was significantly longer than 5.20 ± 0.50 cm in the non-lesion subgroup (P =0.033).After modeling there were significant differences in aEEG among all subgroups (F =14.059,P =0.000).Compared to before modeling,aEEG decreased 50.02％ (t =9.573,P ＜ 0.001) and 14.20％ respectively after modeling in the cortex + basal ganglia infarction subgroup and the ganglia infarction subgroup (t =2.908,P =0.027).Conclusions A standardized MCAO model in rabbits may be successfully established with suture method.The significantly decreased aEEG indicates that the MCAO model is successful and the lesions involve in the cortex.