国际遗传学杂志
國際遺傳學雜誌
국제유전학잡지
INTERNATIONAL JOURNAL OF GENETICS
2009年
6期
406-411
,共6页
寡核苷酸链%点突变%基因定位修复
寡覈苷痠鏈%點突變%基因定位脩複
과핵감산련%점돌변%기인정위수복
Oligonucleotide%Point mutation%Site-specific gene repair
应用特殊设计的寡核苷酸链纠正点突变技术是20世纪90年代发展起来的一项新技术,在酵母菌、植物细胞、哺乳动物细胞等不同的生物材料,甚至动物模型中都有越来越多成功的报道.该技术还处于探索阶段,其基因修复机制还不是很清楚,目前认为突变纠正率与寡核苷酸链的结构、转运体系、靶基因的转录、DNA的复制、错配修复、损伤修复等生物学过程有关.
應用特殊設計的寡覈苷痠鏈糾正點突變技術是20世紀90年代髮展起來的一項新技術,在酵母菌、植物細胞、哺乳動物細胞等不同的生物材料,甚至動物模型中都有越來越多成功的報道.該技術還處于探索階段,其基因脩複機製還不是很清楚,目前認為突變糾正率與寡覈苷痠鏈的結構、轉運體繫、靶基因的轉錄、DNA的複製、錯配脩複、損傷脩複等生物學過程有關.
응용특수설계적과핵감산련규정점돌변기술시20세기90년대발전기래적일항신기술,재효모균、식물세포、포유동물세포등불동적생물재료,심지동물모형중도유월래월다성공적보도.해기술환처우탐색계단,기기인수복궤제환불시흔청초,목전인위돌변규정솔여과핵감산련적결구、전운체계、파기인적전록、DNA적복제、착배수복、손상수복등생물학과정유관.
The technology that correcting the point mutation by specially designed oligonucleotides is a laest molecular biological technique developed from late 1990s'. Successful repair has been achieved in yeast,plant, mammalian cells and other biological material, even in more and more animal models. But the technology is still in its developing and growing stage, and the gene repair mechanisms need to be elucidated more exactly.At present, the following factors are considered to be related; the structures and transport systems of oligonucleotides, transcription of the target gene, DNA replication, mismatch repair, damage repair and other relate biological processes.
