CAJ | 학술논문

目的探讨DMBTl在人类肝硬化组织中的表达及其与卵圆细胞增殖的关系.方法对22例人肝硬化标本进行CK19、Hep和DMBT1免疫组化染色,用激光显微切割方法研究DMBT1在增生的胆小(PBD)中的缺失(LOH),同时对其中5例标本进行电镜观察·以10例正常肝脏作对照.结果光镜下,所有肝硬化组织中均可找到少量卵圆细胞,其数量与炎症程度成正相关,电镜下证实卵圆细胞位于PBD中.DMBT1在卵圆中细胞也有表达,其在PBD中的阳性率为45.5%.LOH分析发现DMBT1在PBD中的发生率为45.5%,与周围肝细胞相比有显著性差异(P<0.05).而正常肝组织中未找到双向表达的卵圆细胞,仅少量大胆管有DMBTl的表达.结论人肝硬化组织中存在双向分化的卵圆细胞,位于PBD中,DMBT1在此类细胞中高表达,DMBT1 LOH较特异的出现在PBD中,并与蛋白表达呈现相对一致性,说明DMBT1可能参与卵圆细胞的分化增殖,在肝硬化的发生与发展中可能起到调控作用.
목적 탐토DMBTl재인류간경화조직중적표체급기여란원세포증식적관계.방법 대22례인간경화표본진행CK19、Hep화DMBT1면역조화염색,용격광현미절할방법연구DMBT1재증생적담소(PBD)중적결실(LOH),동시대기중5례표본진행전경관찰·이10례정상간장작대조.결과 광경하,소유간경화조직중균가조도소량란원세포,기수량여염증정도성정상관,전경하증실란원세포위우PBD중.DMBT1재란원중세포야유표체,기재PBD중적양성솔위45.5%.LOH분석발현DMBT1재PBD중적발생솔위45.5%,여주위간세포상비유현저성차이(P<0.05).이정상간조직중미조도쌍향표체적란원세포,부소량대담관유DMBTl적표체.결론 인간경화조직중존재쌍향분화적란원세포,위우PBD중,DMBT1재차류세포중고표체,DMBT1 LOH교특이적출현재PBD중,병여단백표체정현상대일치성,설명DMBT1가능삼여란원세포적분화증식,재간경화적발생여발전중가능기도조공작용.
Objective To investigate the expression of DMBT1 in liver cirrhosis and its relation to oval cell proliferation.Methods Immunostaining was carried out for CK19, Hep and DMBT1 in 22 liver cirrhotic samples.The homozygous deletion of DMBT1 in proliferated bile ductules (PBD) was investigated by laser capture microdissection (LCM).Meanwhile, the samples from 5 cases were ex-amined with transmission electron microscopy.Ten normal liver tissues were used as controls.Results Microscopically, oval cells could be found in all cirrhotic cases which could coexpress CK19 and Hep and its number was found to increase significantly with the progression of inflammation.Electron mi-croscope identified the presence of oval cells in PBD.DMBT1 could express in these cells too and its positive rate in PBD was 45.5 %.The homozygous deletion of DMBT1 was found in 45.5% PBD.In normal liver tissues, PBD and oval cells were not found.DMBT1 expression was only detectable in few large bile ducts.Conclusion There are bipotent oval cells with high expression of DMBT1 in liver cirrhosis hiding in PBD and DMBT1 can be specifically deleted in PBD, suggesting that DMBT1 may be related to the proliferation of oval cells and may participate in the development of liver cirrhosis.