CAJ | 학술논문

目的探讨紫外线蓝光混合光照射血浆(碱化或未碱化)降低胆红素浓度效率,建立净化人工肝分离血浆新方法.方法 ①将人工肝分离出的血浆用Na_2CO_3碱化至pH10,再用紫外线蓝光混合光照射15 min(碱化血浆照射组),检测胆红索和蛋白浓度.②将人工肝分离出的血浆直接用紫外线监光混合光照射15 min(未碱化血浆照射组),检测胆红素和蛋白浓度.结果在碱化血浆照射组,总胆红素(TBil)、直接胆红素(DBil)、间接胆红素(IBil)清除率分别为85%、84%、86%;在未碱化血浆照射组,TBil、DBil、IBil清除率分别为50%、50%、49%.两组TBil、DBil、IBil清除率差异都有统计学意义(P<0.01).两组的白蛋白回收率分别为99%和97%.结论用紫外线蓝光混合光照射碱化或未碱化血浆都可明显降低胆红素浓度,尤以碱化血浆照射组胆红素清除率更高,且白蛋白回收收率达97%～99%,有单用于人工肝血液净化.
목적 탐토자외선람광혼합광조사혈장(감화혹미감화)강저담홍소농도효솔,건립정화인공간분리혈장신방법.방법 ①장인공간분리출적혈장용Na_2CO_3감화지pH10,재용자외선람광혼합광조사15 min(감화혈장조사조),검측담홍색화단백농도.②장인공간분리출적혈장직접용자외선감광혼합광조사15 min(미감화혈장조사조),검측담홍소화단백농도.결과 재감화혈장조사조,총담홍소(TBil)、직접담홍소(DBil)、간접담홍소(IBil)청제솔분별위85%、84%、86%;재미감화혈장조사조,TBil、DBil、IBil청제솔분별위50%、50%、49%.량조TBil、DBil、IBil청제솔차이도유통계학의의(P<0.01).량조적백단백회수솔분별위99%화97%.결론 용자외선람광혼합광조사감화혹미감화혈장도가명현강저담홍소농도,우이감화혈장조사조담홍소청제솔경고,차백단백회수수솔체97%～99%,유단용우인공간혈액정화.
Objective To study the clearance rates of bilirubins by ultraviolet-blue ray irradiation on the alkalinized plasma or non-alkalinized plasma,to develop a new method to purify the plasma from artificial liver treatment.Methods The plasma from artificial liver treatment were alkalinized to pH10,and irradiated by the ultraviolet-blue ray to decrease levels of bilirubins (group A).The plasma from artificial liver treatment were irradiated by the ultraviolet-blue ray to decrease levels of bilirubins (group B).Results For group A,the clearance rates of total,direct and indirect bilirubins(TBil,DBil,IBil)were 85% ,84% ,86% respectively,recovery of ALB was 99% .For group B,the clearance rates of TBil,DBil,IBil were 50% ,50% ,49% respectively,recovery of ALB was 97% .There was statistical difference in the clearance rates of TBil,DBil,IBil between the two groups (P < 0.01).Conclusions The levels of bilirubins could be decreased significantly by the ultraviolet-blue ray irradiaton on the alkalinized or non-alkalinized plasma,and the recovery of ALB is 97% - 99% .The clearance rates of TBil,DBil,IBil of the alkalinized plasma group are higher than that of non-alkalinized group.This new method may be useful to purify the plasma from artificial liver treatment.