CAJ | 학술논문

目的评价加兰他敏对大鼠心肌缺血再灌注损伤的影响.方法成年雄性SD大鼠50只,体重225 ～ 275 g,采用随机数字表法,将其随机分为5组,每组10只.假手术组(SH组)仅穿线不结扎；缺血再灌注组(IR组)采用结扎左冠状动脉前降支30 min后再灌注120 min的方法制备大鼠心肌缺血再灌注模型.SH组和IR组于缺血前30 min股静脉缓慢注射生理盐水2 ml/kg;加兰他敏组(GAL组)于缺血前30 min股静脉缓慢注射加兰他敏4 mg/kg,余处理同IR组；加兰他敏复合M受体拮抗剂阿托品组(AT组)缺血前45 min给予阿托品4 mg/kg,余处理同GAL组.加兰他敏联合迷走神经切断组(VGT组)缺血前45 min切断双侧颈迷走神经,余处理同GAL组.于再灌注120 min时处死大鼠取心脏,采用TTC法测定心肌梗死范围,计算心肌梗死区质量百分比,检测心肌组织髓过氧化物酶(MPO)和超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量.结果与SH组比较,其余组心肌梗死区质量百分比、MPO活性及MD含量升高,SOD活性降低(P＜0.05)；与IR组比较,GAL组心肌梗死区重量百分比、MPO活性及MDA含量降低,心肌SOD活性升高(P＜0.05)；与GAL组比较,AT组和VGT组心肌梗死区重量百分比升高,心肌MPO活性及MDA含量升高,SOD活性降低(P＜0.05).结论加兰他敏预处理可减轻心肌缺血再灌注损伤,其机制可能与调节外周迷走神经活性有关.
목적 평개가란타민대대서심기결혈재관주손상적영향.방법 성년웅성SD대서50지,체중225 ～ 275 g,채용수궤수자표법,장기수궤분위5조,매조10지.가수술조(SH조)부천선불결찰；결혈재관주조(IR조)채용결찰좌관상동맥전강지30 min후재관주120 min적방법제비대서심기결혈재관주모형.SH조화IR조우결혈전30 min고정맥완만주사생리염수2 ml/kg;가란타민조(GAL조)우결혈전30 min고정맥완만주사가란타민4 mg/kg,여처리동IR조；가란타민복합M수체길항제아탁품조(AT조)결혈전45 min급여아탁품4 mg/kg,여처리동GAL조.가란타민연합미주신경절단조(VGT조)결혈전45 min절단쌍측경미주신경,여처리동GAL조.우재관주120 min시처사대서취심장,채용TTC법측정심기경사범위,계산심기경사구질량백분비,검측심기조직수과양화물매(MPO)화초양화물기화매(SOD)활성급병이철(MDA)함량.결과 여SH조비교,기여조심기경사구질량백분비、MPO활성급MD함량승고,SOD활성강저(P＜0.05)；여IR조비교,GAL조심기경사구중량백분비、MPO활성급MDA함량강저,심기SOD활성승고(P＜0.05)；여GAL조비교,AT조화VGT조심기경사구중량백분비승고,심기MPO활성급MDA함량승고,SOD활성강저(P＜0.05).결론 가란타민예처리가감경심기결혈재관주손상,기궤제가능여조절외주미주신경활성유관.
Objective To investigate the effects of galantamine on the myocardial ischemia-reperfusion (I/R) injury in rats and the possible mechanism.Methods Fifty male SD rats weighing 225-275 g were randomly assigned into 5 groups (n =10 each):sham operation group (group SH); I/R group; galantamine + I/R group (group GAL); M receptor antagonist atropine + galantamine + I/R group (group AT); vagus nerve cut-off + galantamine + I/R group (group VGT).Myocardial I/R was induced by occlusion of left anterior descending branch of coronary artery for 30 min followed by 120 min reperfusion.Normal saline 2 ml/kg was slowly injected via the femoral vein at 30 min before ischemia in groups SH and I/R.Galantamine 4 mg/kg was slowly injected via the femoral vein at 30 min before ischemia in group GAL.Atropine 4 mg/kg was slowly injected via the femoral vein at 45 min before ischemia in group AT and the other procedures were the same as those in group GAL.Bilateral cervical vagus nerves were cut off at 45 min before ischemia in group VGT and the other procedures were the same as those in group GAL.At the end of reperfusion,the hearts were removed for determination of myocardial infarct size,MPO and SOD activities,and MDA contents.Results The myocardial infarct size was significantly larger,the MPO activity and MDA content were significantly higher,and the SOD activity was significantly lower in group I/R than in group SH,and in groups AT and VGT than in group GAL (P ＜ 0.05).The myocardial infarct size was significantly smaller,the MDA content and MPO activity were significantly lower,and the SOD activity was significantly higher in group GAL than in group I/R P ＜ 0.05).Conclusion Galantamine has protective effect on myocardium against I/R injury and regulation of peripheral vagus nerve tension may be involved in the mechanism.