CAJ | 학술논문

目的探讨应用MB49细胞建立3种膀胱癌模型及其最佳实验性治疗时机的选择.方法采用MB49细胞2×106/50μl皮下注射建立膀胱癌皮下模型;5×105/50μl进行膀胱灌注可以建立膀胱癌原位模型;1×105/200μl通过尾静脉注射可以建立膀胱痛肺转移模型.同时通过对模型的观察可以确立模型建立的时间及治疗时机的最佳选择.结果皮下肿瘤模型在种植后第5天可以触及到实质性肿块,这是实验性治疗的最佳时间.通过向膀胱内灌注肿瘤细胞可以获得比较稳定的原位模型,而术后1～3 d是实验性治疗的最佳时机.观察期间限定在3周以内.膀胱癌肺转移模型可以通过向鼠尾静脉内注射肿瘤细胞而得到,实验性治疗的时机可以选择在术后10 d以内,观察期间也在3周以内.结论用MIM9细胞可以建立膀胱癌的皮下模型,原位模型以及肺转移模型.模型的质量比较稳定,肿瘤的产生时间比较固定,可以获得实验性治疗时机的最佳选择.
목적 탐토응용MB49세포건립3충방광암모형급기최가실험성치료시궤적선택.방법 채용MB49세포2×106/50μl피하주사건립방광암피하모형;5×105/50μl진행방광관주가이건립방광암원위모형;1×105/200μl통과미정맥주사가이건립방광통폐전이모형.동시통과대모형적관찰가이학립모형건립적시간급치료시궤적최가선택.결과 피하종류모형재충식후제5천가이촉급도실질성종괴,저시실험성치료적최가시간.통과향방광내관주종류세포가이획득비교은정적원위모형,이술후1～3 d시실험성치료적최가시궤.관찰기간한정재3주이내.방광암폐전이모형가이통과향서미정맥내주사종류세포이득도,실험성치료적시궤가이선택재술후10 d이내,관찰기간야재3주이내.결론 용MIM9세포가이건립방광암적피하모형,원위모형이급폐전이모형.모형적질량비교은정,종류적산생시간비교고정,가이획득실험성치료시궤적최가선택.
Objective To establish three types of bladder cancer models with MB49 cells and investigate the optimal opportunity for experimental treatment. Methods The subcutaneous model of bladder cancer was established with 2×106 cells/50 uX MB49 cells;orthotopic model with 5×105 cells/50 μlMB49 cells through catheter, and pulmonary metastatic model with 1×105 cells/200 μl MB49 cells via tail vein. The optimal opportunity for experimental treatment was chosen. Results It was at the 5th day after transplantation that palpable tumor could be detected in subcutaneous model, which was the optimal opportunity for experimental treatment. The steady orthotopic model could be established by cancer cell bladder perfusion. Fist to third day was the optimal opportunity for experimental treatment. The observation duration was 3 weeks. The pulmonary metastatic model could be established by trail intravenous injection. The optimal time for experimental treatment was within 7 days. The observation duration was also 3 weeks. Conclusion The subcutaneous model, orthotopic model and pulmonary metastatic model of bladder cancer could be established by MB49 cells. The quality of the models wap good, and the time of tumor development was steady. It was favorable for choosing the optimal time of e Derimental treatment.