解剖学报
解剖學報
해부학보
ACTA ANATOMICA SINICA
2010年
2期
252-256
,共5页
朱华萍%赵勇%赵洁%张远强
硃華萍%趙勇%趙潔%張遠彊
주화평%조용%조길%장원강
促甲状腺激素释放激素受体%二甲磺基乙烷%睾丸%Leydig细胞%免疫印迹法%免疫组织化学%大鼠
促甲狀腺激素釋放激素受體%二甲磺基乙烷%睪汍%Leydig細胞%免疫印跡法%免疫組織化學%大鼠
촉갑상선격소석방격소수체%이갑광기을완%고환%Leydig세포%면역인적법%면역조직화학%대서
Thyrotropin-releasing hormone receptor%Ethane dimethanesulphonate%Testis%Leydig cell%Western blotting%Immunohistochemistry%Rat
目的 探讨促甲状腺激素释放激素受体-1,-2(TRH-R1,TRH-R2)在二甲磺酸乙烷(EDS)处理大鼠睾丸中的表达及其在Leydig细胞中表达的意义.方法 构建30只EDS处理大鼠模型,应用蛋白质免疫印迹杂交技术、免疫组织化学ABC法以及免疫荧光双标法,检测TRH-R1和TRH-R2在EDS处理后2d、7d、14 d、21d和28d大鼠睾丸中的表达和定位. 结果 免疫印迹杂交发现,EDS处理后2d至14d,大鼠睾丸组织未见TRH-R1和TRH-R2表达,在EDS处理后21d、28d再次检测到了TRH-R1和TRH-R2的表达;免疫组织化学染色显示,TRH-R1和TRH-R2表达于EDS处理后21d、28d大鼠睾丸生精小管周围的长梭形细胞,免疫荧光双标证实此类细胞为再生的Leydig祖细胞. 结论 TRH-R1和TRH-R2参与了Leydig细胞的再生过程,可能对成年型Leydig细胞的发育、分化具有调节作用.
目的 探討促甲狀腺激素釋放激素受體-1,-2(TRH-R1,TRH-R2)在二甲磺痠乙烷(EDS)處理大鼠睪汍中的錶達及其在Leydig細胞中錶達的意義.方法 構建30隻EDS處理大鼠模型,應用蛋白質免疫印跡雜交技術、免疫組織化學ABC法以及免疫熒光雙標法,檢測TRH-R1和TRH-R2在EDS處理後2d、7d、14 d、21d和28d大鼠睪汍中的錶達和定位. 結果 免疫印跡雜交髮現,EDS處理後2d至14d,大鼠睪汍組織未見TRH-R1和TRH-R2錶達,在EDS處理後21d、28d再次檢測到瞭TRH-R1和TRH-R2的錶達;免疫組織化學染色顯示,TRH-R1和TRH-R2錶達于EDS處理後21d、28d大鼠睪汍生精小管週圍的長梭形細胞,免疫熒光雙標證實此類細胞為再生的Leydig祖細胞. 結論 TRH-R1和TRH-R2參與瞭Leydig細胞的再生過程,可能對成年型Leydig細胞的髮育、分化具有調節作用.
목적 탐토촉갑상선격소석방격소수체-1,-2(TRH-R1,TRH-R2)재이갑광산을완(EDS)처리대서고환중적표체급기재Leydig세포중표체적의의.방법 구건30지EDS처리대서모형,응용단백질면역인적잡교기술、면역조직화학ABC법이급면역형광쌍표법,검측TRH-R1화TRH-R2재EDS처리후2d、7d、14 d、21d화28d대서고환중적표체화정위. 결과 면역인적잡교발현,EDS처리후2d지14d,대서고환조직미견TRH-R1화TRH-R2표체,재EDS처리후21d、28d재차검측도료TRH-R1화TRH-R2적표체;면역조직화학염색현시,TRH-R1화TRH-R2표체우EDS처리후21d、28d대서고환생정소관주위적장사형세포,면역형광쌍표증실차류세포위재생적Leydig조세포. 결론 TRH-R1화TRH-R2삼여료Leydig세포적재생과정,가능대성년형Leydig세포적발육、분화구유조절작용.
Objective To investigate the expression of thyrotropin-releasing hormone receptor (TRH-R) type-1and type-2 in ethane dimethanesulphonate (EDS)-treated rat testis, and to discuss the significance of its expression in Leydig cells.Methods To make the injured testis Leydig cells rat model with EDS treatment. Western blotting, immunohistochemical ABC and immunofluorescence double labeling methods were used to detect the expression and location of TRH-R1 and R2 in the testicular tissues of EDS-treated-day 2,day 7,day 14,day 21 and day 28 rat mode, respectively. Results Western blotting results showed that the positive immunochemical staining was not found in the testicular tissues of the EDS-treated day 2 to day 14, on the other hand,they were found in EDS-treated-21 day and EDS-treated-28 day. Immunohistochemistry demonstrated that TRH-R1 and R2 expressed in the spindle-shaped cells reappeared around seminiferous tubules of post-EDS 21 days and 28 days groups. Immunofluorescence double labeling confirmed that these TRH-R1 and R2 positively stained cells were newly regenerated progenitor Leydig cells.Conclusion TRH-R1 and R2 are involved in the regeneration of Leydig cells in EDS-treated rat testis, and they may exert functions in the proliferation and differentiation of adult type Leydig cells.
