CAJ | 학술논문

目的:研究MMP-9在胃癌组织中的表达状况,分析其与临床病理特征和生存之间的关系.方法:应用免疫组化SP法,采用组织芯片技术,分析189例胃癌、54例癌旁组织、41例淋巴结转移灶及32例正常对照组织中c-Jun和MMP-9蛋白表达.结果:胃癌中MMP-9蛋白的阳性表达率为78.3%.MMP-9的表达与浸润深度、淋巴结转移、分化程度及Lauren分型有关(P<0.05).41例淋巴结转移灶和相对应的41例胃癌组织各因子表达比较发现,MMP-9阳性表达率具有显著性差异(P<0.05).与MVD进行比较发现,MMP-9阳性患者的MVD值高于阴性组,差异有统计学意义(P<0.05).单因素生存分析显示MMP-9阳性组对胃癌患者生存期的影响有统计学意义(P<0.05),Cox多因素回归分析显示MMP-9的异常表达不能作为影响胃癌预后的独立因素(P>0.05).结论:MMP-9在胃癌组织中均有高表达.MMP-9阳性表达与肿瘤的浸润、转移密切相关,可作为判断胃癌恶性行为的重要生物学标记物.MMP-9在促进血管生成中起重要作用.MMP-9蛋白阳性表达对胃癌患者来说是一个不利的预后因素,但尚不能作为一个独立的预后指标.
목적:연구MMP-9재위암조직중적표체상황,분석기여림상병리특정화생존지간적관계.방법:응용면역조화SP법,채용조직심편기술,분석189례위암、54례암방조직、41례림파결전이조급32례정상대조조직중c-Jun화MMP-9단백표체.결과:위암중MMP-9단백적양성표체솔위78.3%.MMP-9적표체여침윤심도、림파결전이、분화정도급Lauren분형유관(P<0.05).41례림파결전이조화상대응적41례위암조직각인자표체비교발현,MMP-9양성표체솔구유현저성차이(P<0.05).여MVD진행비교발현,MMP-9양성환자적MVD치고우음성조,차이유통계학의의(P<0.05).단인소생존분석현시MMP-9양성조대위암환자생존기적영향유통계학의의(P<0.05),Cox다인소회귀분석현시MMP-9적이상표체불능작위영향위암예후적독립인소(P>0.05).결론:MMP-9재위암조직중균유고표체.MMP-9양성표체여종류적침윤、전이밀절상관,가작위판단위암악성행위적중요생물학표기물.MMP-9재촉진혈관생성중기중요작용.MMP-9단백양성표체대위암환자래설시일개불리적예후인소,단상불능작위일개독립적예후지표.
0bjective:To investigate the expression of MMP-9 in gastric cancer,para-cancerous tissue and metastastic lymph nodes and to explore its relationship with the clinicopathologic features and prognosis.Methods:Immunohistochemis-try was used to detect the expression of MMP-9 on tissue microarray containing gastric normal mucosa(n=32),para-can-cerous tissue(n=54),metastastic lymph nodes(n=41),and gastric cancer tissue(n=189).Results:The positive rate of MMP-9 expression was 78.3% in gastric cancer tissues.The positive rate of MMP-9 expression was associated with depth of invasion,lymph node metastasis,Lauren type and degree of differentiation(P<0.05),but was not associated with sex,age,or tumor size(P>0.05).There was a significant difference in MMP-9 expression between gastric cancer tissues and metastastic lymph nodes(P<0.05).In metastastic lymph nodes,the positive rate of MMP-9 expression was higher.The MVD in MMP-9 positive tissues tended to be greater than that in MMP-9 negative tissues,with a statistical significance(P<0.05).Kaplan-Meier survival analysis showed that the survival of patients without MMP-9 expression was longer than those with positive MMP-9 expression(P<0.05).COX regression analysis revealed that MMP-9 expression was not an indepen-dent prognostic factor for gastric cancer.Conclusion:The higher expression of MMP-9 involves the occurrence,progres-sion,invasion,and metastasis of gastric cancer.MMP-9 can be used as a metastatic predictor and prognostic marker for gastric cancer.MMP-9 may promote angiogenesis.But MMP-9 is not an independent prognostic indicator for gastric cancer.