CAJ | 학술논문

目的探讨非霍奇金淋巴瘤(NHL)患者淋巴瘤细胞中环氧合酶-2(COX-2)和乳腺癌耐药蛋白(BCRP)表达的相关性及其意义.方法应用半定量反转录-聚合酶链式反应(RT-PCR)的方法,以β-actin作为内对照,检测61例NHL患者和10例反应增生性淋巴结患者(对照组)淋巴结组织的BCRP mRNA表达;应用免疫组织化学SP法对61例NHL患者和10例对照组淋巴结组织COX-2检测.结果 COX-2和BCRP在NHL中表达的阳性率分别为50.8%(31/61)和45.9%(28/61),明显高于对照组(P＜0.05).COX-2和BCRP在NHL中的表达呈正相关(x2=8.795,r=0.355,P＜0.05).COX-2和BCRP在NHL中的表达与临床、病理因素,如年龄、性别、国际预后指数(IPI)、乳酸脱氢酶(LDH)、β2-微球蛋白及Ann Arbor分期等无明显相关性(P＞0.05),但BCRP与化疗疗效明显相关.结论 BCRP可能在NHL多药耐药中发挥重要作用,有助于化疗疗效的评估及疾病预测;COX-2与NHL的多药耐药密切相关,COX-2抑制剂的使用,可能有助于提高患者对药物的敏感性.
목적 탐토비곽기금림파류(NHL)환자림파류세포중배양합매-2(COX-2)화유선암내약단백(BCRP)표체적상관성급기의의.방법 응용반정량반전록-취합매련식반응(RT-PCR)적방법,이β-actin작위내대조,검측61례NHL환자화10례반응증생성림파결환자(대조조)림파결조직적BCRP mRNA표체;응용면역조직화학SP법대61례NHL환자화10례대조조림파결조직COX-2검측.결과 COX-2화BCRP재NHL중표체적양성솔분별위50.8%(31/61)화45.9%(28/61),명현고우대조조(P＜0.05).COX-2화BCRP재NHL중적표체정정상관(x2=8.795,r=0.355,P＜0.05).COX-2화BCRP재NHL중적표체여림상、병리인소,여년령、성별、국제예후지수(IPI)、유산탈경매(LDH)、β2-미구단백급Ann Arbor분기등무명현상관성(P＞0.05),단BCRP여화료료효명현상관.결론 BCRP가능재NHL다약내약중발휘중요작용,유조우화료료효적평고급질병예측;COX-2여NHL적다약내약밀절상관,COX-2억제제적사용,가능유조우제고환자대약물적민감성.
Objective To investigate the correlation and significance of cyclooxygenase-2 (COX-2)and the breast cancer resistance protein (BCRP) expressions in non-Hodgkin lymphoma(NHL). Methods Ten patients with reactive lymph nodes (RLN) were considered as a control group. Compared with β-actin as internal control, the BCRP mRNA expressions of 61 NHL samples and 10 lymph tissues in control group were detected by semi-quantitative revere transcription polymerase chain reaction (RT-PCR) assay, while the expressions of COX-2 protein of the above specimens were detected by SP immunohistochemistry. Results The positive rates of COX-2 and BCRP in NHL were 50.8 %(31/61) and 45.8 % (28/61), respectively , and were higher than those in the control group (P ＜0.05). The expression of COX-2 was statistically positive correlated to that of BCRP (X2 =8.795, r=0.355, P＜0.05). The expressions of COX-2 and BCRP were not correlated to clinical and pathological factors, such as age, sex, IPI, LDH, β2-microglobulin level and Ann Arbor stage, however, the expression of BCRP was statistically correlated to chemotherapy efficacy.Conclusion BCRP may be involved in multi-drug resistance (MDR) of NHL, so it may contribute to the assessment of chemotherapy and prediction of NHL. Since there is a strong correlation between COX-2 expression and MDR in NHL, the application of COX-2 inhibitors may enhance sensitivity of chemotherapy.